A5023653521- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Insect and Pesticide Research
- Toxin Mechanisms and Immunotoxins
- Effects of Environmental Stressors on Livestock
- Meat and Animal Product Quality
- Genetic and phenotypic traits in livestock
- vaccines and immunoinformatics approaches
- COVID-19 Clinical Research Studies
- Animal Disease Management and Epidemiology
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- Pharmacological Receptor Mechanisms and Effects
- Monoclonal and Polyclonal Antibodies Research
- Insect Resistance and Genetics
- Insect Pest Control Strategies
- T-cell and B-cell Immunology
- Genetic Mapping and Diversity in Plants and Animals
- Parasites and Host Interactions
- Animal Nutrition and Physiology
- Inflammasome and immune disorders
- Inflammation biomarkers and pathways
Universidade de São Paulo
2015-2024
Instituto Nacional de Metrologia, Qualidade e Tecnologia
2024
Centro Hospitalar Tondela-Viseu
2024
Universidade de Ribeirão Preto
2010-2023
Clinics Hospital of Ribeirão Preto
2008-2021
Fundação Faculdade de Medicina
2014
Brazilian Agricultural Research Corporation
1997-2010
Harvard University
2001
Dendritic cells (DCs) are powerful initiators of innate and adaptive immune responses. Ticks blood-sucking ectoparasite arthropods that suppress host immunity by secreting immunomodulatory molecules in their saliva. Here, compounds present Rhipicephalus sanguineus tick saliva with effects on DC differentiation, cytokine production, costimulatory molecule expression were identified. R. inhibited IL-12p40 TNF-α while potentiating IL-10 production bone marrow-derived DCs stimulated Toll-like...
Patients with COVID-19 predominantly have a respiratory tract infection and acute lung failure is the most severe complication. While molecular basis of SARS-CoV-2 immunopathology still unknown, it well established that associated hyper-inflammation tissue damage. Matrix metalloproteinases (MMPs) contribute to destruction in many pathological situations, activity MMPs leads release bioactive mediators inflammatory properties. We sought characterize scenario which could influence pathogenesis...
ABSTRACT Levels of the serum opsonin mannan-binding lectin (MBL) were directly correlated with probability developing visceral leishmaniasis. Monocytes infected MBL-opsonized Leishmania chagasi promastigotes secreted higher levels tumor necrosis factor alpha and interleukin-6 than cells nonopsonized parasites. Our findings indicate that MBL can modulate clinical outcome infection L. function macrophages.
The International Symposium on Leishmaniasis Vaccines, held in Olinda, Brazil, March 9–11, 2009, congregated international experts who conduct research vaccines against the leishmaniases. questions that were raised during meeting and ensuing discussions are compiled this report may assist guiding a agenda. A group to further discussion issues policy platform has been set up at http://groups.google.com/group/leishvaccines-l.
Abstract Background Rhipicephalus sanguineus , known as the brown dog tick, is a common ectoparasite of domestic dogs and can be found worldwide. R. recognized primary vector etiological agent canine monocytic ehrlichiosis babesiosis. Here we present first description salivary gland transcriptome by production analysis 2,034 expressed sequence tags (EST) from two cDNA libraries, one consctructed using mRNA dissected glands female ticks fed for 3-5 days (early to mid library, RsSGL1) another...
Tick salivary constituents antagonize inflammatory, immune and hemostatic host responses, favoring tick blood feeding the establishment of tick-borne pathogens in hosts during hematophagy. Amblyomma triste, A. cajennense parvum ticks are very important veterinary human health because they vectors etiological agents for several diseases. Insights into components involved essential to understanding vector-pathogen-host interactions, transcriptional profiling glands is a powerful tool do so....
Transcripts similar to those that encode the nonstructural (NS) proteins NS3 and NS5 from flaviviruses were found in a salivary gland (SG) complementary DNA (cDNA) library cattle tick Rhipicephalus microplus.Tick extracts cultured with cells enable isolation of viruses capable replicating invertebrate vertebrate cells. Deep sequencing viral RNA isolated culture supernatants provided complete coding sequences for their molecular characterisation confirmed similarity other flaviviruses....
The D7 subfamily of salivary proteins is widespread in blood sucking Diptera and belongs to the superfamily pheromone/odourant binding proteins. Although are among most abundant adult female mosquitoes sand flies, their role feeding remains elusive. In present work we report sequence seventeen novel proteins, propose an evolutionary scenario for appearance several forms this protein, based on a total twenty-one sequences from Culex quinquefasciatus, Aedes aegypti, Anopheles gambiae, An....
Ticks attach to and penetrate their hosts' skin inactivate multiple components of host responses in order acquire a blood meal. Infestation loads with the cattle tick, Rhipicephalus microplus, are heritable: some breeds carry high reproductively successful ticks, whereas others, few ticks feed reproduce efficiently. In elucidate mechanisms that result different outcomes infestations we examined global gene expression inflammation induced by tick bites skins from one resistant susceptible...
Visceral leishmaniasis (VL) can be lethal if untreated; however, the majority of human infections with etiological agents are asymptomatic. Using Illumina Bead Chip microarray technology, we investigated patterns gene expression in blood active VL patients, asymptomatic infected individuals, patients under remission and controls. Computational analyses based on differential expression, set enrichment, weighted co-expression networks cell deconvolution generated data demonstrating...
Ticks cause massive damage to livestock and vaccines are one sustainable substitute for the acaricides currently heavily used control infestations. To guide antigen discovery a vaccine that targets gamut of parasitic strategies mediated by tick saliva enables immunological memory, we exploited transcriptome constructed from salivary glands all stages Rhipicephalus microplus ticks feeding on genetically tick-resistant susceptible bovines. Different levels host anti-tick immunity affected gene...
Uncontrolled inflammatory responses play a critical role in coronavirus disease (COVID-19). In this context, because the triggering-receptor expressed on myeloid cells-1 (TREM-1) is considered an intrinsic amplifier of signals, study investigated soluble TREM-1 (sTREM-1) as biomarker severity and mortality COVID-19. Based their clinical scores, we enrolled COVID-19 positive patients (
Lipid and cholinergic mediators are inflammatory regulators, but their role in the immunopathology of COVID-19 is still unclear. Here, we used human blood tracheal aspirate (TA) to investigate whether acetylcholine (Ach), fatty acids (FAs), derived lipid (LMs) associated with severity. First, analyzed perturbation profile induced by SARS-CoV-2 infection transcriptional genes related ACh FA/LM pathways. Blood TA were for metabolomic lipidomic analyses quantification leukocytes, cytokines,...
Abstract Background Understanding the variation between well and poorly adapted cattle breeds to local environments pathogens is essential for breeding with improved climate disease-resistant phenotypes. Although considerable progress has been made towards identifying genetic differences breeds, at epigenetic chromatin levels remains characterized. Here, we generate, sequence analyse over 150 libraries base-pair resolution explore dynamics of DNA methylation accessibility bovine immune...
Visceral leishmaniasis (VL) is almost always lethal if not treated, but most infections with the causative agents are clinically silent. Mannan-binding lectin (MBL), an opsonin, a candidate molecule for modifying progression to VL because it may enhance infection intracellular pathogens. Mutations in MBL2 gene decrease levels of MBL and protect against development VL. This case-control study examines genotypes individuals presenting different outcomes Leishmania chagasi.Genotypes serum were...
Ticks secrete a cement cone composed of many salivary proteins, some which are rich in the amino acid glycine order to attach their hosts' skin. Glycine-rich proteins (GRPs) large family heterogeneous that have different functions and features; noteworthy adhesive tensile characteristics. These properties may be essential for successful attachment metastriate ticks host prolonged feeding necessary engorgement. In this work, we analyzed Expressed Sequence Tags (ESTs) similar GRPs from cDNA...
ABSTRACT Visceral leishmaniasis (VL) has a high fatality rate if not treated; nevertheless, the majority of human infections with causative agent, Leishmania infantum chagasi , are asymptomatic. Although VL patients often present increased levels serum immunoglobulins, contribution antibodies to resistance or progression disease remains unknown. Effector and regulatory functions rely on their interactions type I II Fc receptors, these tuned by patterns antibody N-glycosylation. In view...
MHC peptide binding and presentation is the most selective event defining landscape of T cell epitopes. Consequently, understanding diversity alleles in a given population parameters that define set ligands can be bound presented by each these (the immunopeptidome) has an enormous impact on our capacity to predict manipulate potential protein Ags elicit functional responses. Liquid chromatography-mass spectrometry analysis MHC-eluted ligand data proven powerful technique for identifying such...
Ticks (Acari: Ixodidae) are bloodsucking ectoparasitic arthropods of human and veterinary medical importance. Tick saliva has been shown to contain a wide range bioactive molecules with vasodilatory, antihemostatic, immunomodulatory activities. We have previously demonstrated that from Rhipicephalus sanguineus ticks inhibits the maturation dendritic cells (DCs) stimulated LPS. Here we examined mechanism this immune subversion, evaluating effect tick on Toll-like receptor (TLR)-4 signalling...
ABSTRACT Mogiana tick virus (MGTV) is a segmented jingmenvirus isolated in 2011 from cattle ticks Brazil. Here, we present complete coding genome sequence for MGTV isolate MGTV/V4/11, including all four segments. evolutionarily related to the Jingmen isolates SY84 and RC27.