A5026750155- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Mitochondrial Function and Pathology
- Molecular Sensors and Ion Detection
- Cardiac Ischemia and Reperfusion
- Phytochemicals and Antioxidant Activities
- ATP Synthase and ATPases Research
- Redox biology and oxidative stress
- Chemical Synthesis and Analysis
- Crystallography and molecular interactions
- Porphyrin and Phthalocyanine Chemistry
- Cardiovascular Function and Risk Factors
- Supramolecular Chemistry and Complexes
- Cyclopropane Reaction Mechanisms
- Natural product bioactivities and synthesis
- Luminescence and Fluorescent Materials
- Synthetic Organic Chemistry Methods
- Photochromic and Fluorescence Chemistry
- Asymmetric Synthesis and Catalysis
- Photochemistry and Electron Transfer Studies
- Advanced Glycation End Products research
- Neuroscience and Neuropharmacology Research
- Click Chemistry and Applications
- Supramolecular Self-Assembly in Materials
- Electron Spin Resonance Studies
University of Glasgow
2013-2025
Glasgow Life
2021
Institut Químic de Sarrià
2013
Universitat Ramon Llull
2013
Durham University
2010
Université de Montpellier
2008
Engineering and Physical Sciences Research Council
2007
University of Manchester
2007
Mitochondrial oxidative stress, mitochondrial dysfunction, or both have been implicated in insulin resistance. However, disentangling the individual roles of these processes resistance has difficult because they often occur tandem, and tools that selectively increase oxidant production without impairing respiration lacking. Using dimer/monomer status peroxiredoxin isoforms as an indicator compartmental hydrogen peroxide burden, we provide evidence stress is localized to mitochondria...
Depolarization of an individual mitochondrion or small clusters mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix by alkyltriphenylphosphonium lipophilic cation and releases protonophore 2,4-dinitrophenol locally in predetermined regions response directed irradiation with UV light via local photolysis system. This also provides proof principle for general temporally spatially controlled release bioactive molecules, pharmacophores,...
[2-(14)C]quercetin-4'-glucoside (4 mg/kg body weight) was fed by gavage to rats housed in metabolic cages, and over an ensuing 72 h period, radiolabeled products tissues, plasma, feces, urine were monitored high-performance liquid chromatography with online radioactivity MS2 detection. One 6 after ingestion, while the small intestine, flavonol glucoside converted glucuronide methylated sulfated derivatives of quercetin, but only trace amounts these metabolites excreted urine. On entering...
Epidemiological studies suggest that consumption of flavonol-rich diets decreases the risk developing heart disease and certain cancers. Recent have detected flavonol conjugates in blood urine following various dietary interventions. To assess to what extent flavonols also accumulate tissues, where they might be expected exert anti-carcinogenic anti-atherogenic effects, [2-14C]quercetin-4'-glucoside was synthesized fed rats. After 60 min, 93.6% ingested radioactivity recovered from...
Mitochondrial glutathione (GSH) and thioredoxin (Trx) systems function independently of the rest cell. While maintenance mitochondrial thiol redox state is thought vital for cell survival, this was not testable due to difficulty manipulating organelle's those in other compartments. To overcome constraint we modified S-transferase substrate Trx reductase (TrxR) inhibitor, 1-chloro-2,4-dinitrobenzene (CDNB) by conjugation mitochondria-targeting triphenylphosphonium cation. The result,...
Coenzyme Q (CoQ) is an essential cofactor, primarily found in the mitochondrial inner membrane where it functions as electron carrier respiratory chain, and a lipophilic antioxidant. The redox state of CoQ pool ratio its oxidised (ubiquinone) reduced (ubiquinol) forms, key indicator bioenergetic antioxidant status. However, role vivo poorly understood, because determining value technically challenging due to changes during isolation, extraction analysis. To address these problems, we have...
Abstract Purpose Mitochondrial reactive oxygen species (ROS) production upon reperfusion of ischemic tissue initiates the ischemia/reperfusion (I/R) injury associated with heart attack. During ischemia, succinate accumulates and its oxidation by dehydrogenase (SDH) drives ROS production. Inhibition accumulation and/or dimethyl malonate (DMM), a cell permeable prodrug SDH inhibitor malonate, can decrease I/R injury. However, DMM is hydrolysed slowly, requiring administration to prior...
The transcription factor nuclear erythroid 2–related 2 (Nrf2) regulates the expression of genes involved in antioxidant defenses to modulate fundamental cellular processes such as mitochondrial function and GSH metabolism. Previous reports proposed that reactive oxygen species production disruption pool activate Nrf2 pathway, suggesting senses redox signals and/or oxidative damage nucleus respond appropriately. However, until now, it has not been possible disentangle overlapping effects...
Mitochondria-targeted H2S donors are thought to protect against acute ischemia-reperfusion (IR) injury by releasing that decreases oxidative damage. However, the rate of release current is too slow be effective upon administration following reperfusion. To overcome this limitation here we develop a mitochondria-targeted agent, MitoPerSulf very rapidly releases within mitochondria. quickly taken up mitochondria, where it reacts with endogenous thiols generate persulfide intermediate H2S....
Reactive oxygen species (ROS) have an equivocal role in myocardial ischaemia reperfusion injury. Within the cardiomyocyte, mitochondria are both a major source and target of ROS. We evaluate effects selective, dose-dependent increase mitochondrial ROS levels on cardiac physiology using mitochondria-targeted redox cycler MitoParaquat (MitoPQ). Low decrease susceptibility neonatal rat ventricular myocytes (NRVMs) to anoxia/reoxygenation injury also cause profound protection vivo mouse model...
Reviewing the literature from May 1998 to April 1999.
Quercetin-4′-glucoside is a major flavonol in onions, and this study investigated the absorption fate of radiolabeled quercetin-4′-glucoside rats. Rats ingested [2-<sup>14</sup>C]quercetin-4′-glucoside distribution radioactivity throughout body was determined after 0.5, 1, 2, 5 h. The gastrointestinal tract, liver, kidney, plasma were extracted, components identified quantified using high-performance liquid chromatography with on-line detection tandem mass spectrometry. Two hours dosing, all...
Male Sprague-Dawley rats ingested 140 × 10(6) dpm of [3-(14)C]trans-caffeic acid, and over the ensuing 72 h period, body tissues, plasma, urine, feces were collected overall levels radioactivity determined. Where sufficient had accumulated, samples analyzed by HPLC with online tandem mass spectrometric detection. Nine labeled compounds identified, substrate its cis isomer, 3'-O- 4'-O-sulfates glucuronides caffeic ferulic isoferulic acid-4'-O-sulfate. Four unidentified metabolites also...
Synergistic dipole-dipole and hydrogen-bonding interactions are used to assemble nanostructured materials. Precipitation of a donor-acceptor molecule 8-[[p-[bis(ethyl)amino]phenyl]azo]-isobutylflavin (ABFL) yields nanowires approximately 50-150 nm in diameter lengths several millimeters. the non-hydrogen-bonding analog, methylated ABFL (MABFL), generates micrometer-sized hexagonal platelets that 5-10 microm length, 1-5 width, 0.1-0.5 thick. The structural similarity two molecules allows...
Gold nanoparticles functionalised with a valine-derived formamide have been developed as effective homogenous catalysts for the asymmetric reduction of ketimine 1 trichlorosilane (≤84% ee) in toluene. This methodology both simplifies recovery catalyst and its separation from product, can be readily removed subsequently recycled by precipitation reaction mixture.
We describe the application of LCST a naphthalene-functionalised polyNIPAM derivative as convenient, tuneable and reversible method to disrupt complex formation with CBPQT(4+) in water.
Many mitochondrial metabolites and bioactive molecules contain two carboxylic acid moieties that make them unable to cross biological membranes. Hence, there is considerable interest in facilitating the uptake of these into cells mitochondria modify or report on their function. Conjugation triphenylphosphonium (TPP) lipophilic cation widely used deliver selectively response membrane potential. However, permanent attachment can disrupt function small dicarboxylates. Here, we have developed a...
The targeting of bioactive molecules and probes to mitochondria can be achieved by coupling the lipophilic triphenyl phosphonium (TPP) cation, which accumulates several hundred-fold within in response mitochondrial membrane potential (Δψ
Naphthoxanthenyl 1 is a new stable phenalenyl-type radical. Electrochemical studies indicate that has two reversible redox processes occur on comparatively short time scales. Crystals containing can be grown by electrocrystallization, suggesting they are conductive.