A5024568632- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Sociopolitical Dynamics in Russia
- Virus-based gene therapy research
- Regional Socio-Economic Development Trends
- Advanced biosensing and bioanalysis techniques
- Educational Innovations and Challenges
- Innovations in Education and Learning Technologies
- Advanced Fluorescence Microscopy Techniques
- Nanomaterials for catalytic reactions
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Metal-Organic Frameworks: Synthesis and Applications
- Foreign Language Teaching Methods
- Educational Methods and Teacher Development
- Labor market dynamics and wage inequality
- Discourse Analysis and Cultural Communication
- Regional Economic Development and Innovation
- Business and Economic Development
- Force Microscopy Techniques and Applications
- Mesoporous Materials and Catalysis
- Advanced Biosensing Techniques and Applications
- Education Systems and Policy
- Viral Infections and Immunology Research
- Polymer Surface Interaction Studies
Indiana University Bloomington
2013-2025
Indiana University
2025
Lomonosov Moscow State University
2005-2024
Institute of Chemistry of Silicates named after I.V. Grebenshchikov
2024
Sarov Institute of Physics and Technology
2024
Togliatti State University
2014-2023
Moscow Region State University
2020
Angel Kanchev University of Ruse
2018
Indiana University School of Medicine
2016
Indiana University – Purdue University Indianapolis
2016
Efficient encapsulation of functionalized spherical nanoparticles by viral protein cages was found to occur even if the nanoparticle is larger than inner cavity native capsid. This result raises intriguing possibility reprogramming self-assembly structural proteins. The iron oxide nanotemplates used in this work are superparamagnetic, with a blocking temperature about 250 K, making these virus-like particles interesting for applications such as magnetic resonance imaging and biomagnetic...
Abstract Circulating endothelial microparticles (EMPs) are emerging as biomarkers of chronic obstructive pulmonary disease (COPD) in individuals exposed to cigarette smoke (CS), but their mechanism release and function remain unknown. We assessed biochemical functional characteristics EMPs circulating (cMPs) released by CS. CS exposure was sufficient increase microparticle levels plasma humans mice supernatants primary human lung microvascular cells. CS-released contained predominantly...
Self-assembling icosahedral protein cages have potencially useful physical and chemical characteristics for a variety of nanotechnology applications, ranging from therapeutic or diagnostic vectors to building blocks hierarchical materials. For application-specific functional control cage assemblies, deeper understanding the interaction between its payload is necessary. Protein-cage encapsulated nanoparticles, with their well-defined surface chemistry, allow systematic over key parameters...
This article demonstrates the encapsulation of cubic iron oxide nanoparticles (NPs) by Brome mosaic virus capsid shells and formation, for first time, virus-based (VNPs) with cores. Cubic NPs functionalized phospholipids containing poly(ethylene glycol) tails terminal carboxyl groups exhibited exceptional relaxivity in magnetic resonance imaging experiments, which opens way vivo MRI studies systemic movement plants. Preliminary data on cell-to-cell long-distance transit behavior VNPs...
Variance in the properties of optical mesoscopic probes is often a limiting factor applications. In thermodynamic limit, smaller probe, larger relative variance. However, specific viral protein cages can assemble efficiently outside bounds statistical fluctuations at equilibrium through process that characterized by intrinsic quality-control and self-limiting capabilities. this paper, an approach described leverages stoichiometric structural accuracy murine polyoma virus capsid assembly to...
We studied the friction dynamics of icosahedral viruses adsorbed to solid surfaces probe their adhesion. Using lateral torsion cantilevers in atomic force microscopy move individual capsids a liquid environment, we found that virions tend roll rather than slide on surface. In contrast, rigid, ligand-stabilized gold nanoparticles are more likely combine rolling with sliding under same conditions. The experiments indicate required drag surface is four times less AuNPs, while work needed induce...
Viral nanoparticles (VNPs) are becoming versatile tools in platform technology development. Their well-defined structures as well their programmability through chemical and genetic modification allow VNPs to be engineered for potential imaging therapeutic applications. In this article, we report the application of a variety bioconjugation chemistries plant VNP Brome mosaic virus (BMV). Functional BMV displaying multiple copies fluorescent dyes, PEG molecules, chemotherapeutic drug moieties,...
Since the concept was first introduced by Brian Chait and co-workers in 1991, mass spectrometry of proteins protein complexes under non-denaturing conditions (native MS) has strongly developed, through parallel advances instrumentation, sample preparation, data analysis tools. However, success rate native MS analysis, particularly heterogeneous mega-Dalton (MDa) complexes, still depends on careful instrument modification. Here, we further explore these boundaries spectrometry, analyzing two...
Viral nanoparticles used for biomedical applications must be able to discriminate between tumor or virus-infected host cells and healthy cells. In addition, viral have the flexibility incorporate a wide range of cargo, from inorganic metals mRNAs small molecules. Alphaviruses are family enveloped viruses which some species intrinsically capable systemic targeting. Alphavirus virus-like particles, nanoparticles, can generated in vitro self-assembled core-like particles using nonviral nucleic...
Nanoparticles can be encapsulated by virus coat proteins if their surfaces are functionalized to acquire a sufficiently large negative charge. A minimal surface charge is required overcome (i) repulsive interactions between the positively charged RNA-binding domains on and (ii) loss of mixing translational entropy RNA capsid proteins. Here, we present model describing encapsulation spherical particles bearing weakly acidic groups investigate how regulation size polydispersity impact upon...
A metal–organic molecular net composed of tannic acid (TA) and iron(III) was constructed around the brome mosaic virus (BMV) particle to determine whether added could act as a transport barrier for water, if stabilize in physically or chemically challenging environments. This new engineering strategy is expected provide benefits both study technological applications viruses. For instance, wrapped thin layer be extracted from solution either air vacuum, its structure, composition even...
Abstract Non‐enveloped RNA viruses pervade all domains of life. In a cell, they co‐assemble from viral and capsid proteins. Virus‐like particles can form in vitro where virtually any non‐cognate polyanionic cargo be packaged. How only gets selected for packaging vivo, presence myriad other species, has been puzzle. Through combination charge detection mass spectrometry cryo‐electron microscopy, it is determined that co‐assembling brome mosaic virus (BMV) coat proteins nucleic acid oligomers...
In this paper for the first time we present a systematic study of influence hydrophobicity Pd(II) compounds, (CH3CN)2PdCl2, (PhCN)2PdCl2, (Sty)(CH3CN)PdCl2, and (StyPdCl2)2, on nanoparticle (NP) formation in pores hydrophobic micro/mesoporous hypercrosslinked polystyrene (HPS). The morphology composition HPS–Pd nanocomposites were studied using transmission electron microscopy, X-ray fluorescence measurements, photoelectron spectroscopy, liquid nitrogen physisorption. size location Pd...
Capsid disassembly and genome release are critical steps in the lifecycle of a virus. However, their mechanisms poorly understood, both vivo vitro. Here, we have identified two vitro pathways brome mosaic virus (BMV) by charge detection mass spectrometry transmission electron microscopy. When subjected to pH jump basic environment at low ionic strength, protein–RNA interactions disrupted. Under these conditions, BMV appears disassemble mainly through global cleavage event into main...
Brome mosaic virus (BMV) packages its genomic RNAs (RNA1, RNA2, and RNA3) subgenomic RNA4 into three different particles. However, since the in virions have distinct lengths electrostatic charges, we hypothesize that subsets of should properties. A glutamine to cysteine substitution at position 120 capsid protein (CP) was found result a mutant named QC exhibited dramatically altered ratio virions. RNA2 far more abundant than other RNAs, although ratios could be affected by host plant...
Assembly pathways of virus-like particles formed from a virus coat protein cage encapsulating metal nanoparticle were studied by intrinsic fluorescence quenching. Depending on buffer conditions, the in vitro formation particle can take place through cooperative or non-cooperative adsorption subunits template. Simple equilibrium models provided estimates for thermodynamic forces driving assembly as well size critical nucleus case growth.
Virus coat proteins of small isometric plant viruses readily assemble into symmetric, icosahedral cages encapsulating noncognate cargo, provided the cargo meets a minimal set chemical and physical requirements. While this capability has been intensely explored for certain virus-enabled nanotechnologies, additional applications require lower symmetry than that an icosahedron. Here, we show virus can efficiently around metal nanorods spherocylindrical closed shells with hexagonally...
It has been just over 100 years since inventor Joseph Coyle perfected the egg carton-a package format that known very little changes its first appearance ( Dhillon , S. B. C. Inventor Created Better Way to Carry Eggs. In The Globe and Mail Vancouver 2013 ). this article, we extend Coyle's old idea study of mechanical properties viruses. Virus stiffness, strength, breaking force obtained by spectroscopy atomic microscopy (AFM) provide knowledge required for designing nanocontainers...
The presented study of the PM10 concentrations in atmosphere ground layer emitted from industrial sources Industrial zone city Ruse days with exceedances for this pollutant is aimed to determining possibility cross-border BG-RO contamination. Climatic conditions measured these January 2017 are also taken into account Jet Gauss model and Langrageo-statistical approach describing turbulent diffusion. results show that investigated reaching territory Republic Romania low have no potential...
The strong configuration dependence of collective surface plasmon resonances in an array metal nanoparticles provides opportunity to develop a bioinspired tool for sensing mechanical deformations soft matter at the nanoscale. We study feasibility strain sensor based on icosahedral encapsulated by virus capsid. When system undergoes deformation, optical scattering cross-section spectra as well induced electric field profile change. By numerical simulations, we examine how these changes depend...