A5001497347- Advanced Proteomics Techniques and Applications
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Monoclonal and Polyclonal Antibodies Research
- Peptidase Inhibition and Analysis
- Viral Infections and Vectors
- Gene expression and cancer classification
- Trypanosoma species research and implications
- Advanced biosensing and bioanalysis techniques
- Toxoplasma gondii Research Studies
- Immune Cell Function and Interaction
- Tuberculosis Research and Epidemiology
- Bone Tumor Diagnosis and Treatments
- Extracellular vesicles in disease
- Molecular Biology Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Nanoplatforms for cancer theranostics
- Cancer Research and Treatments
- Research on Leishmaniasis Studies
- Immunotherapy and Immune Responses
- SARS-CoV-2 detection and testing
- Polymer Surface Interaction Studies
George Mason University
2016-2025
Fondazione IRCCS Istituto Nazionale dei Tumori
2016
Centro di Riferimento Oncologico
2007-2008
Istituti di Ricovero e Cura a Carattere Scientifico
2007-2008
National Cancer Institute
2007
University of Padua
2003-2006
University of Milano-Bicocca
2005
University of Perugia
2005
Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions minutes, one step, solution: (a) molecular size sieving, (b) capture all solution-phase target molecules, and (c) complete protection harvested from enzymatic degradation. The captured analytes can be readily...
The Mycobacterium tuberculosis –specific antigen lipoarabinomannan measured in the urine of HIV-negative, pulmonary TB-infected patients correlates with disease severity.
Many low-abundance biomarkers for early detection of cancer and other diseases are invisible to mass spectrometry because they exist in body fluids very low concentrations, masked by high-abundance proteins such as albumin immunoglobulins, labile. To overcome these barriers, we created porous, buoyant, core-shell hydrogel nanoparticles containing novel high affinity reactive chemical baits protein peptide harvesting, concentration, preservation fluids. Poly(N-isopropylacrylamide-co-acrylic...
Background The blood proteome is thought to represent a rich source of biomarkers for early stage disease detection. Nevertheless, three major challenges have hindered biomarker discovery: a) candidate exist at extremely low concentrations in blood; b) high abundance resident proteins such as albumin mask the rare biomarkers; c) are rapidly degraded by endogenous and exogenous proteinases. Methodology Principal Findings Hydrogel nanoparticles created with N-isopropylacrylamide based core...
Abstract Motivation: Microarray expression profiling appears particularly promising for a deeper understanding of cancer biology and to identify molecular signatures supporting the histological classification schemes neoplastic specimens. However, diagnostics based on microarray data presents major challenges due overwhelming number variables complex, multiclass nature tumor samples. Thus, development marker selection methods, that allow identification those genes are most likely confer high...
Over the past few years, mass spectrometry has emerged as a technology to complement and potentially replace standard immunoassays in routine clinical core laboratories. Application of protein peptide measurement can provide advantages including high sensitivity, ability multiplex analytes, specificity at amino acid sequence level. In our previous study, we demonstrated excellent reproducibility spectrometry-selective reaction monitoring (MS-SRM) assays when applying standardized operating...
Identifying the contact regions between a protein and its binding partners is essential for creating therapies that block interaction. Unfortunately, such are extremely difficult to characterize because they hidden inside interface. Here we introduce painting as new tool employs small molecules molecular paints tightly coat surface of protein-protein complexes. The paints, which trypsin cleavage sites, excluded from Following mass spectrometry, only peptides in interface emerge positive...
Prompt antibiotic treatment of early stage Lyme borreliosis (LB) prevents progression to severe multisystem disease. There is a clinical need improve the diagnostic specificity assays in period prior mounting robust serology response. Using novel analyte harvesting nanotechnology, Nanotrap particles, we evaluated urinary Borrelia Outer surface protein A (OspA) C-terminus peptide LB before and after treatment, patients suspected late disseminated LB. We employed particles concentrate OspA...
Urine is a potential source of diagnostic biomarkers for detection diseases, and very attractive means non-invasive biospecimen collection. Nonetheless, proteomic measurement in urine challenging because exist low concentration (usually below the sensitivity common immunoassays) may be subject to rapid degradation. Hydrogel nanoparticles functionalized with Cibacron Blue F3G-A (CB) have been applied address these challenges biomarker measurement. We chose one most difficult abundance, but...
Background Detection of congenital T. cruzi transmission is considered one the pillars control programs Chagas disease. Congenital accounts for 25% new infections with an estimated 15,000 infected infants per year. Current to detect disease in Latin America utilize microscopy early life and serology after 6 months. These suffer from low sensitivity by high loss follow-up later infancy. We developed a urine nanoparticle test (Chunap) concentrate, preserve antigens early, non-invasive...
Introduction: Mass spectrometry (MS) is the premier tool for discovering novel disease-associated protein biomarkers. Unfortunately, when applied to complex body fluid samples, MS has poor sensitivity detection of low abundance biomarkers (≪10 ng/mL), derived directly from diseased tissue cells or pathogens.Areas covered: Herein we discuss strengths and drawbacks technologies used concentrate analytes in fluids, with aim improve effective discovery. Solvent removal by dry-down dialysis,...
Despite much promise in overcoming drug-resistant infections, clinical studies of bacteriophage antibacterial therapy have failed to show durable effectiveness. Although lysogeny plays an important role bacterial physiology, its significance diverse microbiomes remains unclear. Here, we tested the following hypotheses: 1) urinary microbiome phage populations switch a higher relative proportion temperate phages, and 2) activity recombination machinery (integration/excision/transposition) is...
Background: Borreliosis infection and post-treatment Lyme disease syndrome are associated with nonspecific, multisystem symptoms, there is currently no effective direct test for disease. The seroprevalence of Borrelia miyamotoi among individuals suspected tick-borne illnesses persistent symptoms not well documented. Although bacterial extracellular vesicles (BEVs) have been suggested as potential sources biomarkers, evidence supporting their role in lacking. Methods: Patients (N=66) clinical...
Background: Melanoma represents only 4% of all skin cancers, but nearly 80% cancer deaths. This manuscript applies several new measurement technologies with the purpose elucidating molecular signatures melanoma aggressiveness. Purpose: We sought to determine whether low-abundant serum proteins related apoptotic pathways could be measured and correlated defined subtypes. Hydrogel core shell nanoparticles, a technology capable selectively entrapping low weight protecting them from enzymatic...
Abstract While lipopolysaccharides (LPS) induce dendritic cell (DC) maturation and migration to lymph nodes, glucocorticoids such as dexamethazone (Dex) have a profound suppressive effect on immune response. The mechanisms that might control this of Dex been extensively investigated in lymphocytes possible targets. Much less is known the effects DC, although they are recognized regulate immunity. To get insights into combined LPS DC functions, we undertaken genome‐wide analysis...
// Filippo Spreafico 1 , Italia Bongarzone 2 Sara Pizzamiglio 3 Ruben Magni 4 Elena Taverna Maida De Bortoli Chiara M. Ciniselli Barzanò Veronica Biassoni Alessandra Luchini Lance A. Liotta Weidong Zhou Michele Signore 5 Paolo Verderio and Maura Massimino Pediatric Oncology Unit, Department of Hematology Hematology-Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy Proteomics Laboratory, Experimental Molecular Medicine, Unit Medical Statistics, Biometry...