A5015752202- Plant Virus Research Studies
- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- Insect symbiosis and bacterial influences
- Plant and Fungal Interactions Research
- Cocoa and Sweet Potato Agronomy
- Plant-Microbe Interactions and Immunity
- Bacteriophages and microbial interactions
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Insect-Plant Interactions and Control
- Mosquito-borne diseases and control
- Plant Reproductive Biology
- Phytochemistry and biological activities of Ficus species
- Advanced biosensing and bioanalysis techniques
- Plant Parasitism and Resistance
- Biosensors and Analytical Detection
- Herpesvirus Infections and Treatments
- Banana Cultivation and Research
- Virology and Viral Diseases
- Chromosomal and Genetic Variations
- Yeasts and Rust Fungi Studies
The University of Tokyo
2014-2023
ABCE-class MADS domain transcription factors (MTFs) are key regulators of floral organ development in angiosperms. Aberrant expression these genes can result abnormal traits such as phyllody. Phyllogen is a virulence factor conserved phytoplasmas, plant pathogenic bacteria the class Mollicutes. It triggers phyllody Arabidopsis thaliana by inducing degradation A- and E-class MTFs. However, it still unknown whether phyllogen induce plants other than A. thaliana, although phytoplasma-associated...
Bogia coconut syndrome (BCS) is one of the lethal yellowing (LY)-type diseases associated with phytoplasma presence that are seriously threatening cultivation worldwide. It has recently emerged, and rapidly spreading in northern parts island New Guinea. BCS-associated phytoplasmas collected different regions were compared terms 16S rRNA gene sequences, revealing high identity among them represented by strain BCS-BoR. Comparative analysis sequences revealed BCS-BoR shared less than a 97.5 %...
Flower malformation represented by phyllody is a common symptom of phytoplasma infection induced novel family effectors called phyllogens. Despite the accumulation functional and structural phyllogen information, molecular mechanisms have not yet been integrated with their evolutionary aspects due to limited data on homologs across diverse lineages. Here, we developed universal PCR-based approach identify 25 phyllogens related nine "Candidatus Phytoplasma" species, including four species...
Abstract Phytoplasmas are transmitted by insect vectors in a persistent propagative manner; however, detailed movements and multiplication patterns of phytoplasmas within remain elusive. In this study, spatiotemporal dynamics onion yellows (OY) phytoplasma its vector Macrosteles striifrons were investigated immunohistochemistry-based 3D imaging, whole-mount fluorescence staining, real-time quantitative PCR. The results indicated that OY entered the anterior midgut epithelium seven days after...
Adhesins are microbial surface proteins that mediate the adherence of pathogens to host cell surfaces. In Mollicutes, several adhesins have been reported in mycoplasmas and spiroplasmas. P40 Mycoplasma agalactiae P89 Spiroplasma citri contain a conserved amino acid sequence known as Mollicutes adhesin motif (MAM), whose function adhesion remains unclear. Here, we show phytoplasmas, which plant-pathogenic mollicutes transmitted by insect vectors, possess an adhesion-containing MAM was...
Phytoplasmas are obligate intracellular plant pathogenic bacteria that can induce phyllody, which is a type of abnormal floral organ development. possess phyllogens, effector proteins cause phyllody in plants. Phylogenetic comparisons phyllogen and 16S rRNA genes have suggested undergo horizontal transfer between phytoplasma species strains. However, the mechanisms evolutionary implications this gene unclear. Here, we analyzed synteny flanking genomic regions from 17 strains were related to...
Phytoplasmas (class, Mollicutes) are insect-transmissible and plant-pathogenic bacteria that multiply intracellularly in both plants insects through host switching. Our previous study revealed phytoplasmal sigma factor rpoD of OY-M strain (rpoDOY) could be a key regulator switching, because the expression level rpoDOY was higher insect hosts than plant hosts. In this study, we developed an vitro transcription assay system to identify RpoDOY-dependent genes consensus promoter elements. The...
Phytoplasmas are plant-pathogenic bacteria that infect many important crops and cause serious economic losses worldwide. However, owing to an inability culture phytoplasmas, screening of antimicrobials on media is difficult. The only being used control phytoplasmas tetracycline-class antibiotics. In this study, we developed accurate efficient method evaluate the effects using in vitro plant-phytoplasma co-culture system. We tested 40 antimicrobials, addition tetracycline, four these...
Regardless of the general model translation in eukaryotic cells, a number studies suggested that many mRNAs encode multiple proteins. Leaky scanning, which supplies ribosomes to downstream open reading frames (ORFs) by readthrough upstream ORFs, has great potential translate polycistronic mRNAs. However, mRNA elements controlling leaky scanning and their biological relevance have rarely been elucidated, with exceptions such as Kozak sequence. Here, we analyzed strategy plant RNA virus three...
We report the complete genome sequence of a Japanese isolate Tea plant necrotic ring blotch virus (TPNRBV-J). The predicted TPNRBV-J genes have same organization as those Chinese isolate, and 5' termini segments conserved nucleotide sequences.
ABSTRACT Alternanthera mosaic virus (AltMV) infecting Achyranthes bidentata was first detected in Asia, and the complete genome sequence (6,604 nucleotides) determined. Sequence identity analysis phylogenetic confirmed that this isolate is most phylogenetically distant AltMV worldwide.
Phytoplasmas are obligate intracellular parasitic bacteria that infect both plants and insects. We previously identified the sigma factor RpoD-dependent consensus promoter sequence of phytoplasma. However, genome-wide landscape RNA transcripts, including non-coding RNAs (ncRNAs) RpoD-independent elements, was still unknown. In this study, we performed an improved sequencing analysis for identification transcription start sites (TSSs) sequences. constructed cDNA libraries using a random...
The 5'-terminal genomic sequence of Cherry virus A (CVA) has long been unknown. We determined the first complete genome an apricot isolate CVA (7,434 nucleotides [nt]). 5'-untranslated region was 107 nt in length, which 53 longer than those known sequences.
The complete genome sequence of the first Japanese isolate carrot torradovirus 1 (CaTV1-J), which infects Angelica keiskei, was determined. This is report a CaTV1 obtained from A. keiskei.
We report here the first complete genome sequence of a Japanese isolate lychnis mottle virus (LycMoV-J). The segments LycMoV-J have unique structure in their 3' untranslated regions, and encoded proteins same as that an reported from South Korea.