The choice of effective biocides used for routine hospital practice should consider the role disinfectants in maintenance and development local resistome how they might affect antibiotic resistance gene transfer within microbial population. Currently, there is little understanding different contribute to eDNA release that may subsequent environmental retention. Here, we investigated from mature biofilms two opportunistic model strains Pseudomonas aeruginosa ATCC 27853 (PA) Staphylococcus...

Collagen and its derivates are typically obtained by extracting them from fresh animal tissues. Lately, however, there has been an increased interest in obtaining collagen other sources, such as waste material, because of the growing trend to replace synthetic materials with sustainable, natural counterparts various industries, well ensure a rational revalorization. In this paper, was non-tanned leather production, taken at different stages production process: limed pelt, delimed fleshings....

Novel antibiotic combinations may act synergistically to inhibit the growth of multidrug-resistant bacterial pathogens but predicting which combination will be successful is difficult, and standard antimicrobial susceptibility testing not identify important physiological differences between planktonic free-swimming biofilm-protected surface-attached sessile cells. Using a nominally macrolide-resistant model Klebsiella pneumoniae strain (ATCC 10031) we demonstrate effectiveness several...

Pseudomonas fluorescens SBW25 is a model soil- and plant-associated bacterium capable of forming variety air–liquid interface biofilms in experimental microcosms on plant surfaces. Previous investigations have shown that cellulose the primary structural matrix component robust well-attached Wrinkly Spreader biofilm, as well fragile Viscous Mass biofilm. Here, we demonstrate both include extracellular DNA (eDNA) which can be visualized using confocal laser scanning microscopy (CLSM),...

Background: The present research has been undertaken to study the therapeutic potential of mesenchymal stem cells (MSCs) for treatment neuroinflammation-induced cognitive disorders. Methods: Either umbilical cord or adipose MSCs were injected into mice treated with lipopolysaccharide. studied in behavioral tests, and their brains examined by means immunohistochemistry, electron microscopy sandwich ELISA. Results: MSCs, introduced either intravenously intraperitoneally, restored episodic...

A therapeutic combination of azithromycin (AZM) and colistin methanesulfonate (CMS) was shown to be effective against both non-PDR PDR Klebsiella pneumoniae biofilms in vitro. These anti-biofilm effects, however, may not correlate with effects observed standard plate assays, nor will they representative vivo action. After all, biofilm-associated infection processes are also impacted by the presence wound bed components, such as host cells or fluids, which can all affect antibiotic...

Mesenchymal stem cells (MSCs) are ideal candidates for cell-based therapy aimed at tissue repair and immunomodulation.Aim.To study the survival of transplanted human MSCs from umbilical cord Wharton's Jelly (hWJ-MSCs) in animal model experimental osteoarthritis (OA) rats after injecting into a knee joint to explore effect collagen scaffold on cell vivo.Methods.MSC isolation cultivation vitro.Immunological phenotyping propagated hWJ-MSCs was performed by fl ow cytometry.The retention studied...

Aim.To develop the porous functionalized collagen scaffold for delivery of FGF-2 and studying its properties in vitro vivo.Methods.Porous scaffolds were prepared by freeze-drying I solutions containing polymer developed on basis cross-linked modified heparin.The have been analyzed SEM, AFM SCLM.The angiogenic activity these loaded with was tested a CAM assay.Results.The data obtained SEM SCLM analysis revealed that mainly has layered structure pores forming connection between layers.The...

Aim.To study the influence of melatonin and recombinant human fibroblast growth factor (rhFGF-2) on umbilical cord multipotent mesenchymal stromal cells (hMMSCs) effects at experimental demyelination.Methods.Adult mice were fed with neurotoxin cuprizone for 3 weeks.hMMSCs (5×10 5 cells) injected 10th day diet.Injections or rhFGF-2 started 11th diet.We used cell culture, flow cytometric, spectrophotometric histological methods, "open field" "rotarod" tests.Results.Under motor-, emotional...

Purpose. To develop a protocol for mesenchymal stem cells (MSCs) obtaining from wharton’s jelly of human umbilical cord, to study their phenotypic changes and proliferative potential in culture.Materials methods. MSCs cord were cultured 6 passages. morphological features the culture we used adapted staining with eosin gematoksilіn, Romanovsky–Giemsa, toluidine blue. For nuclear DNA coloring intercalators: Hoechst 33342, DARІ Ethidiumbromid. surface markers (CD105, CD90, CD73, CD34)...

Novel antibiotic combinations may act synergistically to inhibit the growth of multidrug-resistant bacterial pathogens but predicting which will be successful is difficult, and standard antimicrobial susceptibility testing not identify important physiological differences between planktonic free-swimming biofilm-protected surface-attached sessile cells. Using a nominally macrolide-resistant model Klebsiella pneumoniae strain (ATCC 10031) we demonstrate effectiveness several macrolides in...

Клеточная линия СНО

Aim: The aim of this work was to study the effects extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) on inflammation-impaired cognitive functions and brain mice. Methods: Young mice (~3-month-old) aged (~18-month-old) were injected with bacterial lipopolysaccharide (LPS) obtained intravenously donor 106 human umbilical cord MSCs, EVs isolated a similar amount MSCs or conditioned medium (CM) MSCs. Subsequently, examined in behavioral tests mouse brains analyzed for...

Abstract Digital light processing (DLP) is emerging as a powerful tool for fabricating tissue engineering (TE) scaffolds, particularly vascular TE and the development of representative in vitro wall models. For latter, biomaterials should mimick biological mechanical properties native blood vessels. To fabricate tubular constructs, DLP‐printing process optimized by exploiting acrylate‐endcapped urethane‐based (AUP) polymers presence acrylate end groups render them suitable DLP printing...

The development of a DNA-vaccine against classical swine fever (CSF) is perspective direction, because it gives an opportunity to develop marker vaccine due use part protective antigen molecule, and induce effectively both cellular humoral immune response.In this study recombinant plasmid, containing the fragment E2 gene CFS virus (CSFV) in eukaryotic expression vector, has been developed.It demonstrated that protein CSFV expressed CHO-K1 cells from developed plasmid pTR-BKneo -, we suggest...

Розробка ДНК-вакцин є одним з найперспективніишх напрямків у сучасній вакцинології. Проте, незважаючи на швидкі темпи розвитку цього напрямку в прикладному аспекті, недостатньо вивченим залишається широке коло питань щодо базових механізмів індукції імунної

Abstract Mesenchymal stem cells are the most promising regenerative medicine tool for treatment of various diseases, including liver disease, although exact mechanism their therapeutic action remains unclear. It was found that MSCs captured by lungs after systemic transplantation, quickly disappear, and not detected at site injury, but same time exhibit an obvious effect. Comparison MSC efficiency depending on route administration may shed light mechanisms involved in implementation...

The choice of effective biocides used for routine hospital practice should consider the role disinfectants in maintenance and development local resistome how they might affect antibiotic resistance gene transfer within microbial population. Currently, there is little understanding different contribute to eDNA release that may subsequent environmental retention. Here we investigated affected from mature biofilms two opportunistic model strains Pseudomonas aeruginosa ATCC 27853 (PA)...

The effect of transplantation umbilical cord-derived multipotent mesenchymal stromal cells (UC-MMSCs) to patients with demyelinating diseases depends on the age recipient and can change under influence hormones or growth factors. Purpose. To investigate exogenous melatonin recombinant human fibroblast factor-2 (rhFGF-2) effects UC-MMSCs transplanted into aged mice an experimental model multiple sclerosis. Material methods. 129/Sv mice, 15-17 months old, received neurotoxin cuprizone food for...

Keywords: Interferon-β1b, single-chain antibodies, immunoaffinity purification, cellulose-binding domain.

Aim.The study aims to determine the effects of transplantation preconditioned human umbilical cord mesenchymal stem cells (hUC-MSC) seeded on a biomimetic 3D scaffold.Methods.Porous collagen scaffolds were prepared by freeze-drying type I solutions.hUC-MSC isolated explant method.The hUC-MSC with H 2 O or LPS for 24 hours, and onto scaffolds.The MSC preconditioning studied xenogenic model.The five days after implantation analyzed histologically PCRbased detection human-specific sequences in...

Keywords: marker DNA-vaccine, booster immunization, humoral immune response, classical swine fever virus