A5023260054- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Pluripotent Stem Cells Research
- Cytomegalovirus and herpesvirus research
- Pancreatic function and diabetes
- Adenosine and Purinergic Signaling
Stanford University
2018-2024
The University of Texas Health Science Center at San Antonio
2017
The University of Texas Health Science Center at Houston
2017
Abstract The intensive nutrient requirements needed to sustain T cell activation and proliferation, combined with competition for nutrients within the tumor microenvironment, raise prospect that glucose availability may limit CAR-T function. Here, we seek test hypothesis stable overexpression (OE) of transporter GLUT1 in primary human cells would improve their function antitumor potency. We observe GLUT1OE increases consumption, glycolysis, glycolytic reserve, oxidative phosphorylation,...
Antibody class-switch DNA recombination (CSR) is initiated by AID-introduced DSBs in the switch (S) regions targeted for recombination, as effected Ku70/Ku86-mediated NHEJ. Ku-deficient B cells, however, undergo (reduced) CSR through an alternative(A)-NHEJ pathway, which introduces microhomologies S-S junctions. As microhomology-mediated end-joining requires annealing of single-strand ends, we addressed contribution factors HR Rad52 and translesion polymerase θ to CSR. Compared with their...
Stem cell–based approaches have the potential to address organ shortage in transplantation. Whereas both embryonic stem cells and induced pluripotent been utilized as cellular sources for differentiation lineage specification, their relative ability be recognized by immune effector is unclear. We determined expression of recognition molecules on hepatocyte-like (HLC) generated from murine cells, compared adult hepatocytes, we evaluated impact natural killer (NK) cells. report that HLC lack...
Abstract Chimeric antigen receptors (CARs) are synthetic that link an extracellular tumor specific domain to intracellular signaling domains. Despite of remarkable results against refractory B cell malignancies, intense effort is underway augment the potency CAR T cells in order overcome suppressive microenvironment, which associated with exhaustion. Adenosine a major mediator immune suppression. Ectoenzyme CD39 plays central role generation adenosine by catalyzing metabolism ATP into...