A5050225630- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Bone Metabolism and Diseases
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Inflammatory mediators and NSAID effects
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
- Vaccine Coverage and Hesitancy
- Influenza Virus Research Studies
- Osteoarthritis Treatment and Mechanisms
- Immune cells in cancer
- Child and Adolescent Psychosocial and Emotional Development
- SARS-CoV-2 and COVID-19 Research
- Glycosylation and Glycoproteins Research
- Antimicrobial Peptides and Activities
- Viral Infections and Outbreaks Research
- Hepatitis B Virus Studies
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Behavioral and Psychological Studies
- Eicosanoids and Hypertension Pharmacology
- Child and Animal Learning Development
- Biosensors and Analytical Detection
- Cancer, Stress, Anesthesia, and Immune Response
The University of Western Australia
2005-2024
Canadian Nuclear Laboratories
2021
World Health Organization
2006-2015
Appalachian State University
2015
World Health Organization - Pakistan
2006
College of the Sequoias
1997
Merck & Co., Inc., Rahway, NJ, USA (United States)
1977-1990
Princeton University
1984-1989
Miles College
1989
University of Sheffield
1983-1985
Interleukin-1 (IL-1) is a macrophage derived mediator whose properties suggest that it could play role in the pathology of arthritis. To test this hypothesis, joint fluids from patients with serveral different arthritides were tested. Small amounts IL-1-like activity recovered many these after affinity chromatography over column rabbit anti-human IL-1. Positive obtained rheumatoid arthritis, psoriatic Reiter's syndrome, osteoarthritis, gout, and traumatic Upon gel filtration, factor...
Abstract Interleukin 1 (IL 1) was shown to enhance in vivo secondary antibody responses of mice a protein antigen. The activity found be dose and time dependent. maximum enhancing effect obtained with 2000 LAF units IL given 2 hr after the priming observation is consistent vitro immunoenhancing activities strongly suggests that this may an important mediator host defense responses, as well stimulatory effects some adjuvants.
The process of induction bone formation, which follows resorption during normal and pathological turnover, is well documented. However, the mechanisms responsible for this are unclear. Mononuclear phagocytes present at sites remodeling could play a role in "coupling" formation to resorption. This study was designed investigate such possibility. By measuring both increase [3H]thymidine incorporation cell number, we found that human monocytes culture released factors capable stimulating...
With the Ebola epidemic in West Africa continuing to grow, World Health Organization convened an urgent meeting on September 29 and 30 assess efforts under way evaluate produce safe effective vaccines as soon possible.
Abstract During the enzymatic disaggregation of human synovium, used in process isolating synoviocytes, a factor was liberated into culture medium that exhibited thymocyte mitogenic properties interleukin‐1. Like interleukin‐1, this synovial‐derived mitogen could be isolated using an affinity column antihuman leukocytic pyrogen. By gel filtration and isoelectric focusing, cofractionated with monocyte‐derived Finally, shown to exhibit other interleukin‐1: stimulation secretion interleukin‐2,...
Abstract Monocytes have been shown to secrete factors which stimulate the destruction of cartilage. Since one monocyte products, interleukin‐1 (IL‐1), has release collagenase and prostaglandin E from synoviocytes, we investigated whether IL‐1 is also responsible for chondrocyte activation. Purified preparations derived human blood monocytes stimulated production plasminogen activator by articular chondrocytes. After Sephadex G‐75 chromatography, lymphocyte‐activating chondrocyteactivating...
The supernatants of human monocytes incubated with endotoxin are able to stimulate the proliferation murine thymocytes in presence PHA. This is known as LAF (lymphocyte activating factor) activity and a characteristic interleukin 1 (IL 1). can be resolved into four major fractions: 15,000 dalton (pI 7), 5.5), 35,000 5.5) fraction. To determine whether these fractions shared other biologic activities ascribed IL 1, they were compared series bioassays. When standardized respect their...
Abstract The humoral response of thymus-derived (T) cell-depleted murine spleen cultures was stimulated by the addition human leukocytes. stimulatory cell type found to be adherent and phagocytic suggesting that it is monocyte. stimulation appears mediated a factor released cells into culture medium.
Experimental evidence from many sources indicates that mitochondria are semi- autonomous organelles containing DNA, which probably serves as a genetic determinant for some aspects of mitochondrial function.'In Neurospora crassa, have been shown to DNA with pattern replication' and buoyant density3 different nuclear DNA..In addition, DNTA-dependent RNA polymerase special properties3 transfer RNA's (tRNA) amino acid acceptor function4 demonstrated in these organelles.One tRNA's was found...
Abstract Tendinopathy is one of the most common musculoskeletal diseases, and mechanical overload considered its primary cause. However, underlying mechanism through which induces tendinopathy has not been determined. In this study, we identified for first time that tendon cells can release extracellular mitochondria (ExtraMito) particles, a subtype medium particles (mEPs), into environment process regulated by loading. RNA sequencing systematically revealed oxygen-related reactions,...
Interleukin 1 enhances proliferation of murine thymocytes in the presence lectins, and is also known to stimulate release prostaglandins neutral proteases from a variety cell types. We have previously shown that factor isolated culture media disaggregated lining cells human synovial membrane was indistinguishable monocyte-derived interleukin 1. report here either source stimulates hyaluronate synthesis by cells. Upon gel filtration or isoelectric focusing superna-tants,...
The ability of steroids to modulate the appearance Interleukin-1(IL-1) in vivo was evaluated a model endotoxin shock. High levels IL-1 were found serum from A/J mice which sensitized with P. acnes and challenged bacterial lipopolysaccharide (LPS). factor appeared 2-4 hours after LPS challenge dependent on period sensitization dose LPS. Treating dexamethasone prior resulted significantly lower thymocyte proliferative activity serum. Three experiments demonstrated that this reduced reflects...
Human monocytes, when appropriately stimulated in vitro, release into the culture medium a factor (BAF) that stimulates IgM response of T-depleted murine splenocytes to heterologous erythrocytes. The behavior this on gel filtration, isoelectric focusing, ion exchange chromatography, and isopycnic centrifugation was studied. BAF appears be molecule 15,000 daltons, pI 6.5, 1.33 g/ml with low solubility at ionic strength. It is stable acid, mild heating, long-term storage. Activity lost alkali...
Abstract The function of macrophages in the development primary plaque-forming cell responses cultures mouse splenic lymphoid cells has been investigated. Splenic and peritoneal exudate are equally efficient supporting by cells. active population is a phagocytic macrophage. Macrophages have at least two distinct functions. One to promote viability or survival culture; this can be duplicated reducing agent 2-mercaptoethanol. second antigen presentation.
Background: Virtual teams collaborate across distances using information communication technologies (ICTs). A distinctive set of skills is needed by people who work successfully in virtual teams, and few universities or companies provide structured education training teamwork. At a midsized southeastern Masters Comprehensive University, professors from the Colleges Arts Sciences, Business, Education came together to explore how they might use cross-disciplinary student (groups comprised...
Abstract Human monocytes have been reported to release factors that can elicit distinct responses from a number of different target cells. Three these activities are compared in this report. Supernatants endotoxin-stimulated shown stimulate the direct plaque response T cell deficient splenocytes vitro (BAF assay), thymidine incorporation by thymocytes (mitogen and synergize with phytohemagglutinin stimulation (PHA-enhancing assay). By gel filtration, stimulating activity was recovered as...