A5100722683- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Infectious Diseases and Mycology
- Nail Diseases and Treatments
- Bacterial Identification and Susceptibility Testing
- Antibiotic Use and Resistance
- Protein purification and stability
- Enzyme Catalysis and Immobilization
- Mycobacterium research and diagnosis
- Yeasts and Rust Fungi Studies
- Fungal Biology and Applications
- COVID-19 impact on air quality
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Dental Research and COVID-19
- Pancreatic function and diabetes
- COVID-19 Pandemic Impacts
- Probiotics and Fermented Foods
- Medical and Biological Ozone Research
- HER2/EGFR in Cancer Research
- Actinomycetales infections and treatment
- COVID-19 epidemiological studies
- Oral Health Pathology and Treatment
- Parkinson's Disease Mechanisms and Treatments
Lady Hardinge Medical College
2023-2025
National Institutes of Health
2024
Texas Tech University
2024
First Technical University
2024
Rai University
2024
Rockefeller University
2023
Narsee Monjee Institute of Management Studies
2022
Indraprastha Institute of Information Technology Delhi
2020-2022
University of Delhi
2012-2020
Saifee Hospital
2018-2020
Candida auris is a multidrug-resistant yeast that causes wide spectrum of infections, especially in intensive care settings. We investigated C. prevalence among 102 clinical isolates previously identified as haemulonii or famata by the Vitek 2 system. Internal transcribed spacer region (ITS) sequencing confirmed 88.2% auris, and matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS) easily separated all related species, viz., (n = 90), 6), var. vulnera 1),...
Candida auris has emerged globally as an MDR nosocomial pathogen in ICU patients. We studied the antifungal susceptibility of C. isolates (n = 350) from 10 hospitals India collected over a period 8 years. To investigate azole resistance, ERG11 gene sequencing and expression profiling was conducted. In addition, echinocandin resistance linked to mutations FKS1 analysed. CLSI testing six azoles, amphotericin B, three echinocandins, terbinafine, 5-flucytosine nystatin Screening for amino acid...
A new clonal strain of Candida auris is an emerging etiologic agent fungemia in Delhi, India. In 12 patients 2 hospitals, it was resistant to fluconazole and genotypically distinct from isolates South Korea Japan, as revealed by M13 amplified fragment length polymorphism typing.
Summary In the last few years, infections caused by dermatophytes along with a concomitant increase in number of difficult to treat cases have increasingly been recognised, indicating that dermatophytosis remains challenging public health problem. The majority are Trichophyton rubrum and mentagrophytes complex. Terbinafine, an allylamine antifungal used orally topically is considered be first‐line drug therapy dermatophyte infections. Terbinafine resistance has predominately attributed point...
Azole resistance is an emerging problem in Aspergillus which impacts the management of aspergillosis. Here we report emergence and clonal spread to triazoles environmental fumigatus isolates India. A total 44 (7%) A. from 24 samples were found be triazole resistant. The isolation rate resistant was highest (33%) soil tea gardens followed by flower pots hospital garden (20%), beneath cotton trees rice paddy fields (12.3%), air wards (7.6%) admixed with bird droppings (3.8%). These strains...
Candidaemia is a potentially fatal infection with varied distribution of Candida species and their antifungal susceptibility profiles. The recent emergence auris in invasive candidiasis cause for concern. This study describes the profile candidaemia at an Indian tertiary care hospital reports C. auris. All patients diagnosed between 2012 2017 were studied. isolates identified using conventional methods, VITEK 2 MALDI-TOF MS. not by sequenced. Antifungal testing was done CLSI broth...
The emergence of fluconazole resistance in Candida parapsilosis healthcare-associated infections has recently been increasingly reported. Antifungal susceptibility profiles and mechanisms C. (n = 199) from nine hospitals India collected over a period 3 years were studied. Further, clonal transmission fluconazole-resistant isolates different was investigated.Antifungal testing five azoles, amphotericin B 5-flucytosine performed by the CLSI microbroth dilution method. azole target ERG11 gene...
Aspergillus species cause a wide spectrum of clinical infections. Although fumigatus and flavus remain the most commonly isolated in aspergillosis, last decade, rare cryptic have emerged diverse settings. The present study analyzed distribution vitro antifungal susceptibility profiles samples from patients with suspected aspergillosis 8 medical centers India. Further, matrix-assisted laser desorption ionization-time flight mass spectrometry in-house database was developed to identify these...
Aspergillus terreus is emerging as an etiologic agent of invasive aspergillosis in immunocompromised individuals several medical centers the world. Infections due to A. are concern its resistance amphotericin B, vivo and vitro, resulting poor response antifungal therapy high mortality. Herein we examined a large collection molecularly characterized, geographically diverse isolates (n = 140) from clinical environmental sources India for occurrence cryptic species. The population structure...
Nonsporulating molds (NSMs), especially basidiomycetes, have predominantly been reported as human pathogens responsible for allergic and invasive disease. Their conventional identification is problematic, many isolates remain sterile in culture. Thus, inconclusive culture reports might adversely affect treatment decisions. The clinical significance of NSMs pulmonary mycoses poorly understood. We sequenced the internal transcribed spacer (ITS) region D1/D2 domain larger subunit (LSU) 52...
Summary Mucormycosis is a highly aggressive disease which usually fatal in immunocompromised patients. The species of mucormycetes show significant differences susceptibility to amphotericin B, azoles and terbinafine. precise level identification for this fungal group could be achieved by internal transcribed‐spacer ( ITS ) region sequencing. Herein, we present the largest series antifungal data molecularly characterised isolates reported so far from I ndia. Eighty originating 71 patients...
Melanized or black fungi are a heterogeneous group of causing cutaneous to systemic diseases with high mortality. These rarely reported as agents human infections, primarily due difficulties in their classical identification. In this study, we examined, using molecular methods and matrix-assisted laser desorption ioniazation-time flight mass spectrometry (MALDI-TOF MS), the diversity melanized (MF) isolated from patients 19 medical centers India. Overall, during 4-year period, 718 (5.3%)...
ABSTRACT We compared EUCAST and CLSI antifungal susceptibility testing (AFST) methods for triazoles amphotericin B against 124 clinical Mucorales isolates. The method yielded MIC values 1- to 3-fold dilutions higher than those of the B. essential agreements between two were high, i.e., 99.1% (voriconazole), 98.3% (isavuconazole), 87% (posaconazole), whereas it was significantly lower (66.1%). Strategies harmonization AFST are warranted.
Coronavirus disease 2019 (COVID-19) pandemic has been prevailing for more than a year, associated with an increased number of opportunistic invasive fungal infections in patients who have critically ill or immunocompromised. In this retrospective study, details various clinical specimens received from suspected were studied. Fungal cultures positive 64% (51 out 79) COVID-19-positive and 43% (163 381) COVID-19-negative during the second wave COVID-19 2021. Among COVID-19-infected patients,...
ABSTRACT The filamentous basidiomycete Ceriporia lacerata , an agent of white rot on wood, has never been reported in human disease and its clinical significance is not yet known. We describe 4 patients with respiratory diseases where C. was implicated a wide spectrum manifestations ranging from saprobic colonization to fungal pneumonia. isolates did show the morphological characteristics that facilitate recognition basidiomycetes, such as presence clamp connections, spicules along hyphae,...
ABSTRACT Schizophyllum commune ( n = 30) showed lowest geometric mean MICs of isavuconazole (0.19 μg/ml), itraconazole (0.2 voriconazole (0.24 and amphotericin B (0.29 μg/ml) high fluconazole (19.39 flucytosine (17.28 μg/ml). Five cases (of 8) allergic bronchopulmonary mycosis that were treated with had no recrudescence after 6 to 24 months follow-up. One case each invasive pulmonary fungal ball successfully itraconazole.
Abstract Penicillium species are rarely reported agents of infections in immunocompromised patients. We report 3 cases invasive mycosis caused by voriconazole-resistant oxalicum patients with acute myeloid leukemia, diabetes mellitus, and chronic obstructive pulmonary disease, while on voriconazole therapy. has not been previously recognized as a cause mycoses.
A Burkholderia cepacia (bacteria) strain, A.T.C.C. 25609, which had been isolated from the bronchial washings of a cystic fibrosis patient, was used to produce lipase. The presence sodium alginate at an optimal concentration 8 mg.ml(-1) in growth medium nearly doubled production extracellular lipase activity. enzyme could be purified with 38-fold purification and 96% activity recovery using two-step protocol. molecular mass determined by SDS/PAGE shown 28 kDa. pH optimum 9 it stable up 12 h...
Abstract Many recombinant eukaryotic proteins tend to form insoluble aggregates called inclusion bodies, especially when expressed in Escherichia coli . We report the first application of technique three‐phase partitioning (TPP) obtain correctly refolded active from solubilized bodies. TPP was used for refolding 12 different overexpressed E. In each case, protein by gave either higher yield than earlier reported method or succeeded where efforts have failed. TPP‐refolded were characterized...