A5033361559- Zebrafish Biomedical Research Applications
- Developmental Biology and Gene Regulation
- Ichthyology and Marine Biology
- Congenital heart defects research
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- Physiological and biochemical adaptations
- Neurogenesis and neuroplasticity mechanisms
- Regulation of Appetite and Obesity
- MicroRNA in disease regulation
- Neuropeptides and Animal Physiology
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Marine animal studies overview
- Nerve injury and regeneration
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Reproductive biology and impacts on aquatic species
- Olfactory and Sensory Function Studies
- Hypothalamic control of reproductive hormones
- Aquaculture Nutrition and Growth
- Fish biology, ecology, and behavior
- Animal Behavior and Reproduction
- Connexins and lens biology
- Receptor Mechanisms and Signaling
- Aquaculture disease management and microbiota
Universidade de Santiago de Compostela
2015-2024
Centro de Investigaciones Biológicas Margarita Salas
2015
Although the distribution of cholinergic cells is remarkably similar across vertebrate species, no data are available on more primitive such as cartilaginous fishes. To extend evolutionary analysis systems, we studied neurons in brain and rostral spinal cord Scyliorhinus canicula by immunocytochemistry using an antibody against enzyme choline acetyltransferase (ChAT). Western blot extracts dogfish, sturgeon, trout, rat showed that this recognized bands four species. Putative were observed...
The distribution of cholinergic neurons and fibers was studied in the brain rostral spinal cord brown trout rainbow by using an antiserum against enzyme choline acetyltransferase (ChAT). Cholinergic were observed ventral telencephalon, preoptic region, habenula, thalamus, hypothalamus, magnocellular superficial pretectal nucleus, optic tectum, isthmus, cranial nerve motor nuclei, cord. In addition, new groups detected vascular organ lamina terminalis, parvocellular parts anterior tuberal a...
INTRODUCTIONDue to their large size and long generation times, chondrichthyans have been largely ignored by geneticists. However, key phylogenetic position makes them ideal subjects study the molecular bases of important morphological physiological innovations that characterize jawed vertebrates. Such analyses are crucial understanding origin complex genetic mechanisms unraveled in osteichthyans. The small spotted dogfish Scyliorhinus canicula, a representative largest order extant sharks,...
Left-right asymmetries in the epithalamic region of brain are widespread across vertebrates, but their magnitude and laterality varies among species. Whether these differences reflect independent origins forebrain or taxa-specific diversifications an ancient vertebrate feature remains unknown. Here we show that catshark Scyliorhinus canicula lampreys Petromyzon marinus Lampetra planeri exhibit conserved molecular between left right developing habenulae. Long-term pharmacological treatments...
Abstract Chondrychthyans (cartilaginous fishes) are key to understanding the ancestral gnathostome condition since they provide an outgroup sarcopterygians and actinopterygians. To gain comparative knowledge about development of vertebrate serotoninergic systems, we studied by immunohistochemistry origin, spatiotemporal organization, migration patterns serotonin‐containing neurons growth axonal pathways in central nervous system a shark, lesser spotted dogfish. Hindbrain...
Glycine and γ-aminobutyric acid (GABA) are the main inhibitory neurotransmitters in central nervous system (CNS) of vertebrates. Studies on distribution glycinergic neurons fibers have been carried out mainly rodents lampreys. With aim discovering more about early evolution this vertebrates, we analyzed glycine-immunoreactive (Gly-ir) CNS a basal ray-finned fish, Siberian sturgeon (Chondrostei, Acipenseriformes), by use immunohistochemical techniques. We also compared glycine GABA...
The saccus vasculosus (SV) is a circumventricular organ of the hypothalamus many jawed fishes whose functions have not yet been clarified. It vascularized neuroepithelium that consists coronet cells, cerebrospinal fluid-contacting (CSF-c) neurons and supporting cells. To assess organization, development evolution SV, expression glial fibrillary acidic protein (GFAP) neuronal markers γ-aminobutyric acid (GABA), glutamic decarboxylase (GAD; GABA synthesizing enzyme), neuropeptide Y (NPY),...
Pax6 is a highly conserved transcription factor that appears involved in the entire process of retinogenesis, including maintenance proliferation retinal progenitors and differentiation particular neuron fates. To gain insight into retinogenesis fish, we study dynamics expression developing mature retina two sharks inhabit environments, compare it with marker cell (proliferating nuclear antigen, PCNA) markers neuronal differentiation, such as glutamic acid decarboxylase (GAD), calretinin...
The olfactory system represents an excellent model for studying different aspects of the development nervous ranging from neurogenesis to mechanisms axon growth and guidance. Important findings in this field come comparative studies. We have analyzed key events shark Scyliorhinus canicula by combining immunohistochemical tract-tracing methods. describe first time a cartilaginous fish early population pioneer HuC/D-immunoreactive (ir) neurons that seemed delaminate pit epithelium migrate...
All studies to date of cholinergic systems bony fishes have been done in teleosts. To gain further insight into the evolution fishes, we studied brain a chondrostean fish, Siberian sturgeon (Acipenser baeri, Brandt), by using an antibody against choline acetyltransferase (ChAT). This study showed presence ChAT-immunoreactive (ChAT-ir) neurons preoptic region (parvocellular and magnocellular nuclei suprachiasmatic nucleus), periventricular tuberal hypothalamus, saccus vasculosus, dorsal...
We studied the development of primary olfactory system a teleost, brown trout, with aims clarifying whether caudal projection pertains to or terminal nerve system, identifying brain regions receiving this projection, and investigating its possible functional significance. As markers (OMs) we used two polyclonal antibodies (to substance P alpha-melanocyte-stimulating hormone) that were found label strongly after preadsortion antibody corresponding antigen (OMs), as marker an antiserum...
It is essential to consider chondrichthyans (cartilaginous fishes) in analyzing ancestral brain organization because this radiation represents the out-group all other living gnathostomes (jawed vertebrates). particularly crucial understand evolution of telencephalon chondrichthyans, as structure develops by evagination (as most vertebrates), whereas osteichthyans (bony fishes), it eversion, a markedly different process. Among Lesser Spotted Dogfish <i>Scyliorhinus canicula</i>...
The basic anatomy of the elasmobranch brain has been previously established after studying organization different subdivisions in adult brain. However, despite relatively abundant immunohistochemical and hodologic studies performed species sharks skates, some remains unclear. present study focuses on regions which basis anatomical data adults remain controversial, such as subpallium, mainly striatal subdivision. Taking advantage great potential lesser spotted dogfish, <i>Scyliorhinus...
Developmental studies of the central catecholaminergic (CA) system are essential for understanding its evolution. To obtain knowledge about CA in chondrichthyans, an ancient gnathostome group, we used immunohistochemical techniques detecting tyrosine hydroxylase (TH), initial rate-limiting enzyme synthesis, to study: 1) neuromery developing TH-immunoreactive (ir) neuronal populations, 2) development TH-ir innervation, and 3) organization cells fibers brain postembryonic stages shark...
The cerebellum is recognized as an evolutionary innovation of jawed vertebrates, whose most primitive group represented by the chondrichthyans, or cartilaginous fishes. A comprehensive knowledge cerebellar connections in these fishes might shed light on basal organization system. Although precerebellar system known adults, developmental studies are essential for understanding origin and evolution nuclei. In present work we performed a study embryos juveniles advanced shark species,...
Abstract To obtain a better understanding of the evolution brain catecholaminergic systems fishes, we have examined distribution catecholamine‐synthesizing enzymes in two species sturgeon ( Acipenser baeri and Huso huso ) using antibodies against tyrosine hydroxylase (TH) dopamine‐β ‐hydroxylase (DBH; only analyzed ). Both sturgeons showed TH‐immunoreactive (THir) neurons widely distributed most regions brain, highest number THir cells being located forebrain (olfactory bulb, preoptic area,...