A5067097625- Effects of Radiation Exposure
- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Radiation Therapy and Dosimetry
- Radiation Dose and Imaging
- Clusterin in disease pathology
- Radioactive contamination and transfer
- Biomarkers in Disease Mechanisms
- Cancer-related Molecular Pathways
- Plant Genetic and Mutation Studies
- Spaceflight effects on biology
- Telomeres, Telomerase, and Senescence
- Genomics and Chromatin Dynamics
- Cancer, Hypoxia, and Metabolism
- FOXO transcription factor regulation
- Ferroptosis and cancer prognosis
- Radioactivity and Radon Measurements
- CRISPR and Genetic Engineering
- Electromagnetic Fields and Biological Effects
- Anesthesia and Neurotoxicity Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Epigenetics and DNA Methylation
- Health, Environment, Cognitive Aging
- Molecular Biology Techniques and Applications
- Radiation Effects and Dosimetry
Autorité de Sûreté Nucléaire
2025
Institut de Radioprotection et de Sûreté Nucléaire
2020-2024
University of Ottawa
2017-2024
McGill University
2024
Canadian Nuclear Laboratories
2011-2019
Atomic Energy (Canada)
2010-2014
BC Cancer Agency
2010
Occupational Cancer Research Centre
2006
Institute of Theoretical and Experimental Biophysics
2000-2004
Case Western Reserve University
2003-2004
Nuclear clusterin (nCLU) is an ionizing radiation (IR)-inducible protein that binds Ku70, and triggers apoptosis when overexpressed in MCF-7 cells. We demonstrate endogenous nCLU synthesis a product of alternative splicing. Reverse transcriptase-PCR analyses revealed exon II, containing the first AUG encoding endoplasmic reticulum-targeting peptide, was omitted. Exons I III are spliced together placing downstream as available translation start site. This shorter mRNA produces 49-kDa...
Abstract Background Evidence suggests that tumor cells exposed to some DNA damaging agents are more likely die if they retain microscopically visible γH2AX foci known mark sites of double-strand breaks. This appears be true even after exposure the alkylating agent MNNG does not cause direct breaks but produce when damaged undergoes replication. Methods To examine this predictive ability further, SiHa human cervical carcinoma were 8 drugs (camptothecin, cisplatin, doxorubicin, etoposide,...
The linear no-threshold (LNT) model was introduced into the radiological protection system about 60 years ago, but this and its use in radiation are still debated today. This article presents an overview of results on effects exposure to low linear-energy-transfer radiobiology epidemiology accumulated over last decade discusses their impact LNT assessment radiation-related cancer risks at doses. knowledge acquired past 10 years, both epidemiology, has reinforced scientific In radiobiology,...
// Andreyan N. Osipov 1, 2, 3, 4 , Margarita Pustovalova 2 Anna Grekhova 5 Petr Eremin 1 Natalia Vorobyova 3 Andrey Pulin Alex Zhavoronkov 4, 6, 7 Sergey Roumiantsev 8 Dmitry Y. Klokov 9 Ilya State Research Center - Burnasyan Federal Medical Biophysical of Biological Agency (SRC-FMBC), Moscow, Russia Semenov Institute Chemical Physics, Russian Academy Sciences, Rogachev Pediatric Hematology, Oncology and Immunology, Moscow Physics Technology, Dolgoprudny, Region, Emanuel for Biochemical 6...
AbstractThe clusterin (CLU) protein has been reported to have both cytoprotective and cytotoxic activities. Previous data from our lab suggest that the secretory form of CLU (sCLU) is induced after very low, nontoxic doses ionizing radiation (IR: >0.02 Gy), while a nuclear cytotoxic.1 Cells must presumably suppress sCLU stimulate cell death, however, factors regulating stress-inducible expression not elucidated. Here we demonstrate p53 can induction responses. A variety agents stimulated DNA...
DNA double-strand breaks (DSB) are among the most harmful lesions induced by ionizing radiation (IR). Although induction and repair of radiation-induced DSB is well studied for acute irradiation, responses to produced chronic IR exposures poorly understood, especially in human stem cells. The aim this study was examine formation markers (γH2AX phosphorylated kinase ATM, pATM, foci) mesenchymal cells (MSCs) exposed gamma-radiation (0.1 mGy/min) comparison with irradiation (30 at cumulative...
At high exposure levels ionizing radiation is a carcinogen. Little known about how human stem cells, which are to contribute tumorigenesis, respond prolonged exposures. We studied formation of DNA double strand breaks, accessed as γH2AX and 53BP1 foci, in mesenchymal cells (MSCs) exposed either acute (5400 mGy/h) or (270 X-irradiation. show linear dose response for In contrast, resulted dose-response curve that had an initial portion followed by plateau. Analysis Rad51 marker homologous...
Development of personalized skin treatment in medicine and care may benefit from simple accurate evaluation the fraction senescent fibroblasts that lost their proliferative capacity. We examined whether enriched analysis colonies formed by primary human fibroblasts, a widely available cellular assay, could reveal correlations with cells heterogenic cell population. measured fractions senescence associated β-galactosidase (SA-βgal) positive either mass cultures or various morphological...
<title>Abstract</title> Throughout their lives, individuals are exposed to various pollutants, potentially including co-exposure radiological and chemical stressors. Yet, existing literature about these combinations is scarce. We selected tungsten ionizing radiations. Tungsten an emerging contaminant present as aerosolized particles in several scenarios, concurrently with low-dose irradiation, causing a co-exposure. The cerebral toxicity of this was studied after 24 hours 28 days the frontal...
Mechanisms underlying the effects of low-dose ionizing radiation (IR) exposure (10-100 mGy) remain unknown. Here we present a comparative study early (less than 24h) and delayed (up to 11 post-irradiation passages) caused by low (80 vs intermediate (1000 dose X-ray in cultured human bone marrow mesenchymal stem cells (MSCs). We show that γН2АХ foci induced an returned back control value 24 h post-irradiation. In contrast, irradiation resulted residual still at h. Notably, these were not...
// Andreyan N. Osipov 1, 2, 3, 4 , Anna Grekhova 5 Margarita Pustovalova 2 Ivan V. Ozerov 1 Petr Eremin Natalia Vorobyeva 3 Lazareva Andrey Pulin Alex Zhavoronkov 4, 6, 7 Sergey Roumiantsev 8 Dmitry Klokov 9 Ilya State Research Center - Burnasyan Federal Medical Biophysical of Biological Agency (SRC-FMBC), Moscow 123098, Russia Semenov Institute Chemical Physics, Russian Academy Sciences, 119991, Rogachev Pediatric Hematology, Oncology and Immunology, 117997, Physics Technology, Dolgoprudny,...
Le modèle linéaire sans seuil (LNT) a été introduit dans le système de radioprotection il y environ 60 ans, mais ce et son utilisation en sont encore débattus aujourd’hui. Cet article résume les résultats radiobiologie épidémiologie accumulés au cours la dernière décennie sur effets d’une exposition aux rayonnements ionisants à faible Transfert d’Énergie Linéique (TEL) discute leur impact l’utilisation du LNT l’évaluation des risques cancer par rayonnement faibles doses. Les connaissances...
While AI is widely used in biomedical research and medical practice, its use constrained to few specific practical areas, e.g., radiomics. Participants of the workshop on “Artificial Intelligence Biology Medicine” (Jerusalem, Feb 14–15, 2023), both researchers practitioners, aimed build a holistic picture by exploring advancements, challenges perspectives, as well suggest new fields for applications. Presentations showcased potential large language models (LLMs) generating molecular...