A5038970909- Plant Virus Research Studies
- Transgenic Plants and Applications
- Plant and Fungal Interactions Research
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Endoplasmic Reticulum Stress and Disease
- Plant Disease Resistance and Genetics
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Viral Infections and Vectors
- Insect-Plant Interactions and Control
- Autophagy in Disease and Therapy
- Crop Yield and Soil Fertility
- Genetics and Plant Breeding
- Wheat and Barley Genetics and Pathology
- Plant Molecular Biology Research
- Animal Virus Infections Studies
- Photosynthetic Processes and Mechanisms
- Plant Disease Management Techniques
- Plant nutrient uptake and metabolism
- Polyomavirus and related diseases
- Toxin Mechanisms and Immunotoxins
- Plant pathogens and resistance mechanisms
- Plant Reproductive Biology
Texas A&M University
2011-2025
Mitchell Institute
2017-2024
Research Institute of Dallas
2020
Texas A&M University System
2020
Oklahoma State University Oklahoma City
2004-2019
Oklahoma State University
2008-2017
Institute of Entomology
2012
Plant (United States)
2012
Iowa State University
2011
GTx (United States)
2000-2008
Transport of viruses from cell to in plants typically involves one or more viral proteins that supply dedicated movement functions. leaf through phloem, long-distance transport, is a poorly understood process with requirements differing those cell-to-cell movement. Through genetic analysis tobacco etch virus (TEV; potyvirus group), novel factor was identified facilitates vascular-associated tobacco. A mutation the central region helper component proteinase (HC-Pro), TEV-encoded protein...
Infection with Potato virus X (PVX) in Nicotiana benthamiana plants leads to increased transcript levels of several stress-related host genes, including basic-region leucine zipper 60 (bZIP60), SKP1, ER luminal binding protein (BiP), disulfide isomerase (PDI), calreticulin (CRT), and calmodulin (CAM). bZIP60 is a key transcription factor that responds endoplasmic reticulum (ER) stress induces the expression ER-resident chaperones (BiP, PDI, CRT, CAM). SKP1 component SCF (for SKP1-Cullin-F...
Abstract Background Post transcriptional gene silencing (PTGS) is a mechanism harnessed by plant biologists to knock down expression. siRNAs contribute PTGS that are synthesized from mRNAs or viral RNAs and function guide cellular endoribonucleases target for degradation. Plant have employed electroporation deliver artificial protoplasts study expression mechanisms at the single cell level. One drawback of extensive loss viable occurs as result transfection technology. Results We fluorescent...
The unfolded protein response (UPR) signaling network encompasses two pathways in plants, one mediated by inositol-requiring protein-1 (IRE1)-bZIP60 mRNA and the other site-1/site-2 proteases (S1P/S2P)-bZIP17/bZIP28. As major sensor of UPR eukaryotes, IRE1, to endoplasmic reticulum (ER) stress, catalyzes unconventional splicing HAC1 yeast, bZIP60 plants XBP1 metazoans. Recent studies suggest that IRE1p mRNA, only pathway found evolves as a cognate system responsible for robust induction....
Abstract The green fluorescent protein (GFP) gene was fused to the potato virus X (PVX) TGBp2 gene, inserted into either PVX infectious clone or pRTL2 plasmids, and used study subcellular targeting. In protoplasts plants inoculated with PVX-GFP:TGBp2 transfected pRTL2-GFP:TGBp2, fluorescence mainly in vesicles endoplasmic reticulum (ER). During late stages of infection, became increasingly cytosolic nuclear. Protoplasts pRTL2-GFP:TGBp2 were treated cycloheximide decline GFP greater...
The tobacco etch potyvirus (TEV) polyprotein is proteolytically processed by three viral proteinases (NIa, HC-Pro, and P1). While the NIa HC-Pro each provide multiple functions essential for infectivity, role of P1 proteinase beyond its autoproteolytic activity understood poorly. To determine if necessary genome amplification and/or virus movement from cell to cell, a mutant lacking entire coding region (delta mutant) was produced with modified TEV strain (TEV-GUS) expressing...
Cellular chaperones and folding enzymes play central roles in the formation of positive strand negative RNA virus infection. This article examines key cellular discusses evidence that these factors are diverted from their functions to alternative For most discussed, primary role cell is ensure protein quality control. They system components drive substrate folding, complex assembly or disaggregation. Their activities often depend upon co-chaperones ATP hydrolysis. During plant infection,...
This article demonstrates the encapsulation of cubic iron oxide nanoparticles (NPs) by Brome mosaic virus capsid shells and formation, for first time, virus-based (VNPs) with cores. Cubic NPs functionalized phospholipids containing poly(ethylene glycol) tails terminal carboxyl groups exhibited exceptional relaxivity in magnetic resonance imaging experiments, which opens way vivo MRI studies systemic movement plants. Preliminary data on cell-to-cell long-distance transit behavior VNPs...
Abstract Background Salicylic acid (SA) regulates multiple anti-viral mechanisms, including mechanism(s) that may be negatively regulated by the mitochondrial enzyme, alternative oxidase (AOX), sole component of respiratory pathway. However, studies this mechanism can confounded SA-mediated induction RNA-dependent RNA polymerase 1, a antiviral silencing We made transgenic Nicotiana benthamiana plants in which pathway capacity was either increased constitutive expression AOX, or decreased...
The inositol requiring enzyme (IRE1) is an endoplasmic reticulum (ER) stress sensor. When activated, it splices the bZIP60 mRNA, producing a truncated transcription factor that upregulates genes involved in unfolded protein response. Bax inhibitor 1 (BI-1) another ER sensor regulates cell death response to environmental assaults. potyvirus 6K2 and potexvirus TGB3 proteins are known reside ER, serving, respectively, as anchors for viral replicase movement complex. This study used green...