A5008377341- Ion channel regulation and function
- Cardiac Ischemia and Reperfusion
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Cardiac Arrest and Resuscitation
- Neuroscience of respiration and sleep
- Heart Rate Variability and Autonomic Control
- Nitric Oxide and Endothelin Effects
- Nicotinic Acetylcholine Receptors Study
- Fuel Cells and Related Materials
- Infectious Diseases and Tuberculosis
- Adenosine and Purinergic Signaling
- Cannabis and Cannabinoid Research
- Neuropeptides and Animal Physiology
- Hematological disorders and diagnostics
- Spinal Cord Injury Research
- Vestibular and auditory disorders
- Sepsis Diagnosis and Treatment
- Peripheral Artery Disease Management
- Tissue Engineering and Regenerative Medicine
- Lipid Membrane Structure and Behavior
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Electrolyte and hormonal disorders
- Acute Kidney Injury Research
- Congenital gastrointestinal and neural anomalies
Peking University
2017-2023
University of California, Davis
2008-2023
Peking University First Hospital
2017-2023
The First Affiliated Hospital, Sun Yat-sen University
2023
Sun Yat-sen University
2023
National Taiwan University Hospital
2012
Pain and Rehabilitation Medicine
2001-2007
University of California System
2007
St. Joseph's Hospital and Medical Center
2007
University of California, San Francisco
2000-2004
Little is known about the identity of endoplasmic reticulum (ER) export signals and how they are used to regulate number proteins on cell surface. Here, we describe two ER that profoundly altered steady-state distribution potassium channels were required for channel localization plasma membrane. When transferred other or a G protein-coupled receptor, these increased functional Thus, membrane not necessarily limited by folding assembly, but may be under control specific signals.
Background Cyclic GMP (cGMP)-dependent protein kinase (PKG) is recognized as an important signaling component in diverse cell types. PKG may influence the function of cardiac ATP-sensitive potassium (KATP) channels, ion channel critical for stress adaptation heart; however, underlying mechanism remains largely unknown. The present study was designed to address this issue. Methods and Findings Single-channel recordings KATP channels were performed both cell-attached inside-out patch...
Key points Both the ATP‐sensitive potassium (K ATP ) channel and gaseous messenger nitric oxide (NO) play fundamental roles in protecting heart from injuries related to ischaemia. NO has previously been suggested modulate cardiac K channels; however, underlying mechanism remains largely unknown. In this study, by performing electrophysiological biochemical assays, we demonstrate that potentiation of activity ventricular cardiomyocytes is prevented pharmacological inhibition soluble guanylyl...
The gamma-aminobutyric acidA (GABA)A receptor (GABAR) beta 1 and gamma 2L subtypes have been shown to be phosphorylated in vitro by protein kinase C (PKC) [J. Biol. Chem. 267:14470-14476 (1992); Neuron 12:1081-1095 (1994)]. To determine the physiological consequences of phosphorylation GABAR isoforms containing subtypes, specific serine residues PKC (beta S409, S327 S343) were changed alanines through site-directed mutagenesis. Wild-type (alpha GABARs) three mutant [alpha 2L(S327A, S343A),...
ATP-sensitive potassium (K ATP ) channels regulate insulin release, vascular tone, and neuronal excitability. Whether these are modulated by NO, a membrane-permeant messenger in various physiological pathological processes, is not known. The possibility of NO signaling via K channel modulation interest because both have been implicated functions such as vasodilation neuroprotection. In this report, we demonstrate mechanism that leads to activation NO/Ras/mitogen-activated protein kinase...
Potassium channels are widely distributed. To serve their physiological functions, such as neuronal signaling, control of insulin release, and regulation heart rate blood flow, it is essential that K + allow but not the smaller more abundant Na ions to go through. The narrowest part channel pore, selectivity filter formed by backbone carbonyls GYG-containing signature sequence, approximates hydration shell ions. However, sequence sufficient for selectivity. identify structural elements...
The ATP-sensitive potassium (K ATP ) channel couples intracellular metabolic state to membrane excitability. Recently, we demonstrated that neuronal K channels are functionally enhanced by activation of a nitric oxide (NO)/cGMP/cGMP-dependent protein kinase (PKG) signaling cascade. In this study, further investigated the mechanism underlying PKG stimulation channels. By performing single-channel recordings in transfected HEK293 and neuroblastoma SH-SY5Y cells, found increase Kir6.2/SUR1...
ATP-sensitive potassium (K ATP ) channels couple cellular metabolic status to changes in membrane electrical properties. Caffeine (1,2,7-trimethylxanthine) has been shown inhibit several ion channels; however, how caffeine regulates K was not well understood. By performing single-channel recordings the cell-attached configuration, we found that bath application of significantly enhanced currents Kir6.2/SUR1 channels, a neuronal/pancreatic channel isoform, expressed transfected human...
ATP-sensitive potassium (K ATP ) channels couple cell metabolic status to membrane excitability and are crucial for stress adaptation cytoprotection in the heart. Atrial natriuretic peptide (ANP), a cardiac important cardiovascular homeostasis, also exhibits cytoprotective features including protection against myocardial ischemia-reperfusion injuries. However, how ANP modulates K is largely unknown. In present study we sought address this issue by investigating role of signaling functional...
Sulfonylureas have been the leading oral antihyperglycemic agents, and they presently continue to be most popular antidiabetic drugs prescribed for treatment of type 2 diabetes. However, concern has arisen over side effects sulfonylureas on cardiovascular system. Here, we tested hypothesis that iptakalim, a novel vascular ATP-sensitive potassium (K<sub>ATP</sub>) channel opener, closes rat pancreatic β-cell K<sub>ATP</sub> channels increases insulin release. Rat heterologously expressed in...
SUMMARY 1. The inhibitory effects produced by activation of the medial region caudal medulla on activities left and right cardiac sympathetic, vagus greater splanchnic nerves were studied in chloralose‐urethane anaesthetized cats. 2. Electrical stimulation an 80–92% inhibition sympathetic nerve activities, a 45% 58% vagal respectively. There no significant differences between elicited autonomic nerves. Similar but smaller micro‐injection sodium glutamate (0.5 mol/L) or DL‐homocysteic acid...