A5102866101- Prenatal Screening and Diagnostics
- Hematopoietic Stem Cell Transplantation
- Cardiac Ischemia and Reperfusion
- Genomic variations and chromosomal abnormalities
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Monoclonal and Polyclonal Antibodies Research
- Effects of Radiation Exposure
- Pregnancy and preeclampsia studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Fetal and Pediatric Neurological Disorders
- Cardiac electrophysiology and arrhythmias
- Reproductive Biology and Fertility
- RNA Interference and Gene Delivery
- T-cell and B-cell Immunology
- Hedgehog Signaling Pathway Studies
- Immune Cell Function and Interaction
- Genetic Syndromes and Imprinting
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Assisted Reproductive Technology and Twin Pregnancy
- Sexual Differentiation and Disorders
- Receptor Mechanisms and Signaling
- NF-κB Signaling Pathways
- Hemoglobinopathies and Related Disorders
Samford University
2019-2023
University of Vermont
2010-2022
University of Vermont Medical Center
2021
St Vincent’s Private Hospital Sydney
2020
University of Kentucky
2001-2016
Lexington VA Health Care System
1993-2016
John Wiley & Sons (United States)
2016
Henry Ford Health System
2014
Veterans Health Administration
2008-2010
Tomsk State Pedagogical University
2010
An understanding of Al3+-induced diseases requires identification the blood carrier Al3+ to tissues where exerts a toxic action. Quantitative studies demonstrate that protein transferrin (iron-free) is strongest binder in plasma. Under plasma conditions pH 7.4 and [HCO3-]27 mmol/L, successive stability constant values for binding are log K1 = 12.9 K2 12.3. When concentration total 1 mumol/L, free permitted by 10(-14.6) mol/L, less than allowed insoluble Al(OH)3, Al(OH)2H2PO4, or complexing...
Natural killer (NK) cells play a central role in immune surveillance against tumors and viral infections. NK activity is depressed patients who have wide range of carcinomas, including carcinomas the lung. Peripheral blood was measured 22 nonsmokers, 15 light/moderate smokers, 12 heavy 19 with carcinoma Patients lung had marked depression compared nonsmokers. Light/moderate smokers comparable to that whereas These results suggest smoking-induced alterations may pathogenesis smoking-associated
The structurally related cell adhesion molecules L1 and Nr-CAM have overlapping expression patterns in cerebellar granule cells. Here we analyzed their involvement development using mutant mice. Nr-CAM–deficient cells failed to extend neurites vitro on contactin, a known ligand for expressed the cerebellum, confirming that these mice are functionally null Nr-CAM. In vivo, Nr-CAM–null cerebella did not exhibit obvious histological defects, although mild size reduction of several lobes was...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTExpression of the amino-terminal half-molecule human serum transferrin in cultured cells and characterization recombinant proteinWalter D. Funk, Ross T. A. MacGillivray, Anne B. Mason, Stephen Brown, Robert C. WoodworthCite this: Biochemistry 1990, 29, 6, 1654–1660Publication Date (Print):February 13, 1990Publication History Published online1 May 2002Published inissue 13 February...
The immune status of 17 healthy individuals 100-103 years age (centenarians) was investigated. Qualitative values for immunoglobulins IgG, IgM, IgA, and IgE were within normal ranges subjects more than 60 with the exception elevated IgM in one individual. Cell marker studies employing a panel 27 monoclonal antibodies delineating T B lymphocytes, monocytes, natural killer cells, granulocytes, functional developmental subsets each performed to phenotype peripheral blood leukocytes. Although...
Heterozygous loss-of-function mutations in ZIC2 result the severe brain malformation known as holoprosencephaly (HPE), indicating that forebrain development is exquisitely sensitive to activity of this poorly understood transcription factor. To identify regions are essential for activity, we have assessed ability a variety mutant proteins function vitro assays. Two sources information were used design relevant mutations. First, phenotype producing human and mouse mimicked secondly,...
Mutations in the Adenomatous Polyposis Coli ( APC ) tumor suppressor gene or β- catenin are present most colon cancers and less frequently other types. In this study, we screened 24 human breast cancer cell lines three immortalized epithelial for alterations γ-catenin by western blotting, protein truncation assay DNA sequence analysis. one line (DU 4475), an mutation was identified (E1577stop) that resulted expression of truncated APC. This associated with elevated cytosolic β-catenin...
Purpose : To investigate the role of NF- κB1 (p50) gene in ionizing radiation (IR)-induced κB activation and TNF α, IL-1 β IL-6 mRNA expression vivo. Materials methods was analysed by gel shift/supershift assay levels were measured using RNase protection (RPA). Various tissues from BALB/c, B6,129P-Nfkb1 (NF- or p50 knockout, -/-) B6,129PF2 (wild-type, +/+) mice before after exposure to a lethal dose (8.5 Gy) total-body γ-irradiation. Results Exposure BALB/c IR selectively activated spleen,...
T10B9.1A-31, a nonmitogenic immunoglobulin Mk monoclonal antibody that detects an epitope on the alpha/beta chains of T cell antigen receptor (TCRα/β), or OKT3, anti-CD3 mAb, was employed in randomized double-blind phase II clinical trial to treat biopsyproven acute cellular renal allograft rejection. Two 40 patients initially selected for protocol were considered be nonevaluable. Analysis remaining 38 receiving both living related and cadaveric donor allografts revealed patient survival...
Abstract Exposure of mice to total body irradiation induces nuclear factor κB (NFκB) activation in a tissue-specific manner. In addition the spleen, lymph nodes, and bone marrow, tissues that exhibit NFκB now include newly identified site intestinal epithelial cells. activated by mainly consists p50/RelA heterodimers, genetically targeted disruption p50 gene significantly decreased activation. By comparing tissue damage lethality wild-type knockout (p50−/−) after they were exposed increasing...
Although assisted reproductive technology (ART) is reported to result in abnormal genomic imprinting and/or altered methylation, few if any studies have used high-throughput methods analyze methylation ART embryos. We hypothesized that a microarray-based assessment of could be reveal differences between and normal preimplantation In this pilot study, we performed methylation-sensitive amplification DNA from mouse blastocysts, obtained by natural mating either maintained vivo until E3.5 (n=...