A5041816097- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Chemical Synthesis and Analysis
- RNA and protein synthesis mechanisms
- Chemistry and Chemical Engineering
- Steroid Chemistry and Biochemistry
- Microbial Community Ecology and Physiology
John Innes Centre
2020-2022
Norwich Research Park
2020-2021
The formicamycins are promising antibiotics first identified in Streptomyces formicae KY5, which produces the compounds at low levels. Here, we show that by understanding regulation of for biosynthetic gene cluster (BGC), can rewire BGC to increase production consists 24 genes expressed on nine transcripts. MarR regulator ForJ represses expression seven transcripts encoding major as well ForGF two-component system initiates biosynthesis. We overexpression forGF a ΔforJ background increases...
Streptomyces bacteria make diverse specialised metabolites that form the basis of ~55% clinically used antibiotics. Despite this, only 3% their encoded have been matched to molecules and understanding how biosynthesis is controlled essential fully exploit potential. Here we use formicae formicamycin biosynthetic pathway as a model understand complex regulation metabolism. We analysed all three pathway-specific regulators found subject negative feedback redox control via two MarR-family...
Actinobacteria is an ancient phylum of Gram-positive bacteria with a characteristic high GC content to their DNA. The ActinoBase Wiki focused on the filamentous actinobacteria, such as Streptomyces species, and techniques growth conditions used study them. These organisms are studied because complex developmental life cycles diverse specialised metabolism which produces many antibiotics currently in clinic. community effort that provides valuable freely accessible resources, including...
Abstract The formicamycins are promising antibiotics with potent activity against Gram-positive pathogens including VRE and MRSA display a high barrier to selection of resistant isolates. They were first identified in Streptomyces formicae KY5, which produces the at low levels on solid agar but not liquid culture, thus hindering further investigation these antibacterial compounds. We hypothesised that by understanding organisation regulation for biosynthetic gene cluster, we could rationally...