A5046929557- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- vaccines and immunoinformatics approaches
- Immunotherapy and Immune Responses
- Actinomycetales infections and treatment
- Monoclonal and Polyclonal Antibodies Research
- Myxozoan Parasites in Aquatic Species
- Herpesvirus Infections and Treatments
- Infectious Diseases and Mycology
- Vector-borne infectious diseases
- RNA Interference and Gene Delivery
- Viral Infections and Immunology Research
- interferon and immune responses
- Animal Disease Management and Epidemiology
- Microbial infections and disease research
- Glycosylation and Glycoproteins Research
- Virus-based gene therapy research
- Animal Virus Infections Studies
- NF-κB Signaling Pathways
- Veterinary Equine Medical Research
- Viral Infections and Vectors
- Genetic and phenotypic traits in livestock
- Cytomegalovirus and herpesvirus research
- Zoonotic diseases and public health
- Rangeland Management and Livestock Ecology
Roslin Institute
2016-2024
University of Edinburgh
2016-2024
Genomics (United Kingdom)
2016-2017
Great progress has been made over recent years in the identification of selection signatures genomes livestock species. This work primarily carried out commercial breeds for which dominant pressures are associated with artificial selection. As agriculture and food security likely to be strongly affected by climate change, a better understanding environment-imposed on agricultural species is warranted. Ethiopia an ideal setting investigate environmental adaptation due its wide variation...
MHC peptide binding and presentation is the most selective event defining landscape of T cell epitopes. Consequently, understanding diversity alleles in a given population parameters that define set ligands can be bound presented by each these (the immunopeptidome) has an enormous impact on our capacity to predict manipulate potential protein Ags elicit functional responses. Liquid chromatography-mass spectrometry analysis MHC-eluted ligand data proven powerful technique for identifying such...
The major histocompatibility complex (MHC) region contains many genes that are key regulators of both innate and adaptive immunity including the polymorphic MHCI MHCII genes. Consequently, characterisation repertoire MHC is critical to understanding variation determines nature immune responses. Our current knowledge bovine limited with only Holstein-Friesian breed having been studied in any depth. Traditional methods genotyping low resolution laborious this has a impediment more...
Ovine pulmonary adenocarcinoma is a chronic respiratory disease of sheep caused by jaagsiekte retrovirus (JSRV). OPA significant economic problem for farmers in many countries and valuable animal model some forms human lung cancer. Here, we examined the changes host gene expression that occur response to JSRV infection. We identified large number genes with altered infected lung, including factors roles cancer immune system function. also compared data from previously published found degree...
The classical MHC class I and II molecules play key roles in determining the antigenic‐specificity of CD8+ CD4+ T‐cell responses—as such characterisation repertoire MHCI MHCII allelic diversity is fundamental to our ability understand, potentially, exploit how genetic influences outcome immune responses. Cattle remain one most economically livestock species, with particular importance many small‐holder farmers low‐and‐middle income countries (LMICs). However, knowledge (BoLA) indigenous...
The major histocompatibility complex (MHC) contains many genes that play key roles in initiating and regulating immune responses. This includes the polymorphic MHCI MHCII present epitopes to CD8+ CD4+ T-cells, respectively. Consequently, characterisation of repertoire MHC is an important component improving our understanding genetic variation determines outcomes In cattle, (BoLA) research has predominantly focused on Holstein-Friesian animals (as most economically breed globally), although...
Domestic sheep (Ovis aries) have been an important component of livestock agricultural production for thousands years. Preserving genetic diversity within populations maintains a capacity to respond changing environments and rapidly evolving pathogens. MHC can influence immune functionality at individual population levels. Here, we focus on defining functional class I haplotype in large cohort Scottish Blackface pre-selected high levels II DRB1 diversity. Using high-throughput amplicon...
The v-rel oncoprotein encoded by reticuloendotheliosis virus T strain (Rev-T) is a member of the rel/NF-κB family transcription factors capable transformation primary chicken spleen and bone marrow cells. Rapid avian haematopoietic cells occurs through process deregulation multiple protein-encoding genes its direct effect on their promoters. More recently, upregulation oncogenic miR-155 precursor pre-miR-155 was demonstrated in both Rev-T-infected embryo fibroblast cultures Rev-T-induced...
Marek's disease virus (MDV), an alphaherpesvirus of poultry, causes and is characterized by visceral CD4+TCRαβ+ T-cell lymphomas in susceptible hosts. Immortal cell lines harbouring the viral genome have been generated from ex vivo cultures MD tumours. As readily available sources large numbers cells, MDV-transformed lymphoblastoid (LCLs) are extremely valuable for studies virus-host interaction. While most cells held a latent state, minor populations display spontaneous reactivation...
One of the principal limitations on livestock productivity in sub-Saharan Africa is constraining effect infectious diseases, including tick-borne blood pathogens. Currently, diagnostic markers for these pathogens are species or genus specific, making it challenging to implement high-throughput screening methods. The aim this study was develop and validate a novel tool capable detecting range important haemopathogens livestock. To achieve this, we developed that can detect all
The Major Histocompatibility Complex (MHC) genes play a key role in number of biological processes, most notably immunological responses. MHCI and MHCII incorporate complex set highly polymorphic polygenic series genes, which, due to the technical limitations previously available technologies, have only been partially characterized non-model but economically important species such as horse. advent high-throughput sequencing platforms has provided new opportunities develop methods generate...
Abstract Major histocompatibility complex (MHC) peptide binding and presentation is the most selective event defining landscape of T cell epitopes. Consequently, understanding diversity MHC alleles in a given population parameters that define set ligands can be bound presented by each these (the immunopeptidome) has an enormous impact on our capacity to predict manipulate potential protein antigens elicit functional responses. Liquid chromatography-mass spectrometry (LC-MS) analysis eluted...
ABSTRACT Ovine pulmonary adenocarcinoma (OPA) is an infectious neoplastic lung disease of sheep caused by jaagsiekte retrovirus. OPA important veterinary problem and also a valuable large animal model for human adenocarcinoma. JSRV infects type 2 alveolar epithelial cells in the induces growth tumors, but little known about molecular events that lead to activation oncogenic pathways infected cells. MicroRNAs (miRNAs) are small RNA molecules approximately 22 nucleotides with roles regulating...