A5068255075- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Click Chemistry and Applications
- Cellular transport and secretion
- Receptor Mechanisms and Signaling
- Image Processing Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Adenosine and Purinergic Signaling
- Advanced Electron Microscopy Techniques and Applications
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Luminescence and Fluorescent Materials
- Nanoplatforms for cancer theranostics
- Retinal Development and Disorders
- bioluminescence and chemiluminescence research
- Biotin and Related Studies
- Hepatitis B Virus Studies
- Advanced MRI Techniques and Applications
- Cell Image Analysis Techniques
- Nitric Oxide and Endothelin Effects
- Anesthesia and Neurotoxicity Research
- Electrochemical sensors and biosensors
Graco (United States)
2019-2023
The University of Tokyo
2012-2023
Tokyo Medical University
2009-2019
The Open University of Japan
2014
Nagoya University
2007-2009
University of Tsukuba
1997-2000
Tokyo University of Science
1970
Glutamate is the major neurotransmitter in brain, mediating point-to-point transmission across synaptic cleft excitatory synapses. Using a glutamate imaging method with fluorescent indicators, we show that activity generates extrasynaptic dynamics vicinity of active These had magnitudes and durations sufficient to activate receptors brain slices. We also observed crosstalk between synapses--i.e., summation released from neighboring Furthermore, successfully sensory input extremities induced...
A fluorescent aptamer sensor was applied to the analysis of extracellular chemical transmitter dynamics. We utilized a tocopherol-labeled aptamer, which allowed direct anchoring on cell surface while retaining its performance as sensor. The fast-responsive DNA sensor, targets adenine compounds, anchored brain astrocytes. Fluorescence imaging aptamer-anchored astrocytes enabled real-time monitoring release compounds gliotransmitter, synchronized with calcium wave propagation in neighboring cells.
Abstract Live imaging of exocytosis dynamics is crucial for a precise spatiotemporal understanding secretion phenomena, but current approaches have serious limitations. We designed and synthesized small‐molecular fluorescent probes that were chemically optimized sensing acidic intravesicular pH values, established they can be used to sensitively reliably visualize vesicular following stimulation. This straightforward technique the visualization as well endocytosis/reacidification processes...
ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTHigh-Performance Caged Nitric Oxide: A New Molecular Design, Synthesis, and Photochemical ReactionShigeyuki Namiki, Tatsuo Arai, Ken FujimoriView Author Information Department of Chemistry, University Tsukuba Tsukuba, 305 JapanCite this: J. Am. Chem. Soc. 1997, 119, 16, 3840–3841Publication Date (Web):April 23, 1997Publication History Received13 August 1996Published online23 April 1997Published inissue 1...
Abstract Imaging neurotransmission is expected to greatly improve our understanding of the mechanisms and regulations synaptic transmission. Aiming at imaging glutamate, a major excitatory neurotransmitter in CNS, we developed novel optical glutamate probe, which consists ligand‐binding domain α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA) receptor GluR2 subunit small molecule fluorescent dye. We that such conjugates might report microenvironmental changes upon protein...
Adenosine 5’ triphosphate (ATP) is a ubiquitous extracellular signaling messenger. Here, we describe method for in-vivo imaging of ATP with high spatiotemporal resolution. We prepared comprehensive set cysteine-substitution mutants ATP-binding protein, Bacillus FoF 1 -ATP synthase ε subunit, labeled small-molecule fluorophores at the introduced cysteine residue. Screening revealed that Cy3-labeled glutamine-105 mutant (Q105C-Cy3; designated ATPOS) shows large fluorescence change in presence...
Nitric oxide (NO) is an intercellular messenger regulating neuronal functions. To visualize NO signalling in the brain, we generated a novel fluorescent indicator, which consists of heme‐binding region (HBR) soluble guanylyl cyclase and green protein. The indicator (HBR–GFP) was expressed Purkinje cells mouse cerebellum imaged signals acute cerebellar slices upon parallel fibre (PF) activation with train burst stimulations (BS, each BS consisting five pulses at 50 Hz). Our results showed...
Abstract Fluorescent sensors are powerful tools for visualizing cellular molecular dynamics. We present a high‐throughput screening system, designated hybrid‐type fluorescence indicator development (HyFInD), to identify optimal position‐specific fluorophore labeling in consisting of combinations ligand‐binding protein mutants with small fluorophores. screened glutamate among hybrid molecules obtained by the reaction four cysteine‐reactive probes set cysteine‐scanning 274 amino acid S1S2...
Abstract The terrestrial slug Limax has the ability to learn odor associations. This depends on function of procerebrum, secondary olfactory center in brain. Among various neurotransmitters that are thought be involved glutamate is one most important molecules. However, existence and this system have been proposed solely basis a few lines indirect evidence from pharmacological experiments. In present study, we demonstrated release as neurotransmitter procerebrum , by using three different...
Abstract Neurotransmitters are released by exocytosis from synaptic vesicles at the active zone in presynaptic terminal. The scaffold of consists only a few evolutionarily conserved proteins, including RIM, CAST/ELKS, and RIM-BP, tethers Munc13 Ca 2+ channels. molecular principles that enable these proteins to mediate diversity have remained unclear. Here, we identified synapse type-specific nanoconfigurations systematic quantification using nanoscopy two types excitatory synapses inhibitory...
Abstract Live imaging of exocytosis dynamics is crucial for a precise spatiotemporal understanding secretion phenomena, but current approaches have serious limitations. We designed and synthesized small‐molecular fluorescent probes that were chemically optimized sensing acidic intravesicular pH values, established they can be used to sensitively reliably visualize vesicular following stimulation. This straightforward technique the visualization as well endocytosis/reacidification processes...
Disc1 is a susceptibility gene for psychiatric disorders including schizophrenia. It has been suggested that excess transmission through dopamine type 2 receptors (D2Rs) in the striatum an underlying mechanism of pathogenesis. In this study, we used super-resolution microscopy to study distribution D2Rs at nanoscale mice lacking exons and 3 (Disc1-deficient mice). We found nucleus accumbens (NAc) wild-type form nanoclusters (~ 20,000 nm2), Disc1-deficient have larger more D2R than mice....
Abstract Microglia are resident immune cells in the central nervous system, showing a regular distribution. Advancing microscopy and image processing techniques have contributed to elucidating microglia’s morphology, dynamics, However, mechanism underlying distribution of microglia remains be elucidated. First, we quantitatively confirmed regularity pattern microglial soma retina. Second, formulated mathematical model that includes factors may influence Next, experimentally quantified...
The alpha,alpha-gem-difluorination of 2',4'-difluoro-alpha-(methylthio)acetophenone (1a) with N-fluoropyridinium salts gave 2',4',alpha,alpha-tetrafluoro-alpha-(methylthio)acetophenone (3a). This reaction was accelerated by the addition zinc chloride, bromide or anhydrous iron(III) and higher yields than without additives were obtained. gem-difluorination using FP-T300 in presence applicable to other alpha-(alkylthio)acetophenone derivatives (1).
A new chemotherapeutic agent, AB206, shows potent antibacterial activity against gram-negative bacteria, including most of the nalidixic acid-resistant strains tested. It strongly inhibits deoxyribonucleic acid (DNA) synthesis in Escherichia coli, but only slightly ribonucleic and protein synthesis. Its on DNA vivo vitro is greater than that acid. AB206 also acid-susceptible -resistant clinical isolates Proteus Serratia. high penetrability into E. coli cells, as demonstrated by with or...
The excited triplet state of N,N′-dimethyl-N,N′-dinitroso-p-phenylenediamine (BNN3, λmax: 299 nm), caged nitric oxide (ET: 230 kJ mol−1), was found to readily undergo N–N bond homolysis release NO. This suggests that by covalently binding a sensitizing chromophore, e.g., biacetyl, BNN3 N-methyl group, its NO photoreleasing light can be switched from UV visible, less cytotoxic light, such as 425 nm.
Adenosine 5'-triphosphate (ATP) works as an extracellular signaling molecule for cells in the brain, such neurons and glia. Cellular communication via release of ATP is involved a range processes required normal brain functions, aberrant associated with disorders. To investigate mechanisms underlying these cellular processes, various techniques have been developed measurement ATP. monitor dynamics high spatiotemporal resolution, we recently hybrid-type optical sensor (ATPOS) that enables