This complete and fully assembled genome sequence of Mycoplasma bovis type strain PG45 is the first available for this species offers a framework comparison with additional pathogenic isolates. The single circular chromosome 1,003,404 bp reveals multiple gene sets mechanisms involved in variable expression surface antigens incursion numerous assorted mobile elements, despite its reduced size.

ABSTRACT Candidate partitioning genes ( parA and parB ) for the linear chromosome of Streptomyces coelicolor were identified by DNA sequencing in a series seven located between rnpA trxA near chromosomal replication origin. The most likely translation start point overlapped stop codon, suggestive coregulation, transcription analysis suggested that two formed an operon. Deletion part had no effect on growth or appearance colonies but caused deficiency during multiple septation events involved...

A 23-kb fragment of the Streptomyces coelicolor chromosome spanning dnaA region has been isolated as a cosmid clone. Nucleotide sequence analysis 5-kb portion shows that genes for RNase P protein (rnpA), ribosomal L34 (rpmH), replication initiator (dnaA), and beta subunit DNA polymerase III (dnaN) are present in highly conserved gene arrangement found all eubacterial genomes studied so far. The dnaA-dnaN intergenic is approximately 1 kb contains cluster at least 12 DnaA boxes with consensus...

ABSTRACT Staphylococcus hyicus and agnetis are two coagulase-variable staphylococcal species that can be isolated from bovine milk difficult to differentiate. The objectives of this study were characterize isolates origin a collection had previously been characterized as coagulase-positive S. based on phenotypic identification methods develop PCR-based method for differentiating , aureus . Isolates ( n = 62) selected previous in which samples collected cows 15 dairy herds. coagulase tested...

ABSTRACT The malp gene of Mycoplasma fermentans is shown to occur in single copy but encode two discrete translated forms lipid-modified surface protein that can be differentially expressed on isolates within this species: MALP-2, a 14-amino-acid (2-kDa) lipopeptide with potent macrophage-stimulatory activity (P. F. Mühlradt, M. Kiess, H. Meyer, R. Süssmuth, and G. Jung, J. Exp. Med. 185:1951–1958, 1997), MALP-404, an abundant, full-length (404-amino-acid) lipoprotein 41 kDa, previously...

Mycoplasma genomes contain compact gene sets that approach the minimal complement necessary for life and reflect multiple evolutionary instances of genomic reduction. Lateral transfer may play a critical role in shaping mobile pool these organisms, yet complex elements have not been reported within this genus. We describe here large ( approximately 23-kb) genetic element with unique features is present four copies fermentans PG18 chromosome, accounting 8% genome. These novel elements,...

Mycoplasma mycoides subsp. small colony biotype (SC) is the high-consequence animal pathogen causing contagious bovine pleuropneumonia. We report complete genome sequences of pathogenic strain M. SC Gladysdale and a close phylogenetic relative, leachii PG50(T), another clade.

Staphylococcus agnetis has been recently recognized as associated with disease in dairy cattle and meat-type chickens. The infections appear to be limited systemic broilers. This report details the molecular relationships between chicken isolates order understand how this species infects different hosts manifestations. data show that are very closely related, but all cluster together, suggesting a single jump from

ABSTRACT Variations in genome size and gene order were observed archival Salmonella enterica serovar Typhimurium cultures stored for over 40 years. In one strain, microarray analysis revealed a large, stable amplification. PCR of the same strain genomic duplication that underwent translocation. Other strains had smaller duplications deletions. These results demonstrate storage stabs time at room temperature not only allows further bacterial growth but also may produce an environment selects...

Self-resistance has been investigated in Streptomyces caelestis (producer of the lincosamide antibiotic celesticetin), from which a resistance determinant (clr) isolated on 1-kilobase DNA fragment and cloned lividans. The clr product is specific methylase produces single residue N6-monomethyladenine 23S rRNA at position 2058, thereby rendering 50S ribosmal subunit resistant to action lincosamides.

A new strategy for rapidly selecting and testing genetic vaccines has been developed, in which a whole genome library is cloned into bacteriophage lambda ZAP Express vector contains both prokaryotic (P(lac)) eukaryotic (P(CMV)) promoters upstream of the insertion site. The phage plated on Escherichia coli cells, immunoblotted, probed with hyperimmune and/or convalescent-phase antiserum to identify vaccine candidates. These are then plaque purified grown as liquid lysates, particles used...

ABSTRACT The generation of surface variation among many divergent species Mollicutes (mycoplasmas) occurs through stochastic expression patterns diverse lipoprotein genes. size and wide distribution such variable gene sets in minimal (∼0.6- to 1.4-Mb) mycoplasmal genomes suggest their key role the adaptation survival these wall-less monoderms. Diversity genes is less clearly established phylogenetically similar mycoplasmas, as Mycoplasma mycoides cluster ruminant pathogens, which vary widely...

Abstract CD8 + T cell tissue resident memory (T RM ) cells are especially suited to control pathogen spread at mucosal sites. However, their maintenance in lung is short-lived. TCR-dependent NFkB signaling crucial for but how and when modulates circulating during the immune response unknown. Here, we find that enhancing once influenza established, increases pro-survival Bcl-2 CD122 levels thus boosting maintenance. By contrast, signals contraction phase of leads a defect differentiation...

ABSTRACT Moraxella bovoculi is a recently identified species, recovered from the bovine eye, which under investigation as an etiological agent of infectious keratoconjunctivitis. A draft genome sequence type strain 237 T has been determined to identify features that may be important during host colonization.

Presented here is a draft genome sequence for Staphylococcus agnetis CBMRN 20813338, isolated from lactating dairy cow with subclinical mastitis. The approximately 2,416 kb and has 35.79% G+C content. Analysis of the deduced open reading frame (ORF) set identified candidate virulence attributes in addition to potential molecular targets species identification.

A new insertion sequence (IS) of Mycoplasma fermentans is described. This element, designated IS1630, 1,377 bp long and has 27-bp inverted repeats at the termini. single open reading frame (ORF), predicted to encode a basic protein either 366 or 387 amino acids (depending on start codon utilized), occupies most this compact element. The translation product ORF homology transposases IS30 family IS elements closely related (27% identical acid residues) prototype group, IS30. Multiple copies...

We previously isolated nucleic acid-binding antibody fragments (Fab) from bacteriophage display libraries representing the immunoglobulin repertoire of automimune mice to expedite analysis antibody-DNA recognition. In present study, binding properties one such anti-DNA Fab, high affinity single-stranded (ss) DNA-binding Fab (DNA-1), were defined using equilibrium gel filtration and fluorescence titration. Results demonstrated that DNA-1 had a marked preference for oligo(dT) (100 nM...

The approximately 16 kb genome of the Mycoplasma fermentans phiMFV1 prophage is described, and its mobility, replication effect on mycoplasma surface phenotype are demonstrated. In various M. strains, was either absent or integrated at diverse (and sometimes multiple) chromosomal sites, each marked by a conserved TTTTTA target sequence that duplicated upon integration. Precise excision, an extrachromosomal form loss from mycoplasmal were documented in series clonal derivatives propagated...