A5075280206- Phytoplasmas and Hemiptera pathogens
- Plant Virus Research Studies
- Cocoa and Sweet Potato Agronomy
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Insect symbiosis and bacterial influences
- CRISPR and Genetic Engineering
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Mosquito-borne diseases and control
- Microgrid Control and Optimization
- Hybrid Renewable Energy Systems
- Virology and Viral Diseases
- Plant Molecular Biology Research
- Phytochemistry and biological activities of Ficus species
- Plant Reproductive Biology
- Ecology and Conservation Studies
- Smart Grid Energy Management
- Plant pathogens and resistance mechanisms
- Genetic and Environmental Crop Studies
- Insect-Plant Interactions and Control
- Plant and Fungal Interactions Research
The University of Tokyo
2016-2024
Abstract Plant pathogenic bacteria have developed effectors to manipulate host cell functions facilitate infection. A certain number of use the conserved ubiquitin–proteasome system in eukaryotic proteolyze targets. The proteasome utilization mechanism is mainly mediated by ubiquitin interaction with target proteins destined for degradation. Phyllogens are a family protein produced phytoplasmas that transform flowers into leaves diverse plants. Here, we present noncanonical phyllogen action...
ABCE-class MADS domain transcription factors (MTFs) are key regulators of floral organ development in angiosperms. Aberrant expression these genes can result abnormal traits such as phyllody. Phyllogen is a virulence factor conserved phytoplasmas, plant pathogenic bacteria the class Mollicutes. It triggers phyllody Arabidopsis thaliana by inducing degradation A- and E-class MTFs. However, it still unknown whether phyllogen induce plants other than A. thaliana, although phytoplasma-associated...
Summary One of the plant host resistance machineries to viruses is attributed recessive alleles genes encoding critical factors for virus infection. This type resistance, also referred as useful revealing plant–virus interactions and breeding antivirus in crop plants. Therefore, it important identify a novel factor responsible robust viruses. Here, we identified mutant from an ethylmethane sulfonate (EMS)‐mutagenized Arabidopsis population which confers plantago asiatica mosaic (PlAMV, genus...
Bogia coconut syndrome (BCS) is one of the lethal yellowing (LY)-type diseases associated with phytoplasma presence that are seriously threatening cultivation worldwide. It has recently emerged, and rapidly spreading in northern parts island New Guinea. BCS-associated phytoplasmas collected different regions were compared terms 16S rRNA gene sequences, revealing high identity among them represented by strain BCS-BoR. Comparative analysis sequences revealed BCS-BoR shared less than a 97.5 %...
Flower malformation represented by phyllody is a common symptom of phytoplasma infection induced novel family effectors called phyllogens. Despite the accumulation functional and structural phyllogen information, molecular mechanisms have not yet been integrated with their evolutionary aspects due to limited data on homologs across diverse lineages. Here, we developed universal PCR-based approach identify 25 phyllogens related nine "Candidatus Phytoplasma" species, including four species...
Abstract Phytoplasmas are transmitted by insect vectors in a persistent propagative manner; however, detailed movements and multiplication patterns of phytoplasmas within remain elusive. In this study, spatiotemporal dynamics onion yellows (OY) phytoplasma its vector Macrosteles striifrons were investigated immunohistochemistry-based 3D imaging, whole-mount fluorescence staining, real-time quantitative PCR. The results indicated that OY entered the anterior midgut epithelium seven days after...
Abstract One of the important antiviral genetic strategies used in crop breeding is recessive resistance. Two eukaryotic translation initiation factor 4E family genes, eIF4E and eIFiso4E , are most common resistance genes whose absence inhibits infection by plant viruses Potyviridae, Carmovirus Cucumovirus . Here, we show that another gene, nCBP acts as a novel gene Arabidopsis thaliana toward Alpha- Betaflexiviridae We found Plantago asiatica mosaic virus (PlAMV), potexvirus, was delayed...
Abstract As an effective strategy to implement electrical load shifting and encourage the use of alternative renewable energies, such as solar wind generation, energy storage system plays important role in internet smart grid. Compressed air is a promising technique due its efficiency, cleanliness, long life, low cost. This paper reviews CAES technologies seeks demonstrate CAES's models, fundamentals, operating modes, classifications. Application perspectives are described promote...
Plant viruses depend on a number of host factors for successful infection. Deficiency critical confers recessively inherited viral resistance in plants. For example, loss Essential poteXvirus Accumulation 1 (EXA1) Arabidopsis thaliana to potexviruses. However, the molecular mechanism how EXA1 assists potexvirus infection remains largely unknown. Previous studies reported that salicylic acid (SA) pathway is upregulated exa1 mutants, and modulates hypersensitive response-related cell death...
ABSTRACT Plant-pathogenic bacteria cause numerous diseases in host plants and can result serious damage. Timely accurate diagnostic techniques are, therefore, crucial. While advances molecular have led to systems able distinguish known plant pathogens at the species or strain level, covering larger categories are mostly lacking. In this study, a specific universal LAMP-based system was developed for phytoplasmas, large group of insect-borne plant-pathogenic that significant agricultural...
Plant virus movement proteins (MPs) localize to plasmodesmata (PD) facilitate cell-to-cell movement. Numerous studies have suggested that MPs use a pathway either through the ER or plasma membrane (PM). Furthermore, recent reported ER-PM contact sites and PM microdomains, which are subdomains found in PM, involved However, functional relationship of these MP traffic PD has not been described previously. We demonstrate here intracellular trafficking fig mosaic (MPFMV) using live cell imaging,...
Phytoplasmas are plant-pathogenic bacteria that infect many important crops and cause serious economic losses worldwide. However, owing to an inability culture phytoplasmas, screening of antimicrobials on media is difficult. The only being used control phytoplasmas tetracycline-class antibiotics. In this study, we developed accurate efficient method evaluate the effects using in vitro plant-phytoplasma co-culture system. We tested 40 antimicrobials, addition tetracycline, four these...
Phytoplasmas are obligate intracellular parasitic bacteria that infect both plants and insects. We previously identified the sigma factor RpoD-dependent consensus promoter sequence of phytoplasma. However, genome-wide landscape RNA transcripts, including non-coding RNAs (ncRNAs) RpoD-independent elements, was still unknown. In this study, we performed an improved sequencing analysis for identification transcription start sites (TSSs) sequences. constructed cDNA libraries using a random...
The 5'-terminal genomic sequence of Cherry virus A (CVA) has long been unknown. We determined the first complete genome an apricot isolate CVA (7,434 nucleotides [nt]). 5'-untranslated region was 107 nt in length, which 53 longer than those known sequences.
To understand protein function deeply, it is important to identify how interacts physically with its target. Phyllogen a phyllody-inducing effector that the K domain of plant MADS-box transcription factors (MTFs), which followed by proteasome-mediated degradation MTF. Although several amino acid residues phyllogen have been identified as being responsible for interaction, exact interface interaction has not elucidated. In this study, we comprehensively explored based on random mutagenesis...
ABSTRACT Hydrangea ringspot virus (HdRSV) is a plant RNA virus, naturally infecting macrophylla . Here, we report the first genomic sequences of two HdRSV isolates from hydrangea plants in Japan. The overall nucleotide these Japanese were 96.0 to 96.3% identical those known European isolates.
Abstract In 2019, brown root rot symptoms were observed in pea sprouts ( Pisum sativum L.) grown a plant factory Japan. Bacteria isolated from symptomatic plants and confirmed to cause the same inoculation seedlings. Based on multilocus sequence analysis bacteriological characteristics, isolates identified as Pseudomonas species belonging asplenii subgroup, which is most closely related vanderleydeniana . To our knowledge, this first report of bacterial disease P subgroup member that...