A5022622170- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Aging, Elder Care, and Social Issues
- Microbial Community Ecology and Physiology
- HIV, Drug Use, Sexual Risk
- Retirement, Disability, and Employment
- Additive Manufacturing and 3D Printing Technologies
- Opioid Use Disorder Treatment
- Genomics and Phylogenetic Studies
- Social Policy and Reform Studies
- Protist diversity and phylogeny
- Housing, Finance, and Neoliberalism
- Labor Law and Work Dynamics
- Hydrogels: synthesis, properties, applications
- Ichthyology and Marine Biology
- Social Policies and Family
- Species Distribution and Climate Change
- Cellular transport and secretion
- Microtubule and mitosis dynamics
- Coral and Marine Ecosystems Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Biosensing Techniques and Applications
- Employment and Welfare Studies
University College Dublin
2015-2024
Central European Institute of Technology
2024
Hôpital Lapeyronie
2021
Conway School of Landscape Design
2020
Scientific Centre of Monaco
2019
Universidad de Oviedo
2018
European Molecular Biology Laboratory
2005-2011
European Bioinformatics Institute
2002-2011
European Molecular Biology Laboratory
2009
Université Lumière Lyon 2
2008
Microbes are dominant drivers of biogeochemical processes, yet drawing a global picture functional diversity, microbial community structure, and their ecological determinants remains grand challenge. We analyzed 7.2 terabases metagenomic data from 243 Tara Oceans samples 68 locations in epipelagic mesopelagic waters across the globe to generate an ocean reference gene catalog with >40 million nonredundant, mostly novel sequences viruses, prokaryotes, picoeukaryotes. Using 139...
Marine plankton support global biological and geochemical processes. Surveys of their biodiversity have hitherto been geographically restricted not accounted for the full range size. We assessed eukaryotic diversity from 334 size-fractionated photic-zone communities collected across tropical temperate oceans during circumglobal Tara Oceans expedition. analyzed 18S ribosomal DNA sequences intermediate plankton-size spectrum smallest unicellular eukaryotes (protists, >0.8 micrometers) to small...
The structure, robustness, and dynamics of ocean plankton ecosystems remain poorly understood due to sampling, analysis, computational limitations. Tara Oceans consortium organizes expeditions help fill this gap at the global level.
Abstract The Tara Oceans expedition (2009–2013) sampled contrasting ecosystems of the world oceans, collecting environmental data and plankton, from viruses to metazoans, for later analysis using modern sequencing state-of-the-art imaging technologies. It surveyed 210 in 20 biogeographic provinces, over 35,000 samples seawater plankton. interpretation such an extensive collection their ecological context requires means explore, assess access raw validated sets. To address this challenge,...
While our knowledge about the roles of microbes and viruses in ocean has increased tremendously due to recent advances genomics metagenomics, research on marine microbial eukaryotes zooplankton benefited much less from these new technologies because their larger genomes, enormous diversity, largely unexplored physiologies. Here, we use a metatranscriptomics approach capture expressed genes open Tara Oceans stations across four organismal size fractions. The individual sequence reads cluster...
The mechanics of the actin cytoskeleton have a central role in regulation cells and tissues, but details how molecular sensors recognize deformations forces are elusive. By performing laser nanosurgery cultured epithelial fibroblasts, we show that retraction stress fibers (SFs) is restricted to proximity cut new adhesions form at retracting end. This suggests SFs attached substrate. A computational model for confirms this hypothesis predicts distribution propagation contractile along SF. We...
Light-sheet-based fluorescence microscopy (LSFM) is a technique that combines optical sectioning, the key capability of confocal and two-photon microscopes with multiple-view imaging, which used in tomography. In contrast to conventional wide-field microscopes, light sheet illuminates only focal plane detection objective lens from side. Excitation is, thus, restricted fluorophores volume near plane. This provides sectioning allows use regular cameras process. Compared microscopy, LSFM...
Although cells migrate in a constrained 3D environment vivo, in-vitro studies have mainly focused on the analysis of moving 2D substrates. Under such conditions, Golgi complex is always located towards leading edge cell, suggesting that it involved directional movement. However, several lines evidence indicate this location can vary depending cell type, or developmental processes. We used micro contact printing (microCP) to study migration geometrically shape within polarized phenotype....
Light microscopy enables researchers to observe cellular mechanisms with high spatial and temporal resolution. However, the increasing complexity of current imaging technologies, coupled financial constraints potential users, hampers general accessibility reach cutting-edge microscopy. Open can address this issue by making well-designed well-documented hardware software solutions openly available a broad audience. In Comment, we provide definition open present recent projects in field. We...
Coral reefs are the most diverse habitats in marine realm. Their productivity, structural complexity, and biodiversity critically depend on ecosystem services provided by corals that threatened because of climate change effects—in particular, ocean warming acidification. The coral holobiont is composed animal host, endosymbiotic dinoflagellates, associated viruses, bacteria, other microeukaryotes. In mandatory photosymbiosis with microalgae family Symbiodiniaceae its consequences evolution,...
Commercial live cell imaging systems represent a large financial burden to research groups, while current open source incubator microscopy lack adaptability and are sometimes inadequate for complex experimentation. We present here low-cost microscope designed inclusion within conventional tissue culture incubator. The build is constructed using an entry level 3D printer as the basis motion control system, with Raspberry Pi software integration, allowing reflected, oblique, fluorescence of...
Low-cost and scalable technologies that allow people to measure microplastics in their local environment could facilitate a greater understanding of the global problem marine microplastic pollution. A typical way pollution involves imaging filtered seawater samples stained with fluorescent dye aid detection microplastics. Although traditional fluorescence microscopy allows these particles be manually counted detected, this is resource- labour-intensive task. Here, we describe novel, low-cost...
Bacteria that also have features typical of eukaryotes and archaea may reflect a possible pathway in ancient cellular evolution.
Coral reefs hosts nearly 25% of all marine species and provide food sources for half a billion people worldwide while only very small percentage have been surveyed. Advances in technology processing along with affordable underwater cameras Internet availability gives us the possibility to tools softwares survey entire coral reefs. Holistic ecological analyses corals require not community view (10s 100s meters), but also single colony analysis as well corallite identification. As are...
Abstract With recent advances in the reprogramming of somatic cells into induced Pluripotent Stem Cells (iPSCs), gene editing technologies, and protocols for directed differentiation stem heterogeneous tissues, iPSC‐derived kidney organoids have emerged as a useful means to study processes renal development disease. Considerable guided by knowledge fundamental developmental signaling pathways been made with use exogenous morphogens generate more robust kidney‐like tissues vitro . However,...
Recent data have demonstrated the role of Cdk1- and Cdk2-dependent phosphorylation MyoDSer200 in regulation MyoD activity protein turnover. In present study, we show that presence p57Kip2, MyoDAla200, a mutant cannot be phosphorylated by cyclin-Cdk complexes, displayed 2–5-fold higher than MyoDAla200 alone transactivation muscle-specific genes myosin heavy chain, creatine kinase, light chain 1. Furthermore, p57Kip2 increases levels cotransfected cells. This result implies may regulate...
AbstractWe show that expression of p57Kip2, a potent tight-binding inhibitor several G1cyclin–cyclin-dependent kinase (Cdk) complexes, increases markedly during C2C12 myoblast differentiation. We examined the effect p57Kip2 on activity transcription factor MyoD. In transient transfection assays, transcriptional transactivation mouse muscle creatine promoter by MyoD was enhanced Cdk inhibitors. addition, p21Cip1, and p27Kip1 but not p16Ink4a induced an increased level protein, we MyoD,...
We designed a widefield frequency domain Fluorescence Lifetime Imaging Microscopy (FLIM)setup, which is based on Single Plane Illumination Microscope (SPIM). A SPIM provides an inherent optical sectioning capability and reduces photobleaching compared to conventional confocal fluorescence microscopes. The lifetime precision of the FLIM was characterized with Rhodamine 6G solutions different quencher concentrations [KI]. demonstrate high spatial resolution SPIM-FLIM combination in intensity...