A5101619968- Cell death mechanisms and regulation
- Cancer-related Molecular Pathways
- Inflammatory Bowel Disease
- RNA Interference and Gene Delivery
- Endoplasmic Reticulum Stress and Disease
- Cancer Research and Treatments
- PARP inhibition in cancer therapy
- Myasthenia Gravis and Thymoma
- Genomics and Phylogenetic Studies
- Lung Cancer Treatments and Mutations
- Microbial Community Ecology and Physiology
- Epigenetics and DNA Methylation
- Autoimmune Neurological Disorders and Treatments
- IL-33, ST2, and ILC Pathways
- Advanced biosensing and bioanalysis techniques
- Peripheral Neuropathies and Disorders
- Autophagy in Disease and Therapy
- Balance, Gait, and Falls Prevention
- Protein Degradation and Inhibitors
- Curcumin's Biomedical Applications
- Acute Ischemic Stroke Management
- Immune cells in cancer
- Histone Deacetylase Inhibitors Research
- Skin Protection and Aging
- Amyloidosis: Diagnosis, Treatment, Outcomes
Asan Medical Center
2014-2024
Ulsan College
2015-2024
Michigan State University
2024
University of Ulsan
2015-2024
Grand Rapids Community College
2024
Korea University
2019-2024
Trinity Health
2024
Samsung Medical Center
2023
Sungkyunkwan University
2023
Seoul Medical Center
2021
CRISPR-based base editors (BEs) are widely used to induce nucleotide substitutions in living cells and organisms without causing the damaging DNA double-strand breaks donor templates. Cytosine BEs that C:G T:A conversion adenine A:T G:C have been developed. Various attempts made increase efficiency of both BEs; however, their activities need be improved for further applications. Here, we describe a fluorescent reporter-based drug screening platform identify novel chemicals with goal...
The heat shock protein ClpB in Escherichia coli is a protein-activated ATPase and consists of two proteins with sizes 93 79 kDa. By polymerase chain reaction-aided site-directed mutagenesis, both the have been shown to be encoded by same reading frame clpB gene, 93-kDa (ClpB93) from 5'-end AUG translational initiation site 79-kDa (ClpB79) 149th codon (an internal GUG start site). Both purified ClpB93 ClpB79 behave as tetrameric complexes very similar size about 350 kDa upon gel filtration on...
A Gram-negative-staining, non-spore-forming bacterium devoid of flagella, designated strain B9T, was isolated from rice paddy soil associated with the roots Oryza sativa collected Jinju, South Korea. Cells were straight rods, catalase- and oxidase-positive able to hydrolyse pectin, xylan laminarin. Growth B9T observed between 15 35 °C (optimum 25–30 °C) pH 5.0 8.0 6.5–7.5). Strain contained menaquinone-7 (MK-7) as a major isoprenoid quinone summed feature 3 (C16 : 1 ω7c and/or iso-C15 0...
Breast cancer cells generally develop resistance to TNF-Related Apoptosis-Inducing Ligand (TRAIL) and, therefore, assistance from sensitizers is required. In our study, we have demonstrated that Spleen tyrosine kinase (Syk) inhibitor Bay 61–3606 was identified as a TRAIL sensitizer. Amplification of TRAIL-induced apoptosis by accompanied the strong activation Bak, caspases, and DNA fragmentation. mechanism action, sensitized via two mechanisms regulating myeloid cell leukemia sequence-1...
Pancreatic cancer is one of the most difficult cancers to cure due lack early diagnostic tools and effective therapeutic agents. In this study, we aimed isolate new bioactive compounds that effectively kill pancreatic ductal adenocarcinoma (PDAC) cells, but not untransformed, human epithelial (HPDE) cells. To end, established four primary PDAC cell lines screened 4141 from bioactive-compound libraries. Initial screening yielded 113 hit caused over a 50% viability reduction in all tested...
An aerobic, pale-orange-pigmented, Gram-stain-negative bacterium, designated strain EM39T, was isolated from seawater the eastern coast of Jeju Island, Korea, and its taxonomic status established using a polyphasic approach. Comparative 16S rRNA gene sequence studies revealed that EM39T formed distinct lineage within family Flavobacteriaceae could be distinguished strains members related genera Gaetbulibacter, Mariniflexile Tamlana by analysis (similarity values between were all less than...
Tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL) has been characterized as an anti‐cancer therapeutic agent with prominent cancer cell selectivity over normal cells. However, breast cells are generally resistant to TRAIL, thus limiting its potential. In this study, we found that BIX‐01294, a selective inhibitor of euchromatin histone methyltransferase 2/G9a, is strong TRAIL sensitizer in The combination BIX‐01294 and decreased viability led increase the annexin V/propidium...
Lambert-Eaton myasthenic syndrome (LEMS) is an autoimmune neuromuscular disorder caused by pathogenic autoantibodies directed against voltage-gated calcium channels present on the presynaptic nerve terminal. For LEMS patients refractory to initial symptomatic treatment with amifampridine, immunomodulatory therapy intravenous immunoglobulin (IVIG) often utilized. However, in authors' review of literature, utility subcutaneous (SCIG) has been scarcely reported. Here, we a unique case...
Gemcitabine is a nucleoside analog widely used as an anticancer agent against several types of cancer. Although gemcitabine sometimes shows excellent effectiveness, cancer cells are often poorly responsive to or resistant the drug. Recently, specific strains dysbiosis human microbiome were correlated with drug reactivity and resistance acquisition. Therefore, we aimed identify antibiotic compounds that can modulate enhance responsiveness gemcitabine. To achieve this, confirmed based on...
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Creeping fat [CF] is a poorly understood feature of Crohn's disease [CD], characterized by the wrapping mesenteric adipose tissue [MAT] around inflamed intestine. The aim this study was to investigate transcriptional profile and compositional features CF. We collected 59 MAT samples: 23 paired samples from patients with CD (CF [CD-CF] uninflamed intestine [CD-MAT]) 13 non-CD [Con-MAT]. Differentially expressed gene [DEG], functional pathway, cell deconvolution, co-expression network analyses...
The importance of the P1 reactive site for specificity ecotin on target proteases was examined by site-directed mutagenesis. replacement Met at with Ile, Arg, Glu, or Tyr showed little no effect ability to inhibit trypsin. Similar results were obtained chymotrypsin, except that its Glu caused about 40% reduction inhibitory activity ecotin. On other hand, residue Tyr, dramatically reduced elastase, while Ile effect. Nevertheless, elastase could be completely inhibited upon incubation excess...
Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a potential biological anticancer agent. However, wide range of human primary cancers, including pancreatic cancer, display resistance to apoptosis induction by TRAIL. Therefore, this needs be overcome allow TRAIL successfully used in cancer therapy. In study, we performed compound screen isolate sensitizers and found that one the identified compounds, 7-benzylidenenaltrexone maleate (BNTX), sensitized cells TRAIL-induced...