The tomato leaf miner, Tuta absoluta (Lepidoptera) is a significant pest of tomatoes that has undergone rapid expansion in its range during the past six years and now present across Europe, North Africa parts Asia. One main means controlling this through use chemical insecticides. In current study insecticide bioassays were used to determine susceptibility five T. strains established from field collections Europe Brazil pyrethroids. High levels resistance λ cyhalothrin tau fluvalinate...

Biological control is an efficient pest method but there are still limitations that hindering its wider adoption. Genetic improvement of biological agents (BCAs) can help to overcome these constraints, the choice key attributes for better performance need be selected open question. Several characteristics have been suggested harsh reality selective breeding BCAs has received a lot attention resulted in very little progress. Identifying appropriate traits prioritized may first step reverse...

Abstract The genetic structure of six Iberian populations the whitefly Bemisia tabaci , two them biotype Q, one B, and other three a mixture both, has been studied using random amplified polymorphic DNA–polymerase chain reaction (RAPD–PCR). A total 336 individuals was completely discriminated by means 234 scored bands. Separate analyses molecular variance haploid males diploid females pairwise number differences between haplotypes showed that biotypes contribute significantly more to...

Abstract BACKGROUND: Bemisia tabaci Gennadius Q‐biotype has readily developed resistance to numerous insecticide classes. Studies in the Mediterranean area are needed clarify status and cross‐resistance patterns this invasive whitefly biotype. The levels of nymphs seven strains B. from south‐eastern Spain representative insecticides were determined. RESULTS: Six populations had low moderate azadirachtin (0.2‐ 7‐fold), buprofezin (11‐ 59‐fold), imidacloprid (1‐ 15‐fold), methomyl (3‐...

Abstract BACKGROUND: Before its introduction into Europe at the end of 2006, Tuta absoluta (Povolny, 1994) was confined solely to South America. Currently, this invasive pest is well established in various European and Mediterranean countries, causing important economic losses tomato ( Lycopersicon esculentum Mill.) crops. In order study genetic variability pest, 23 ten native American populations were analysed with nuclear ribosomal DNA (rDNA) mitochondrial (mtDNA) markers. RESULTS: The...

The whitefly Bemisia tabaci is vector of plant infecting viruses and it considered as one the most important agricultural pests around Mediterranean basin. At present, five biotypes B. have been reported in Basin: B, Q, S, T M. To establish phylogeographic relationship these with others, 54 samples collected Europe Africa were analysed by sequencing mitochondrial cytochrome oxidase I gene (mtCOI). phylogeny showed that Spanish corresponding to biotype S related haplotype Uganda 2 African...

Abstract BACKGROUND Insecticide resistance in the natural enemies of pest species is a very desirable trait. It allows better integration biological control with synthetic and compounds applied to manage certain pests, especially secondary outbreaks. predatory insects has been documented for lady beetles lacewings, but intriguingly no cases field‐evolved have noted any heteropteran predator. In this work, we first explored variation susceptibility pyrethroids wild commercial populations...

Abstract BACKGROUND: The western flower thrips (WFT), Frankliniella occidentalis (Pergande), is an economically important pest. genetic basis of acrinathrin resistance was investigated in WFT. RESULTS: resistant strain, selected the laboratory for from a pool populations collected Almeria (south‐eastern Spain), showed high to (43‐fold based on LC 50 values) compared with susceptible strain. Mortality data reciprocal crosses and indicated that autosomal not influenced by maternal effects....

Control of Frankliniella occidentalis (Pergande) is a serious problem for agriculture all over the world because limited range insecticides that are available. Insecticide resistance in F. has been reported major insecticide groups. Our previous studies showed cytochrome P450-mediated detoxification mechanism responsible this pest. Degenerate polymerase chain reaction was used to identify P450 genes might be involved acrinathrin resistance, laboratory population occidentalis. Associated...

Abstract Nine isolates of Alternaria causing black rot 'Fortune' mandarin were obtained from a commercial orchard in the Murcia region south-east Spain. The isolates, according to their morphological and physiological characteristics, identified as A. alternata. Analysis DNA sequences internal transcribed spacers 1 (ITS1) 2 (ITS2) ribosomal corroborated identification. In this work, we evaluated pathogenicity each nine on fruits leaves well fruit susceptibility pathogen at different growth...

Abstract The African citrus psyllid Trioza erytreae is one of the major threats to industry as vector incurable disease known huanglongbing (HLB) or greening. invaded northwest Iberian Peninsula 6 years ago. invasion alarmed growers in Mediterranean basin, largest producing area Europe, which still free HLB. Before our study, no research had been carried out on genetic diversity T. populations that have and archipelagos Macaronesia (Madeira Canary Islands). In this combining microsatellites...

Abstract Augmentative biological control in protected crops relies mainly on omnivorous predators. Their performance as agents (BCA) depends several characteristics of the species, which turn may differ among strains within a species. We have recently reported achievement two Orius laevigatus (Hemiptera: Anthocoridae) showing significant larger body size or better fitness when feeding pollen, having key impact field performance. However, selection towards specific trait might result...

Abstract We identified females of six species leafhoppers (tribe Empoascini), Asymmetrasca decedens (Paoli), Empoasca affinis Nast, E. decipiens Paoli, pteridis (Dahlbom), vitis Göthe, and Jacobiasca lybica (Bergevin Zanon), using morphological characters the ovipositor isozyme mitochondrial COI sequence analyses. Useful traits are tooth morphology, length toothed area, form sculpturing, number position sensilla on first second pair valves. Among set enzyme systems assayed, four putative...

Biological control is occasionally supplemented with insecticides treatments, which may have a significant impact on natural enemies. Typically, selective compounds are chosen to overcome lack of compatibility, but an alternative approach the use biocontrol agents resistant pesticides. Orius laevigatus (Fieber) (Hemiptera: Anthocoridae) main predator used thrips and other small pests in greenhouses. The avermectin emamectin benzoate bioinsecticide developed for lepidopteran pests, reported...

Abstract Omnivores obtain resources from more than one trophic level, choosing food based on quantity and quality. They usually engage in intraguild predation (IGP) when prey is scarce. Orius laevigatus an example of omnivore that becomes superior predator the predatory mite Amblyseius swirskii released a combined system under low levels preferred prey, thrips Frankliniella occidentalis . Here, we test two genetically enhanced O. strains for bigger size better fitness feeding pollen (BIG30...

Con el objetivo de establecer posibles diferencias en uso tabaco, alcohol y drogas ilícitas entre mujeres hombres veracruzanos los diferentes momentos del confinamiento social la pandemia por COVID-19, durante periodo 2020 a 2022, se diseñó un estudio longitudinal. La información presente manuscrito forma parte proyecto Ansiedad, depresión, estilos vida bienestar contexto sociocultural actual, desarrollado Observatorio Calidad Vida Salud Social Universidad Veracruzana, donde contó con...

The fungus Fusarium oxysporum Sch.:Fr. presents a high biological and genetic variability, manifested by the existenceof many specialized forms races. With goal of evaluating relationship among different specializedforms, an experiment was carried out involving application two types molecular markers. One consistedof sequencing internal transcribed spacer (ITS) region ribosomal DNA 17 isolates belonging toseven forms: F.o. melonis (4 isolates), dianthi (2), niveum (4), lycopersici...