A5066695908- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- SARS-CoV-2 and COVID-19 Research
- Polymer Surface Interaction Studies
- Dendrimers and Hyperbranched Polymers
- Immune responses and vaccinations
- Immunodeficiency and Autoimmune Disorders
- SARS-CoV-2 detection and testing
- COVID-19 Impact on Reproduction
Monash University
2020-2024
Peter Doherty Institute
2023
The University of Melbourne
2023
Abstract Cytosolic transport is an essential requirement but a major obstacle to efficient delivery of therapeutic peptides, proteins and nucleic acids. Current understanding cytosolic mechanisms remains limited due significant number conflicting reports, which are compounded by low sensitivity indirect assays. To resolve this, we develop highly sensitive Split Luciferase Endosomal Escape Quantification (SLEEQ) assay probe delivery. We apply SLEEQ evaluate the range widely studied...
All nanoparticles have the potential to revolutionize delivery of therapeutic cargo such as peptides, proteins, and RNA. However, effective cytosolic from represents a significant challenge in design more efficient drug vehicles. Recently, research has centered on designing with capacity escape endosomes by responding biological stimuli changes pH, which occur when are internalized into endo-/lysosomal pathway. Current endosomal assays rely indirect measurements yield little quantitative...
Polymer nanoparticles have generated significant interest as delivery systems for therapeutic cargo. Self-immolative polymers (SIPs) are an interesting category of materials applications, the characteristic property end-to-end depolymerization allows disintegration system, facilitating a more effective release cargo and clearance from body after use. In this work, based on pH-responsive polymer poly(ethylene glycol)-b-(2-diisopropyl)amino ethyl methacrylate) self-immolative...
Abstract Endosomal escape is an essential requirement but a major obstacle to efficient delivery of therapeutic peptides, proteins and nucleic acids. Current understanding endosomal mechanisms remains limited due significant number conflicting reports, which are compounded by low sensitivity indirect assays. To resolve this, we developed highly sensitive Split Luciferase Escape Quantification (SLEEQ) assay probe cytosolic delivery. We applied SLEEQ evaluate the range widely studied putative...
Abstract To address the limitations of whole-spike COVID vaccines, we explored mRNA vaccines encoding membrane-anchored receptor-binding domain (RBD-TMs), each a fusion variant RBD, transmembrane (TM) and cytoplasmic tail (CT) fragments SARS-CoV-2 spike protein. In naive mice, RBD-TM against ancestral SARS-CoV-2, Beta, Delta, Delta-plus, Kappa, Omicron BA.1 or BA.5, all induced strong humoral responses target RBD. Multiplex surrogate viral neutralization (sVNT) assays indicated broad...