A5032613589- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Mitochondrial Function and Pathology
- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Anesthesia and Neurotoxicity Research
- Neurological Disease Mechanisms and Treatments
- Biochemical effects in animals
- Ion channel regulation and function
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- Alzheimer's disease research and treatments
- Glycosylation and Glycoproteins Research
- Neurological Disorders and Treatments
- Mesenchymal stem cell research
- S100 Proteins and Annexins
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Pluripotent Stem Cells Research
- Diet and metabolism studies
- Epilepsy research and treatment
- Nerve injury and regeneration
- Nicotinic Acetylcholine Receptors Study
Bogomoletz Institute of Physiology
2015-2025
National Academy of Sciences of Ukraine
2005-2024
Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine
2014-2024
National Scientific Center "M.D. Strazhesko Institute of Cardiology"
2023
Institute of Molecular Biology and Genetics
2011-2014
University of Copenhagen
2004-2005
The Open University
1997
BioElectronics (United States)
1992
Oles Honchar Dnipro National University
1992
Institute of Evolutionary Physiology and Biochemistry
1978
Abstract Patterns of activity that induce synaptic plasticity at excitatory synapses, such as long‐term potentiation, result in structural remodeling the postsynaptic spine, comprising an enlargement spine head and reorganization density (PSD). Furthermore, synapses represent complex functional units which interaction between presynaptic varicosity is also modulated by surrounding astroglial processes. To investigate how patterns could affect morphological interplay these three partners, we...
Transplanted neurons derived from stem cells have been proposed to improve function in animal models of human disease by various mechanisms such as neuronal replacement. However, whether the grafted receive functional synaptic inputs recipient's brain and integrate into host neural circuitry is unknown. Here we studied cortical induced pluripotent after transplantation stroke-injured rat cerebral cortex. Using rabies virus-based trans-synaptic tracing method immunoelectron microscopy,...
Significance Many stroke patients exhibit long-term disability. Transplantation of stem cells has been proposed as a novel therapeutic strategy to promote recovery. However, little is known about whether these can form functionally integrated connections with neurons in the stroke-injured recipient’s brain. Here we show extensive morphological and functional integration axonal projections from intracortically transplanted human iPS cell–derived cortical brains rats ischemic lesions cerebral...
The neural cell adhesion molecule (NCAM) plays a critical role in development and plasticity of the nervous system is involved mechanisms learning memory. Here, we show that intracerebroventricular administration FG loop (FGL), synthetic 15 amino acid peptide corresponding to binding site NCAM for fibroblast growth factor receptor 1 (FGFR1), immediately after training rats fear conditioning or water maze learning, induced long-lasting improvement In primary cultures hippocampal neurons, FGL...
Relatively mild ischemic episode can initiate a chain of events resulting in delayed cell death and significant lesions the affected brain regions. We studied early synaptic modifications after brief ischemia modeled rats by transient vessels' occlusion vivo or oxygen–glucose deprivation vitro hippocampal CA1 pyramidal cells. Electron microscopic analysis excitatory spine synapses stratum radiatum revealed rapid increase postsynaptic density (PSD) thickness length, as well formation concave...
Several neurodegenerative diseases cause loss of cortical neurons, leading to sensory, motor, and cognitive impairments. Studies in different animal models have raised the possibility that transplantation human neuronal progenitors, generated from pluripotent stem cells, might be developed into a novel therapeutic strategy for disorders affecting cerebral cortex. For example, we shown long-term neuroepithelial-like (lt-NES) cell-derived produced induced cells transplanted stroke-injured...
Abstract Accumulating evidence suggests that extracellular matrix (ECM) molecules play important roles in formation of synapses. Our previous electrophysiologic study mice deficient the glycoprotein tenascin‐R (TN‐R) showed an impaired γ‐aminobutyric acid release at perisomatic inhibitory synapses CA1 pyramidal cell layer hippocampus. The present investigated possible ultrastructural correlates abnormal inhibition. Topographic, morphometric, and stereologic methods were applied light...
Lingonberries (LB) have been shown to beneficial metabolic effects, which is associated with an altered gut microbiota. This study investigated whether the LB-induced improvements were gut- and neuroinflammatory markers, as well cognitive performance in ApoE−/− mice fed high-fat (HF) diets. Whole LB, two separated fractions of LB investigated. Eight-week-old male HF diets (38% kcal) containing whole (wLB), or insoluble (insLB) soluble (solLB) for 8 weeks. Inclusion wLB insLB fraction reduced...
Abstract Background Periodontitis is a chronic inflammatory disease defined by the pathologic loss of periodontal ligament and alveolar bone in relation to aging. Although clinical cohort studies reported that periodontitis significantly elevated males compared females, emerging evidence indicates females with dementia are at greater risk for decreased bone. Objective This study aimed evaluate whether potential sex‐dependent factor using an experimental model induced triple transgenic...
There is a major unmet need for development of innovative strategies neuroprotection against ischemic brain injury. Here we show that FGL, neural cell adhesion molecule (NCAM)-derived peptide binding to and inducing phosphorylation the fibroblast growth factor receptor (FGFR), acts neuroprotectively after an insult both in vitro vivo. The neuroprotective activity FGL was tested on dissociated rat hippocampal neurons slice cultures, using protocol oxygen-glucose deprivation (OGD). protected...
Alzheimer's disease (AD)-linked protein, presenilin 1 (PS1), is present at the synapse, and knock-out of in mice leads to synaptic dysfunction. On other hand, activity was shown influence PS1-dependent generation distinct amyloid β (Aβ) species. However, precise nature these regulations remains unclear. The current study reveals novel role PS1 deciphers how vesicle-associated synaptotagmin (Syt1) modulate each functions neurons via direct activity-triggered interaction. Additionally,...
Cysteine proteases obtained from the stem of pineapple or papaya latex, bromelain and papain, respectively, exhibit a broad spectrum beneficial effects on human health. However, their gut microbiota composition dose-manner intestinal integrity healthy tissue have not been evaluated. In this study, C57BL/6 young, mice were fed papain in dose 1 mg per animal/day for three consecutive days, followed by assessment digestive protein capacity, morphology composition. Furthermore, reconstructed 3D...
Neuronal loss and axonal demyelination underlie long-term functional impairments in patients affected by brain disorders such as ischemic stroke. Stem cell-based approaches reconstructing remyelinating neural circuitry, leading to recovery, are highly warranted. Here, we demonstrate the vitro vivo production of myelinating oligodendrocytes from a human induced pluripotent stem cell (iPSC)-derived neuroepithelial (lt-NES) line, which also gives rise neurons with capacity integrate into...
ABSTRACT Intracerebral transplantation of stem cell-derived oligodendrocytes (OLs) holds promise as a new strategy for repairing demyelinated brain tissue. However, two challenges hinder clinical translation: the slow and inefficient generation human OLs using protocols described to date, limited insight into their remyelination potential, which has only been evaluated in vitro or xenotransplantation studies, failing capture critical human-specific cellular interactions involved myelination....
Abstract Stem/progenitor cell-based therapies hold promises for repairing the damaged nervous system. However, efficiency of these approaches neuronal replacement remains very limited. A major challenge is to develop pretransplant cell manipulations that may promote survival, engraftment, and differentiation transplanted cells. Here, we investigated whether overexpression fibroblast growth factor-2 (FGF-2) in grafted neural progenitors could improve their integration host tissue. We show...