A5070525221- Retinoids in leukemia and cellular processes
- Male Breast Health Studies
- Cancer Treatment and Pharmacology
- Cancer-related Molecular Pathways
- Sirtuins and Resveratrol in Medicine
- Ubiquitin and proteasome pathways
- Histone Deacetylase Inhibitors Research
- DNA Repair Mechanisms
- Nuclear Structure and Function
- PARP inhibition in cancer therapy
- Spaceflight effects on biology
- Cancer-related cognitive impairment studies
- Cardiovascular and Diving-Related Complications
- Carcinogens and Genotoxicity Assessment
Institute of Cytology
2020-2024
Peter the Great St. Petersburg Polytechnic University
2020
Natural aging and age-related diseases involve the acceleration of replicative aging, or senescence. Multiple proteins are known to participate in these processes, including promyelocytic leukemia (PML) protein, which serves as a core component nuclear-membrane-less organelles PML nuclear bodies (PML-NBs). In this work, morphological changes PML-NBs alterations protein localization at transition primary fibroblasts senescent state were studied by immunofluorescence. The obtained from both...
Different programs are used for processing and analyzing of fluorescent images.However, some these produce uncertain results due to their dependence on human factor.Here we made a comparative study three images analysis: IPLab, Fiji DARFI.The were compared by the fluorescence intensity measurement quality cell nucleus area detection.We also analyzed counting foci in nucleus, determination intensity.Fluorescent obtained with laser microscope Zeiss Axiovert 200M after indirect...
Cell mosaicism is found in biological systems much more often than clinically identified forms of the disease, some cases, "erased forms” or "normal variants” are phenotypic manifestations mosaicism. Some diseases, difficult for a clinical diagnosis, such as ataxia-telangiectasia, based on cell This work aimed to study DNA repair disorders lines dermal fibroblasts isolated from skin biopsies 5 patients with diagnosed ataxia-telangiectasia. In obtained lines, method indirect...
In the cell lines of dermal fibroblasts 30 and 55 year-old patients with breast cancer (BC) in three medical history cancer, features DNA repair were studied.The amount, intensity fluorescence area foci protein 53BP1 histone γH2AX investigated at different time parameters field irradiation cells a dose 2 Gy.A line from 30-year-old patient no was used as healthy donor.The results showed that reparative curve donor differed curves remaining lines.The young had more serious violations...