Abstract Background This randomized clinical trial compared long-term outcome after antireflux surgery with acid inhibition therapy in the treatment of chronic gastro-oesophageal reflux disease (GORD). Methods Patients GORD and oesophagitis verified at endoscopy were allocated to omeprazole (154 patients) or (144). After 7 years follow-up, 119 patients arm 99 who had available for evaluation. The primary variable was cumulative proportion whom failed. Secondary objectives evaluation failure...

The ability of microbes to form biofilms is an important element their pathogenicity, and biofilm formation a serious challenge for today's medicine. Fighting the clinical complications associated with very difficult linked high risk failure, especially in time increasing bacterial resistance antibiotics. Bacterial species most commonly isolated from include coagulase-negative staphylococci, Staphylococcus aureus, Enterococcus faecalis, faecium, Escherichia coli, Proteus mirabilis,...

Background Among many sources of natural bioactive substances, mushrooms constitute a huge and almost unexplored group. Fungal compounds have been repeatedly reported to exert biological effects which prompted their use in pharmaceutical cosmetic industry. Therefore, the aim this study was analysis chemical composition activity 31 wild growing mushroom species (including saprophytic parasitic) from Poland. Methods Qualitative quantitative LC-ESI-MS/MS fourteen phenolic acids analysed...

Mushrooms have been part of human diet for centuries. They possess great potential both nutrition and therapeutic use. Amongst bioactive constituents occurring in mushrooms, phenolic compounds focus much attention due to their antioxidant activity. The aim the present study was determine chemical composition wild growing edible mushrooms traditionally eaten Poland. For this purpose, LC-ESI-MS/MS spectrophotometric tests were used. Our findings revealed presence benzoic acid (protocatechuic,...

According to the vast body of evidence demonstrating that intestinal microbiota is undoubtedly linked with overall health, including cancer risk, searching for functional foods and novel prebiotic influencing on beneficial bacteria necessary. The present study aimed investigate potential polysaccharides from 53 wild-growing mushrooms stimulate growth Lactobacillus acidophilus rhamnosus determine digestibility polysaccharide fractions.Mushroom were precipitated ethanol aqueous extracts....

Antimicrobial resistance (AMR) is a global public health problem, and the rapid rise in AMR attributed to inappropriate and/or overuse of antibiotics. Therefore, alternative antimicrobial agents, including those natural origin, are being sought for development new drugs. The purpose our study was analyze chemical composition, antioxidant activities four oregano essential oils (OEOs) from Poland, Europe, Turkey USA. activity (AMA) evaluated using 23 strains, Gram-positive bacteria,...

Rosa rugosa petals are used for production of teas, jams, wines and juices. Despite the wide availability rose cultivars, comprehensive information on petal chemical composition healthful properties is still lacking. Therefore, aim this study was analysis cytotoxic, antioxidant antimicrobial activity petals.Petals R. were evaluated their cytotoxic effect against cervical (HeLa) breast cancer (T47D) cell lines antiradical (with DPPH•). As a result, significant (up to 100% dead cells) (IC₅₀...

Background Chronic lymphocytic leukemia (CLL) leads to significant immune system dysfunction. The predominant clinical presentation in 50% of patients involves recurrent, often severe, infections. Infections are also the most common (60–80%) cause deaths CLL patients. scope infections varies with stage disease. Treatment-naive typically present respiratory tract caused by encapsulated bacteria Streptococcus pneumoniae and Haemophilus influenzae. Since 2012, 13-valent pneumococcal conjugate...

The anti-H. pylori properties of 26 different commercial essential oils were examined in vitro by MIC (minimal inhibitory concentration) determination for the reference strain H. ATCC 43504. We selected 9 with anti-Helicobacter activities and established their phytochemical composition urease inhibition activities. Phytochemical analysis GC-MS method antioxidant activity performed. phenol red was used to screen effect on expressed as IC50 (the half maximal concentration). most active oils, =...

Recently, the occurrence of candidiasis has increased dramatically, especially in immunocompromised patients. Additionally, their treatment is often ineffective due to resistance yeasts antimycotics. Therefore, there a need search for new antifungals. A series nine newly synthesized thiazole derivatives containing cyclopropane system, showing promising activity against Candida spp., been further investigated. We decided verify antifungal towards clinical albicans isolated from oral cavity...

In this study, microbial diversity of the root canal microbiota related to different endodontic infections was investigated. total, 45 patients with endo–perio lesions (8 patients), chronic periapical periodontitis (29 patients) and pulp necrosis were recruited. 19 (42.2%) there secondary infection canals. Microbial specimens collected from canals non-vital teeth or without changes in area visible X-ray. Then, oral detected identified using culture method real-time PCR amplification primers...

Summary Background The impact of long‐term acid suppression on the gastric mucosa remains controversial. Aim To report further observations an established cohort patients with gastro‐oesophageal reflux disease, after 7 years follow‐up. Methods Of original randomized to either antireflux surgery or omeprazole, 117 and 98 remained in medical surgical arms, respectively. Gastric biopsies were taken at baseline throughout study. Results Fifty‐three 39 omeprazole‐treated had Helicobacter pylori...

Quantitative determination of polysaccharides in <em>Ganoderma lucidum </em>fruit bodies from different sawdust cultivation substrates and their antibacterial activity was done. Thirty six samples were analyzed. Four strains </em>(GL01, GL02, GL03 GL04) cultivated on the growth three types: birch (Bo), maple (Kl) or alder (Ol) amended with wheat bran concentrations: 10, 20 30% (w/w). Even though richest GL01 strain, highest yields determined GL04Kl3 sample 112.82 mg/g dry...

Abstract The purpose of this research was to investigate the chemical composition essential oils (EOs) from: Origanum vulgare L., Satureja hortensis Thymus serpyllum L. and vulgaris (Lamiaceae) cultivated in Poland, study their antifungal activity towards clinical isolates oral Candida spp. hydrodistilled were analyzed using GC-MS method. evaluated vitro against reference strains albicans C. glabrata , broth microdilution method according European Committee on Antimicrobial Susceptibility...

The antimicrobial properties of essential oil from Lavandula sp. raise hopes related to its use in phytotherapy. This study aimed evaluate the activity oils cultivars L. angustifolia (‘Hidcote Blue Strain’, ‘Hidcote Blue’) and × intermedia (‘Phenomenal’, ‘Grosso’) grown central-eastern Poland, that is, at border northern lavender cultivation range. chemical composition was determined by GC/MS. Essential concentrations (20, 10, 5, 2.5, 1.25, 0.6, 0.3, 0.16, 0.08, 0.04 mg/mL) were tested...

The occurrence of candidiasis, including superficial infections, has recently increased dramatically, especially in immunocompromised patients. Their treatment is often ineffective due to the resistance yeasts antimycotics. Therefore, there a need search for new antifungals. aim this study was determine antifungal effect clove essential oil (CEO) and eugenol (EUG) towards both reference clinical Candida spp. strains isolated from oral cavity patients with hematological malignancies,...

Abstract The study was designed to determine the total phenolic, flavonoid, o-dihydroxyphenol, tannin, and carotenoid content as well antiradical, antitumor antimicrobial properties of two types galenic preparations from Rosa rugosa Thunb. Such extracts obtained various plant parts have not been studied date. Our findings revealed high antiradical activity examined preparations, with root, leaf flower (IC50 ranging 0.27 0.19 mg dry extract per DPPH·) showing greatest potential. MIC MBC...

This study presents the synthesis and spectral analysis of new derivatives 1,2,4-triazole-3-thione 1,3,4-thiadiazole. New compounds were prepared by cyclization reaction acyl thiosemicarbazide in presence alkaline acidic media. All synthesized screened for their vitro antibacterial activity using agar dilution technique. Six had potential against Gram-positive bacteria (minimal inhibitory concentration [MIC] = 15.63-500 μg/mL). Some showed good especially Bacillus subtilis ATCC 6633 (MIC...