A5111672617- Urinary Bladder and Prostate Research
- Pelvic floor disorders treatments
- Soil and Unsaturated Flow
- Sexual function and dysfunction studies
- Urinary Tract Infections Management
- Cellular transport and secretion
- Soil Management and Crop Yield
- Endoplasmic Reticulum Stress and Disease
- Plant responses to water stress
- Fungal and yeast genetics research
- Rice Cultivation and Yield Improvement
- Ecology and Vegetation Dynamics Studies
- Soil Mechanics and Vehicle Dynamics
- Tree Root and Stability Studies
- Nematode management and characterization studies
- Soil Carbon and Nitrogen Dynamics
- Geotechnical Engineering and Soil Stabilization
- Plant Water Relations and Carbon Dynamics
- Hormonal and reproductive studies
- Prostate Cancer Diagnosis and Treatment
- Musculoskeletal pain and rehabilitation
- Soil Moisture and Remote Sensing
- Hydrology and Watershed Management Studies
- Soybean genetics and cultivation
- Fibromyalgia and Chronic Fatigue Syndrome Research
National Institute of Diabetes and Digestive and Kidney Diseases
2014-2024
National Institutes of Health
2014-2024
Medical University of South Carolina
2017
Kansas State University
2017
Office of the Director
2016
University of Chicago
2015
Northwestern University
2014-2015
NorthShore University HealthSystem
2015
University of Pennsylvania
2015
Temple University
2015
Lysosomes are membrane-bound cytoplasmic organelles involved in intracellular protein degradation. They contain an assortment of soluble acid-dependent hydrolases and a set highly glycosylated integral membrane proteins. Most the properties lysosomes shared with group cell type-specific compartments referred to as 'lysosome-related organelles', which include melanosomes, lytic granules, MHC class II compartments, platelet-dense basophil azurophil Drosophila pigment granules. In addition...
Formation of intracellular transport intermediates and selection cargo molecules are mediated by protein coats associated with the cytosolic face membranes. Here, we describe a novel family ubiquitous coat proteins termed GGAs, which includes three members in humans two yeast. GGAs have modular structure consisting VHS domain, region homology GAT, linker segment, to ear domain γ-adaptins. Immunofluorescence microscopy showed colocalization Golgi markers, whereas immunoelectron GGA3 revealed...
Germination provides many potentially unrecognized sources of variation in the regeneration niche. In this study we relate germination requirements and seed size for 16 species pioneer trees to microclimatic conditions present gaps semi-deciduous rain forest Panama. We found that, whereas increased duration direct irradiance can be an effective indicator presence a canopy gap across all scales openness, diel fluctuations soil temperature effectively discriminate both understory sites small...
Here we report the identification and characterization of AP-4, a novel protein complex related to heterotetrameric AP-1, AP-2, AP-3 adaptors that mediate sorting in endocytic late secretory pathways. The key this was cloning sequencing two widely expressed, mammalian cDNAs encoding new homologs adaptor β ς subunits named β4 ς4, respectively. An antibody recognized human cells an ∼83-kDa polypeptide exists both soluble membrane-associated forms. Gel filtration, sedimentation velocity,...
The “Multidisciplinary Approach to the Study of Chronic Pelvic Pain” (MAPP) Research Network was established by NIDDK better understand pathophysiology urologic chronic pelvic pain syndromes (UCPPS), inform future clinical trials and improve care. evolution, organization, scientific scope MAPP Network, unique approach network’s central study common data elements are described. primary protocol for Trans-MAPP Epidemiology/Phenotyping (EP) comprises a multi-site, longitudinal observational...
Abstract Chronic pain is often measured with a severity score that overlooks its spatial distribution across the body. This widespread believed to be marker of centralization, central nervous system process decouples perception from nociceptive input. Here, we investigated whether centralization manifested at level brain using data 1079 participants in Multidisciplinary Approach Study Pelvic Pain Research Network (MAPP) study. Participants clinical diagnosis urological chronic pelvic...
The pathophysiology of interstitial cystitis/painful bladder syndrome remains incompletely understood but is thought to involve central disturbance in the processing pain and viscerosensory signals. We identified differences brain activity connectivity between female patients with healthy controls advance clinical phenotyping treatment efforts for syndrome.
No AccessJournal of UrologyAdult Urology1 Feb 2016Assessment the Lower Urinary Tract Microbiota during Symptom Flare in Women with Urologic Chronic Pelvic Pain Syndrome: A MAPP Network Study J. Curtis Nickel, Alisa Stephens, Richard Landis, Chris Mullins, Adrie van Bokhoven, M. Scott Lucia, Garth D. Ehrlich, and Research Network† NickelJ. Nickel Department Urology, Queen's University, Kingston, Ontario, Canada , StephensAlisa Stephens Biostatistics Epidemiology, Perelman School Medicine,...
Summary 1 Soil and plant-water relations were studied in a canopy opening surrounding forest shade moist tropical Ghana using seedlings of two tree species with different regeneration strategies, the pioneer Terminalia superba nonpioneer light demander Entandrophragma utile. 2 During consecutive dry seasons, soil matric potential varied from -30 kPa at end rainy season, to below -2.5 MPa middle season. part first season was higher gap than forest. In second no differences observed. 3 Leaf...
SUMMARY Pastes containing sand and 0, 5, 20 80 g kaolin kg −1 mixture were equilibrated at matric potentials of – 2, 10, 100 1000 kN m −2 or allowed to air‐dry. The strength cylindrical samples was determined in unconfined compression by the indirect tensile test. Measured strengths are explained using Coulomb‐Mohr theory concept effective stress. At pore water tensions greater than 10 stress dominant factor determining compressive strength. contrast between this behaviour that friable...
Abstract. Hard‐setting soils are widespread in dry regions. Their properties described and a physical explanation for hard‐setting behaviour is given. The limitations on soil management fertility caused by depend timing of rainfall or irrigation with respect to cultivations crop development, much research needed quantify reductions yield imposed these limitations.
We used next-generation, state-of-the-art, culture independent methodology to survey urine microbiota of males with urologic chronic pelvic pain syndrome and control participants enrolled in the MAPP Network investigate a possible microbial etiology.Male patients matched controls were asked provide initial, midstream post-prostatic massage specimens. Specimens analyzed Ibis T-5000 Universal Biosensor technology comprehensive identification bacterial select fungal species. Differences between...
Studies have suggested chronic pain syndromes are associated with neural reorganization in specific regions perception, processing, and integration of pain. Urological pelvic syndrome (UCPPS) represents a collection characterized by pain, namely Chronic Prostatitis/Chronic Pelvic Pain Syndrome (CP/CPPS) Interstitial Cystitis/Painful Bladder (IC/PBS), that both poorly understood their pathophysiology, treated ineffectively. We hypothesized patients UCPPS may microstructural differences the...
Brain network activity associated with altered motor control in individuals chronic pain is not well understood. Chronic Prostatitis/Chronic Pelvic Pain Syndrome (CP/CPPS) a debilitating condition which previous studies have revealed resting pelvic floor muscle men CP/CPPS compared to healthy controls. We hypothesized that the brain networks controlling muscles would also show state function CP/CPPS. Here we describe results of first test this hypothesis focusing on cortical regions, termed...
Summary A filter–paper technique that can be used to measure the matric potential of field soil is described in detail. When a calibrated batch filter papers used, precision limited by variability between individual and limitation weighing nearest mg. The absolute accuracy also instruments for calibration. There was reasonable agreement batches same type paper, suggesting our generalized calibration curves Whatman No. 42 where not paramount. For potentials ≥−2.5 MPa, need equilibrated with 6...
Chronic pain symptoms often change over time, even in individuals who have had for years. Studying biological factors that predict trends symptom chronic may uncover novel pathophysiological mechanisms and potential therapeutic targets. In this study, we investigated whether brain functional connectivity measures obtained from resting-state magnetic resonance imaging at baseline can longitudinal (3, 6, 12 months after scan) urologic pelvic syndrome. We studied 52 with syndrome (34 women, 18...