A5023771717- Research on Leishmaniasis Studies
- Adenosine and Purinergic Signaling
- Trypanosoma species research and implications
- Biochemical and Molecular Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Parasites and Host Interactions
- Synthesis and Characterization of Heterocyclic Compounds
- Monoclonal and Polyclonal Antibodies Research
- Pineapple and bromelain studies
- Insect Pest Control Strategies
- Leptospirosis research and findings
- Pharmaceutical studies and practices
- Glycosylation and Glycoproteins Research
- Transgenic Plants and Applications
- Chemical synthesis and pharmacological studies
- Phagocytosis and Immune Regulation
- Enzyme Production and Characterization
- Animal health and immunology
- Infant Nutrition and Health
- Peptidase Inhibition and Analysis
- Science and Education Research
- Complement system in diseases
Universidade de São Paulo
2019-2023
Universidade de Ribeirão Preto
2023
Universidade Federal de Juiz de Fora
2009-2020
Coordenação de Aperfeicoamento de Pessoal de Nível Superior
2020
Fundação de Amparo à Pesquisa do Estado de Minas Gerais
2020
Nucleoside triphosphate diphosphohydrolase (NTPDase) activity was recently characterized in Leishmania (Viannia) braziliensis promastigotes (Lb), and an antigenic conserved domain (r82-121) from the specific NTPDase 1 isoform identified. In this work, mouse polyclonal antibodies produced against two synthetic peptides derived (LbB1LJ, r82-103; LbB2LJ, r102-121) were used. The anti-LbB1LJ or anti-LbB2LJ immobilized on protein A-sepharose immunoprecipitated of 48 kDa depleted approximately 40%...
A peptide (SmB2LJ; r175-194) that belongs to a conserved domain from Schistosoma mansoni SmATPDase 2 and is shared with potato apyrase, as predicted by in silico analysis antigenic, was synthesised its immunostimulatory property analysed. When inoculated BALB/c mice, this induced high levels of SmB2LJ-specific IgG1 IgG2a subtypes, detected enzyme linked immunosorbent assay. In addition, dot blots were found be positive for immune sera against apyrase SmB2LJ. These results suggest the...
Background Visceral Leishmaniasis in humans presents with fever, anemia, and splenomegaly can be lethal if not treated. Nevertheless, the majority of Leishmania infantum-infected individuals does manifest symptoms remain so provided they are immunosuppressed. In this work, performance different tests was evaluated to detect asymptomatic who were living Teresina, Piauí state, Brazil, an endemic area for VL. Methodology L. infantum-specific antibodies detected by ELISA two rapid...
SUMMARY An ATP diphosphohydrolase (EC 3.6.1.5) activity was identified in a Leishmania (Viannia) braziliensis promastigotes preparation (Lb). Ultrastructural cytochemical microscopy showed this protein on the parasite surface and also stained possible similar at mitochondrial membrane. Isolation of an active isoform from Lb obtained by cross-immunoreactivity with polyclonal anti-potato apyrase antibodies. These antibodies, immobilized Protein A-Sepharose, immunoprecipitated polypeptide...
Visceral leishmaniasis (VL) is a neglected disease considered serious public health problem, especially in endemic countries. Several studies have discovered monoxenous trypanosomatids (Leptomonas and Crithidia) patients with VL. In different situations of leishmaniasis, investigations examined cases co-infection between Leishmania spp. Crithidia These coinfections been observed wide range vertebrate hosts, indicating that they are not rare. Diagnostic techniques require improvements more...
The alkylaminoalkanethiosulfuric acids (AAATs) are amphipathic compounds effective against experimental schistosomiasis, of low toxicity, elevated bioavailability after a single oral dose and prompt tissue absorption.To explore the in-vitro antileishmanial potential AAATs using five this series Leishmania (Viannia) braziliensis.Their effects on promastigotes axenic amastigotes, cytotoxicity to macrophages were tested by MTT method, Leishmania-infected Giemsa stain. Effects mitochondrial...
Ecto-Nucleoside Triphosphate Diphosphohydrolases are enzymes that hydrolyze tri- and/or diphosphate nucleosides. Evidences pointed out to their participation in Trypanosoma cruzi virulence, infectivity and purine acquisition. In this study, recombinant T.cruzi knocking or overexpressing the TcNTPDase-1 gene were built, role of vitro interaction with VERO cells was investigated. Results show epimastigote forms hemi-knockout parasites showed about 50% lower level expression when compared wild...
Visceral leishmaniasis (VL) is a clinical form of with high mortality rates when not treated. Diagnosis suffers from invasive techniques and sub-optimal sensitivities. The current (affordable) treatment pentavalent antimony as advised by the WHO possibly harmful to patient. There need for an improved diagnosis prevent unnecessary treatment. N-glycan analysis may aid in diagnosis. We evaluated profiles active VL, asymptomatic infections (ASYMP) controls non-endemic (NC) endemic (EC) areas....
In kala-azar, immunoparalysis and the systemic inflammatory response are decisive parameters in pathogenesis of disease, predisposing individuals to infections bleeding, respectively. Monocytes (CD14+) expressing IL-10 extracellular IL-6 supporting factors for these outcomes. Additionally, certain cytokines can induce cell membrane exposure phosphatidylserine (PS), an aminophospholipid with ability modulate anti-inflammatory profile. A correlation was observed between percentage CD14+PS+...
Chagas Disease (CD) or American trypanosomiasis is a serious infectious disease that presents acute and chronic phases.In Brazil, cases of CD are compulsory notification to epidemiological surveillance.Between the years 2013 2017, in Piauí state, 350 were confirmed phase, which represent 26.8% registered Brazil (1304 cases).Therefore, screening phase paramount importance for controlling pathology.
As NTPDases (nucleosídeo trifosfato difosfohidrolases) são enzimas que hidrolisam nucleosídeos di- e trifosfatados sob ativação de íons bivalentes. Uma atividade NTPDásica foi caracterizada em promastigotas Leishmania infantum, um protozoário causador leishmaniose visceral.Neste trabalho, a NTPDase 1 purificada preparação usando como estratégia eletroforese gel não-desnaturante os detergentes deoxicolato sódio Triton X-100, quais preservam fosfohidrolítica desta enzima. Após eletroforese,...
A proteína nucleosídeo trifosfato difosfohidrolase 1 (NTPDase 1) de Leishmania(Viannia) braziliensis(Lb) foi recentemente identificada como antigênica e está presente em formas promastigota amastigota deste protozoário, sendo um possível alvo drogas. Compostos pertencentes à série dos ácidos N-alquilaminoalcanotiossulfúricos (AAATs), baixa toxicidade para mamíferos, foram caracterizados novos antileishmaniais. O estudo seus modos ação iniciado, neste trabalho os efeitos AAATs sobre a...