A5013596712- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Microbial Community Ecology and Physiology
- Fungal and yeast genetics research
- Legume Nitrogen Fixing Symbiosis
- Probiotics and Fermented Foods
- Plant Pathogens and Fungal Diseases
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Microbial Natural Products and Biosynthesis
- Preterm Birth and Chorioamnionitis
- Mycorrhizal Fungi and Plant Interactions
- Biofuel production and bioconversion
- Gut microbiota and health
- Nematode management and characterization studies
- Endoplasmic Reticulum Stress and Disease
- Microbial Metabolic Engineering and Bioproduction
- Plant and fungal interactions
- Advanced biosensing and bioanalysis techniques
- Transgenic Plants and Applications
- Salmonella and Campylobacter epidemiology
- Microbial Fuel Cells and Bioremediation
- Neonatal and Maternal Infections
- Aquaculture disease management and microbiota
- Enterobacteriaceae and Cronobacter Research
Mahidol University
2022-2025
Siriraj Hospital
2022-2025
Kasetsart University
2012-2023
National Science and Technology Development Agency
2021-2022
National Center for Genetic Engineering and Biotechnology
2021-2022
University of Strathclyde
2017
1-Aminocyclopropane-1-carboxylate (ACC) deaminase is a plant growth promoting (PGP) trait found in beneficial bacteria including streptomycetes and responsible for stress modulation. The ACC gene, acdS, of S. venezuelae ATCC 10712 was cloned into an expression plasmid, pIJ86, to generate venezuelae/pIJ86-acdS. Expression acdS production venezuelae/pIJ86-acdS were significantly higher than the unmodified strain. deaminase-overexpressing mutant wild type control inoculated Thai jasmine rice...
Plant growth-promoting endophytic (PGPE) actinomycetes have been known to enhance plant growth and mitigate from abiotic stresses via their PGP-traits. In this study, PGPE Streptomyces sp. GKU 895 promoted alleviated salt tolerance of salt-susceptible rice cultivar IR29 by augmentation weight declined ROS after irrigation with 150 mM NaCl in a pot experiment. Transcriptome analysis exposed the combination strain salinity demonstrated up downregulated differentially expressed genes (DEGs)...
Environmental variations have been observed to influence bacterial community composition, thereby impacting biological activities in the soil. Together, information on functional groups Phatthalung sago palm-growing soils remains limited. In this work, core soil areas during both summer and rainy seasons was examined using V3-V4 amplicon sequencing. Our findings demonstrated that had no significant effects alpha diversity, but beta diversity of influenced by seasonal variations. The bacteria...
Intraamniotic infection (defined as intraamniotic inflammation with microorganisms) is an important cause of the preterm labor syndrome. Methods for detection microorganisms in amniotic fluid are culture and/or polymerase chain reaction. However, both methods take time, and results rarely available clinical decision-making. Nanopore sequencing technology offers real-time, long-read that can produce rapid results. To determine 1) diagnostic performance 16S rDNA nanopore method identification...
Actinobacteria is an ancient phylum of Gram-positive bacteria with a characteristic high GC content to their DNA. The ActinoBase Wiki focused on the filamentous actinobacteria, such as Streptomyces species, and techniques growth conditions used study them. These organisms are studied because complex developmental life cycles diverse specialised metabolism which produces many antibiotics currently in clinic. community effort that provides valuable freely accessible resources, including...
Streptomyces sp. GKU 895 is an endophytic actinomycete isolated from the roots of sugarcane. has a genome 8.3 Mbp and exhibits adaptations related to plant growth-promoting activity. It also extensive specialized metabolite biosynthetic gene clusters apparent in its genome.
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ABSTRACT The endophytic plant growth-promoting Microbispora sp. strain GKU 823 was isolated from the roots of sugarcane cultivated in Thailand. It has an estimated 9.4-Mbp genome and a G+C content 71.3%. sequence reveals several genes associated with traits extensive specialized metabolite biosynthesis.
Abstract The market for the application of probiotics as a livestock health improvement supplement has increased in recent years. However, most available products are quality-controlled using low-resolution techniques and un-curated databases, resulting misidentification incorrect product labels. In this work, we deployed two workflows compared results obtained by full-length 16S rRNA genes (16S) metagenomic (Meta) data to investigate their reliability microbial composition both liquid solid...
Swine feed-additive probiotics products play a major role in swine performance and welfare by promoting gut health. Here, we present two types of data, including full-length 16S rRNA amplicon sequence data long-read metagenomic obtained from the same commercial probiotic product.
Abstract The thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC656 is a potential host for heterologous protein production. However, overproduction of can induce cellular stress and limit the level its secretion. To improve secretion protein, we identified candidate proteins with altered production during in O. by using label‐free comparative proteomic approach. Four hundred sixty‐four various biological functions showed differential abundance between expressing fungal...
The thermotolerant methylotrophic yeast Ogataea thermomethanolica TBRC 656 is a potential host strain for industrial protein production. Heterologous proteins are often retained intracellularly in resulting endoplasmic reticulum (ER) stress and poor secretion, despite efforts to engineer secretory pathways, heterologous production lower than expected. We hypothesized that activation of genes involved the pathway could mitigate ER stress. In this study, we created mutants defective...
<title>Abstract</title> The advent of Oxford Nanopore Technologies has undergone significant improvements in terms sequencing costs, accuracy, and read lengths, making it a cost-effective, readily accessible approach for analyzing microbial genomes. A major challenge bacterial whole genome by technology is the requirement higher quality quantity high molecular weight DNA compared to short-read platforms. In this study, using eight pathogenic bacteria, we evaluated quality, quantity,...
ABSTRACT Despite their immense economic value as a key aquaculture species, the production of Pacific white shrimp ( Litopenaeus vannamei ) faces significant challenges from intensive farming practices and disease outbreaks. Routine microbial profiling for surveillance could be promising approach to anticipate control To achieve this, accuracy in ponds is crucial enabling targeted action prevention. Extensive documentation emphasizes that, beyond biological factors (related host, diet, or...
The advent of Oxford Nanopore Technologies has undergone significant improvements in terms sequencing costs, accuracy, and read lengths, making it a cost-effective, readily accessible approach for analyzing microbial genomes. A major challenge bacterial whole genome by technology is the requirement higher quality quantity high molecular weight DNA compared to short-read platforms. In this study, using eight pathogenic bacteria, we evaluated quality, quantity, fragmented size distribution...
CRISPR multiplex gRNA systems have been employed in genome engineering various industrially relevant yeast species. The thermotolerant methylotrophic Ogataea thermomethanolica TBRC 656 is an alternative host for heterologous protein production. However, the limited secretory capability of this a bottleneck Here, we refined CRISPR-based tools simultaneous mutagenesis and activation multiple pathway genes to improve secretion. We demonstrated that multiplexed CRISPR-Cas9 mutation up four (...
Analysis of feed supplements can highlight microbial diversity and the prevalence antimicrobial resistance (AMR), allowing users to monitor safety their animals. The 16S amplicon metagenomic data generated by nanopore sequencing revealed that Bacillus was dominant prokaryote, AMR genes were detected in animal probiotic products.
We report the circularized complete genome sequences, containing a circular chromosome and two plasmids, of strains SalSpp05 (4.9 Mbp) SalSpp10 (4.8 Mbp), which were isolated from chicken carcass rinse water samples; sequences obtained by combining Oxford Nanopore Technologies long-read data Illumina short-read data. Whole-genome alignments indicated that both belong to Salmonella enterica.