A5031840294- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Calcium signaling and nucleotide metabolism
- PI3K/AKT/mTOR signaling in cancer
- Lysosomal Storage Disorders Research
- Pancreatic function and diabetes
- Protein Kinase Regulation and GTPase Signaling
- Endoplasmic Reticulum Stress and Disease
- Retinal Development and Disorders
- Advanced Fluorescence Microscopy Techniques
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Lipid metabolism and biosynthesis
- Microtubule and mitosis dynamics
- Morphological variations and asymmetry
- Sirtuins and Resveratrol in Medicine
- Polyamine Metabolism and Applications
- Cell Image Analysis Techniques
- Genetics and Neurodevelopmental Disorders
- Cholesterol and Lipid Metabolism
- Lipid Membrane Structure and Behavior
- Genetics, Aging, and Longevity in Model Organisms
- Hippo pathway signaling and YAP/TAZ
- Neuroscience and Neuropharmacology Research
University of California, Berkeley
2016-2025
Innovative Genomics Institute
2022-2024
Berkeley College
2022-2024
QB3
2016-2022
Whitehead Institute for Biomedical Research
2010-2015
Allen Institute
2010-2015
Massachusetts Institute of Technology
2010-2015
Broad Institute
2011-2015
Howard Hughes Medical Institute
2002-2015
Koch Institute for Integrative Cancer Research At MIT
2010-2012
The mTOR complex 1 (mTORC1) protein kinase is a master growth regulator that stimulated by amino acids. Amino acids activate the Rag guanosine triphosphatases (GTPases), which promote translocation of mTORC1 to lysosomal surface, site activation. We found vacuolar H(+)-adenosine triphosphatase ATPase (v-ATPase) necessary for mTORC1. v-ATPase engages in extensive acid-sensitive interactions with Ragulator, scaffolding anchors GTPases lysosome. In cell-free system, ATP hydrolysis was regulate...
Sensing amino acids at the lysosome The mTORC1 protein kinase is a complex of proteins that functions to regulate growth and metabolism. Activity sensitive abundance acids, but how sensing coupled control has been unclear. Wang et al. searched for predicted membrane interacted with regulators mTORC1. They identified currently known only as SLC38A9. Interaction SLC38A9 was presence acids. sequence similarity acid transporters. Effects modulation in cultured human cells indicate it may be...
Stem cells can sort mitochondria by age The renewal of tissues in aging organisms requires stem cells, which have the unusual ability to divide asymmetrically into one daughter cell that retains properties and another differentiates a particular tissue type. Katajisto et al. used photoactivated marker proteins monitor organelles stemlike from human breast their distribution cells. Most were evenly distributed, but maintained stem-cell received more newly produced fewer old ones. Science ,...
The mechanistic target of rapamycin complex 1 (mTORC1) protein kinase is a master growth regulator that becomes activated at the lysosome in response to nutrient cues. Here, we identify cholesterol, an essential building block for cellular growth, as input drives mTORC1 recruitment and activation lysosomal surface. transmembrane protein, SLC38A9, required by cholesterol through conserved cholesterol-responsive motifs. Moreover, SLC38A9 enables independently from its arginine-sensing...
A transcriptional regulatory mechanism enables cellular adaptation to nutrient availability and supports cancer metabolism.