Abstract Methods using genomic information to forecast potential population maladaptation climate change or new environments are becoming increasingly common, yet the lack of model validation poses serious hurdles toward their incorporation into management and policy. Here, we compare estimates derived from two methods—Gradient Forests (GF offset ) risk non‐adaptedness (RONA)—using exome capture pool‐seq data 35 39 populations across three conifer taxa: Douglas‐fir varieties jack pine. We...

Several recent studies have detected and described complexes of cryptic sibling species in the genus Merodon (Diptera, Syrphidae). One representative these is avidus complex that contains four species, which proven difficult to distinguish using traditional morphological characters. In present study, we use two geometric morphometric approaches, as well molecular characters 5’-end mtDNA COI gene, delimit taxa. Analyses based on data were used strengthen boundaries within complex, validate...

Summary Understanding the genetic basis of how plants defend against pathogens is important to monitor and maintain resilient tree populations. Swiss needle cast (SNC) Rhabdocline (RNC) epidemics are responsible for major damage forest ecosystems in North America. Here we investigate architecture tolerance resistance diseases Douglas‐fir ( Pseudotsuga menziesii ) caused by two fungal pathogens: SNC Nothophaeocryptopus gaeumannii , RNC pseudotsugae . We performed case–control genome‐wide...

In recent decades, Dothistroma needle blight (DNB), a pine tree disease caused by the fungal pathogen septosporum, has severely damaged lodgepole (Pinus contorta Dougl. ex. Loud.) in British Columbia, Canada, and raised health concerns for jack banksiana Lamb.). The already shown signs of host shift eastward to hybrid populations between × P. banksiana), possibly into pure pine. However, we have little knowledge about mechanisms resistance D. especially underlying genetic basis variation...

The Merodon aureus species group (Diptera: Syrphidae: Eristalinae) comprises a number of different sub-groups and complexes. In this study we focus on resolving the taxonomic status entity previously identified as M. cinereus B, here atratus complex. We used an integrative approach based morphological descriptions, combined with supporting characters that were obtained from molecular analyses mitochondrial cytochrome c oxidase I gene well geometric morphometry wing surstylus shapes...

Eumerus is one of the most diverse genera hoverfly worldwide. Species delimitation within genus considered to be difficult due to: (a) lack an efficient key; (b) non-defined taxonomical status a large number species; and (c) blurred nomenclature. Here, we present first molecular study delimit species by using fragment mitochondrial cytochrome-c oxidase subunit I gene (COI) gene. We assessed 75 specimens assigned 28 taxa originating from two biogeographic zones: 22 western Palaearctic six...

Abstract Methods using genomic information to forecast potential population maladaptation climate change are becoming increasingly common, yet the lack of model validation poses serious hurdles toward their incorporation into management and policy. Here, we compare estimates derived from two methods – Gradient Forests (GF offset ) Risk Of Non-Adaptedness (RONA) exome capture pool-seq data 35 39 populations across three conifer taxa: Douglas-fir varieties jack pine. We evaluate sensitivity...

The Merodon aureus group is characterized by high endemism and the presence of morphologically cryptic species. Within one its subgroups, M. bessarabicus, seven species four more complexes have been described to date. One these complexes, luteomaculatus, comprises new taxa that are subject present study. Its members allopatric ranges restricted Balkan Peninsula Aegean islands. This complex exhibits morphological variability could not be using a traditional approach. Thus, we used integrative...

An ongoing study of the genus Merodon Meigen, 1803 in Republic South Africa (RSA) has revealed existence new species related to M. melanocerus Bezzi, 1915. The subgroup belongs Afrotropical lineage desuturinus group. Revision all available material from museums and detailed analyses newly -collected specimens our own expeditions RSA resulted delimitation five species: capensis Hurkmans sp. n., commutabilis Radenković et Vujić drakonis flavocerus n. melanocerus. In addition classical...

Many conifers have distributions that span wide ranges in both biotic and abiotic conditions, but the basis of response to stress has received much less attention than stress. In this study, we investigated gene expression lodgepole pine (Pinus contorta) attack by fungal pathogen Dothistroma septosporum, which causes needle blight, a disease caused severe climate-related outbreaks northwestern British Columbia. We inoculated tolerant susceptible pines with two D. septosporum isolates...

Abstract Examination of 122 specimens Xanthogramma Schiner, 1861 (Diptera: Syrphidae) from varied localities in Europe (+Turkey) resulted the description two new species ( X. aeginae Ricarte, Nedeljković, and Vujić pilosum ), as well data on six other species. Most examined material originated Balkan Peninsula Greek islands. New concepts were supported by morphological molecular evidence. Relationships among eight studied analysed discussed based nuclear (ITS2) mitochondrial (COI) genes...

Abstract Despite their suitability for studying evolution, many conifer species have large and repetitive giga‐genomes (16–31 Gbp) that create hurdles to producing high coverage SNP data sets capture diversity from across the entirety of genome. Due in part multiple ancient whole genome duplication events, gene family expansion subsequent evolution within Pinaceae , false misalignment paralog copies creates further challenges accurately reproducibly inferring evolutionary history sequence...

Brief introduction: The European brown hare ( Lepus europaeus Pallas, 1778) is the only species that can be found in Serbia, and Balkan Peninsula where it represents an important game species. Previous analyses of hares Serbia based on allozyme mtDNA sequence variability revealed moderate genetic diversity indicated possible population structuring region. aim this study to detect structure from using microsatellites. Material Methods: A total number 157 were collected six different regions...

In recent decades, Dothistroma needle blight (DNB), a pine tree disease caused by the fungal pathogen septosporum , has severely damaged lodgepole ( Pinus contorta Dougl. ex. Loud.) in British Columbia, Canada, and raised health concerns for jack banksiana Lamb.). The already shown signs of host shift eastward to hybrid populations between × P. ), possibly into pure pine. However, we have little knowledge about mechanisms resistance D. especially underlying genetic basis variation pines....

Understanding the genetic architecture of tolerance and resistance to pathogens is important monitor maintain resilient tree populations. Here we investigate basis needle cast disease in Douglas-fir (Pseudotsuga menziesii) caused by two fungal pathogens: Swiss (SNC) Nothophaeocryptopus gaeumannii, Rhabdocline (RNC) pseudotsugae). We performed a case-control genome-wide association analysis (GWAS) found these traits be polygenic. Significant associations with SNC were for SNPs genes stomatal...

Microsatellite (SSR) markers with known precise intrachromosomal locations are widely used for mapping genes in rye and the investigation of wheat-rye translocation lines triticale highly demanded economically important QTL-analysis. One sources novel SSR microsatellites transferable from wheat genome. Broadening list available SSRs mapped to chromosomes is still needed, since some chromosome maps have just a few microsatellite loci mapped. The goal current study was integrate EST-SSRs into...

Abstract Despite their suitability for studying evolution, many conifer species have large and repetitive giga-genomes (16-31Gbp) that create hurdles to producing high coverage SNP datasets captures diversity from across the entirety of genome. Due in part multiple ancient whole genome duplication events, gene family expansion subsequent evolution within Pinaceae , false misalignment paralog copies further challenges accurately reproducibly inferring evolutionary history sequence data. Here,...