A5081228617- CAR-T cell therapy research
- Child Nutrition and Feeding Issues
- Autism Spectrum Disorder Research
- Infant Development and Preterm Care
- Cancer Cells and Metastasis
- Advancements in Semiconductor Devices and Circuit Design
- Family and Disability Support Research
- Immune Cell Function and Interaction
- Congenital Heart Disease Studies
- Silicon Carbide Semiconductor Technologies
- Adenosine and Purinergic Signaling
- Biosimilars and Bioanalytical Methods
- Viral Infectious Diseases and Gene Expression in Insects
- Cystic Fibrosis Research Advances
- Health Education and Validation
- Developmental and Educational Neuropsychology
- Education during COVID-19 pandemic
- Microfluidic and Bio-sensing Technologies
- Williams Syndrome Research
- Coronary Artery Anomalies
- Neonatal Respiratory Health Research
- Cancer Research and Treatments
- Telemedicine and Telehealth Implementation
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
Garrahan Hospital
2006-2025
The University of Adelaide
2023-2024
Women's and Children's Health Network
2022
Women's and Children's Hospital
2021
Chimeric antigen receptor (CAR)-T cell immunotherapy is a novel treatment that genetically modifies the patients' own T cells to target and kill malignant cells. However, identification of tumour-specific antigens expressed on multiple solid cancer types, remains major challenge. P2X purinoceptor 7 (P2X7) surface ATP gated cation channel, dysfunctional version P2X7, named nfP2X7, has been identified from tissues, while being undetectable healthy We present prototype -human CAR-T construct...
Abstract Objectives Recent studies have identified expression of the non‐functional P2X7 (nfP2X7) receptor on various malignant cells including ovarian cancer, but not normal cells, which makes it a promising tumour‐associated antigen candidate for chimeric (CAR)‐T‐cell immunotherapies. In this study, we assessed cytotoxic effects nfP2X7‐CAR‐T cancer using in vitro and vivo models. Methods We evaluated cell lines (SKOV‐3, OVCAR3, OVCAR5), peritoneal (LP‐9) primary serous derived from patient...
Autism spectrum disorder (ASD) affects the daily functioning of children and their families; however, in Argentina, there are no standardized tools to guide description, evaluation, follow-up disability with ASD. To fill this gap, overarching purpose study was create a novel tool guided by International Classification Functioning, Disability, Health (ICF) Core Sets for ASD clinical practice.
Chimeric antigen receptor T (CAR-T) cell therapy is rapidly becoming a frontline cancer therapy. However, the manufacturing process time-, labor- and cost-intensive, it suffers from significant bottlenecks. Many CAR-T products fail to reach viability release criteria set by regulators for commercial products. This results in non-recoupable costs manufacturer detrimental patients who may not receive their scheduled treatment or out-of-specification suboptimal formulation. It demonstrated here...
Abstract Colorectal cancer (CRC) is the third most diagnosed and incidence rates are rapidly increasing among young adults. Despite current treatments, 5-year survival rate approximately 65%, highlighting an urgent need for new therapies. Leucine-rich repeat-containing G-protein coupled receptor (LGR5) a promising antigen target chimeric (CAR)-T cell therapy, which may represent novel effective treatment CRC. LGR5 binds R-spondins functions to enhance Wnt β-catenin signalling involved in...
Sleep problems (SPs) are common, especially among children with developmental disorders (DDs), and affect their functioning quality of family life. Pediatricians play a major role in management.To define the frequency types SPs sample DDs, determine proportion pediatricians who addressed such difficulties, assess effects sleep hygiene (SH), describe impact COVID-19 pandemic on sleep.This was quasiexperiment. were diagnosed based parents' concerns and/or clinical criteria. SH strategies...
Abstract Colorectal cancer is the third most diagnosed worldwide, and responsible for second largest number of related deaths, warranting need new treatments. The successful use chimeric antigen receptor T (CAR-T) cells against B-cell malignancies has inspired search CAR-T cell therapies solid tumours such as colorectal cancer. Cancer stem (CSC) are a small population within tumour with capacity to self-renew, differentiate, initiate tumours. CSC resistant chemotherapy radiotherapy enriched...
Abstract Incidences of early-onset colorectal cancer (CRC) have been accelerating worldwide. To begin to address the lack therapeutic options for CRC, we developed a CAR-T cell therapy specific leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5). LGR5 is known potentiate Wnt/Β-catenin signaling and mark cells-of-origin in intestinal cancers. Extensive screening has demonstrated elevated expression which correlated with reduced patient survival. In intimately linked metastasis,...
Introduction.Autism spectrum disorder (ASD) is characterized by difficulties in social communication and repetitive stereotyped behaviors.In addition to the diagnostic category, activities performed children adolescents their involvement are main aspects be considered according International Classification of Functioning, Disability, Health (ICF) proposed World Organization describe health status.In a previous study, we developed first version pediatric tool based on ICF called ICF-ASD for...
Teleconsultation is one of the components telemedicine. Developmental pediatricians provide assessments and followup to children with developmental challenges. During pandemic, due movement travel restrictions importance continued care, started working remotely virtual consultations. The objective this study was describe implementation characteristics patients seen, their families' perception. A total 122 teleconsultations were scheduled. Patients' mean age 40 ± 13 months. most common...
Abstract While CAR-T cells have demonstrated significant efficacy in the treatment of haematological malignancies, there are many challenges to overcome developing cell therapies against solid tumours. In particular, scarcity recognised effective target antigens tumours remains a hurdle. Leucine-rich repeat-containing G-protein coupled receptor (LGR5) is cancer stem marker, which mediates important roles tumor initiation and metastasis. Upregulated LGR5 expression malignant contexts...
Abstract Leucine-rich repeat containing G-protein coupled receptor (LGR5) is stem cell marker that potentiates canonical signaling in the Wnt/β-catenin pathway (Barker et al. 2007). LGR5 known to be expressed on several tissues; particularly cells within intestinal crypts and niche at base of hair follicles 2007 Jaks 2008). The plasticity phenotype LGR5+ has led their identification as a cancer for some solid tumors including colorectal (CRC) 2009). This presents potential target molecule...
Abstract The measurement of chimeric antigen receptor (CAR) expression is critical for the development, qualification and quantitation CAR-T cells clinical applications. There a comprehensive range tools commercially available specifically measuring CAR on CD19-targeting cells. However, with rapidly expanding repertoire in development targeting diverse tumour antigens, there paucity readily implementable assays that measure expression, which essential translation into clinic. We have...
Abstract Chimeric antigen receptor (CAR) T cells have demonstrated robust clinical efficacy against B-cell malignancies, and rapid translation to approval for use in man has inspired the search CAR-T solid tumours. Cancer stem are a small population of within tumour that capacity self-renew, differentiate, initiate new known be resistant chemotherapy radiotherapy enriched residual disease, which allows relapse. Targeting cancer is desirable as it will reduce tumours’ ability generate cells,...
Objectives: Describe the development of prematures at age 18 to 24 months, enrolled Service Clinicas Interdisciplinarias Garrahan Hospital and characterize those with cognitive delay comorbilities.
Abstract Chimeric antigen receptor (CAR)-T immunotherapy is a novel treatment that genetically modifies the patient’s own T cells to target and kill malignant cells. CAR-T demonstrated robust clinical activity against certain B-cell malignancies. However, identification of tumour-specific antigens expressed on multiple cancer types, especially solid cancers, remains major challenge. P2X purinoceptor 7 (P2X7) an ATP gated cation channel forms homotrimers heterotrimers at cell surface. When...