A5082076400- Reproductive tract infections research
- Sperm and Testicular Function
- Pelvic floor disorders treatments
- Urinary Tract Infections Management
- Gut microbiota and health
- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Preterm Birth and Chorioamnionitis
- Venous Thromboembolism Diagnosis and Management
- Oral microbiology and periodontitis research
- Indigenous Studies and Ecology
- Oral Health Pathology and Treatment
- HIV, TB, and STIs Epidemiology
- Cervical Cancer and HPV Research
- Reproductive Health and Technologies
- Healthcare Systems and Public Health
- Assisted Reproductive Technology and Twin Pregnancy
- Periodontal Regeneration and Treatments
- Pharmacological Effects and Assays
- Diphtheria, Corynebacterium, and Tetanus
- Pesticide Residue Analysis and Safety
- Gynecological conditions and treatments
- Glutathione Transferases and Polymorphisms
- Digital Transformation in Law
- Oral and gingival health research
Ural State Medical University
2018-2025
University Medical Center
2024
National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov of the Ministry of Healthcare of the Russian Federation
2023
Ekaterinburg Research Institute of Viral Infections
2023
Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2023
N.N. Krasovskii Institute of Mathematics and Mechanics of the Ural Branch of the Russian Academy of Sciences
2020
Background. Group B streptococcus (Streptococcus agalactiae, SGV) remains the main cause of neonatal sepsis and meningitis, despite a marked decrease due to use intranatal antibiotic prophylaxis. At same time, delays in detection treatment infections can serious consequences and, some cases, death newborn, on other hand, unnecessary antibiotics also has harmful (changes normal microbiota development antimicrobial resistance, etc.). laboratories are constantly faced with task improving...
Introduction . A 0.05% solution of chlorhexidine digluconate (CD) is one the most commonly used antiseptics, however it unclear whether CD has an inhibitory effect on nucleicacid amplification during polymerase chain reaction (PCR). The aim study To evaluate impact bacterial DNA preservation and PCR efficiency. Materials methods susceptibility Lactobacillus acidophilus, Staphylococcus aureus, Escherichia coli mixture to was studied after 1, 2, 7-day exposure in a refrigerator freezer....
Among other things male sterility can be caused by inflammatory diseases of the urogenital tract, often associated with opportunistic microorganisms. Thus, it is necessary to implement modern methods for detection and identification microorganisms in tract. The aim work was conduct comparative analysis ejaculate microbiota from men reproductive age studied using quantitative polymerase chain reaction (PCR) culture method. 86 samples collected aged 18–57 years after observing sexual...
Одной из причин мужского бесплодия могут быть воспалительные заболевания урогенитального тракта, развитие которых в ряде случаев ассоциировано с условно-патогенными микроорганизмами (УПМ). В связи этим актуальна проблема внедрения современных методов выявления и идентификации УПМ урогенитальном тракте. Целью работы было провести сравнительный анализ результатов исследования микробиоты эякулята мужчин репродуктивного возраста помощью количественной полимеразной цепной реакции (ПЦР) (тест...
To this day semen microbiota is still poorly understood, and clinical significance of detecting specific microorganism groups has not been clearly determined. The aim work was to conduct cluster analysis detected using real-time PCR. 634 samples reproductive age men were analyzed the Androflor kit. Microbial DNA in quantity no less than 103 GE/ml 460 (72.5%). From 1 14 350 (55.2%) quantities that exceeded threshold values (the detection rate groups: 3.3–21.0%). In these 4 stable clusters...
Mikrobiota eyakulyata do sikh por nedostatochno izuchena, a klinicheskaya znachimost' vyyavleniya otdel'nykh grupp mikroorganizmov okonchatel'no ne ustanovlena. Tsel'yu raboty bylo provesti klasternyy analiz mikrobioty eyakulyata, vyyavlennoy metodom PTSR-RV. S pomoshch'yu testa «Androflor» («DNK-Tekhnologiya») issledovali 634 obraztsa muzhchin reproduktivnogo vozrasta. Mikrobnuyu DNK v kolichestve meneye 103 GE/ml obnaruzhili 460 (72,5%) obraztsov. V 350 (55,2%) probakh vyyavili ot 1 14...
The analysis of semen microbiota is difficult due to the lack established criteria for interpretation microbiological tests. aim study was determine stable clusters analyzed by real-time PCR in samples with normozoospermia. Semen 227 men normal spermiograms were included study. quantity total bacterial DNA and at least one group microorganisms more than 103 GE/ml 107 (41.7%) samples. Four prevalence a specific microorganism distinguished these samples: obligate anaerobes (OA) cluster...
Relevance: the aims of study was to compare effectiveness use various systemic antibiotics in patients with aggressive generalized periodontitis. A comparative antibiotic therapy using modern molecular biological methods is important periodontology. Materials and methods: a prospective, randomized, controlled 112 periodontitis before after real-time PCR (GE/ml) major periodontal pathogens. The median chosen as quantitative measure central tendency. Upper lower quartiles were used for interval...
Comparative analysis of the results adjuvant therapy in 60 patients with HPV-associated cervical neoplasms. It is established that use local immunomodulatory Polyoxidonium before conducting destructive methods treatment improves state immunity, promotes normalization microflora vagina and elimination human papillomavirus, increases effectiveness therapy, reduces recurrence process.
Background : the increase in life expectancy of a modern person is accompanied by an prevalence neurocognitive disorders. Various indicators associated with biological age are consistent deficits. In process ontogeny, complex symbiotic relationship develops between host and microbe. Presumably, they realized along microbiota-gut-brain axis. The participation intestinal microbiota ontogeny brain assumed. purpose review based on systematic scientific literature, to summarize research data...
This study was conducted to compare the semen microbial composition using real-time PCR in patients with asthenozoospermia and normozoospermia. The assessed by (Androflor kit, DNA-Technology, Russia) 242 (AS) 377 normozoospermia (NS). DNA (as total bacterial load, TBL) quantity of at least 103 GE/ml detected 268 (71.1 %) samples NS 185 (76.4 AS. According cluster analysis, microbiota positive represented one eight stable clusters predominance a certain group bacteria. In dominated...
Introduction. Chronic endometritis (CE) is reported in 12% of patients with primary infertility. Herpesviruses are considered as one the causes CE. The aim study. To evaluate frequency herpesviruses DNA detection endometrium, cervix and vagina chronic endometrial hyperplasia. Materials methods. 167 women reproductive age were included Samples from posterolateral wall vagina, endometrium taken each patient. obtained samples analyzed by real-time PCR for presence Herpes simplex viruses type 1...
The effectiveness of the use PRESIDENT ADHESIUM gum balm in adolescents with chronic gingivitis was studied according to clinical and cytological data. Materials methods. A open non-comparative study treatment generalized catarrhal (ICD-10 code K05.1) conducted. Thirty-eight children participated study, average age 14,9 years. Clinical dental examination included determination caries intensity DMF, hygiene index OHI-S, gingival inflammation PMA (modified by Parma), sulcus bleeding PBI...
Chronic endometritis (CE) in women of the reproductive age is associated with infertility and recurrent pregnancy loss. The aim this study was to evaluate endometrial microbiota by means real-time PCR reproductive-age depending on morphological pattern endometrium. Using Androflor kit, we analyzed aspirate collected from 23 patients chronic endometritis, 30 hyperplasia, 19 healthy women. DNA up 9 groups microorganisms detected all samples amounts exceeding negative control. total bacterial...
Odin iz klyuchevyh faktorov nastupleniya beremennosti pri ispol'zovanii vspomogatel'nyh reproduktivnyh tekhnologij (VRT) — poluchenie dostatochnogo kolichestva embrionov horoshego i otlichnogo kachestva. Cel'yu raboty bylo razrabotat' matematicheskuyu model' dlya prognozirovaniya kachestva poluchaemyh na osnovanii rezul'tatov ocenki mikrobnogo sostava eyakulyata, ispol'zuemogo oplodotvoreniya v programmah VRT normozoospermii. Eyakulyat 127 muzhchin ispol'zovali procedure ekstrakorporal'nogo...
АНАЛИТИЧЕСКИЕ ОБЗОРЫ Фундаментальные основы современных подходов к оценке микробиоты кишечника детейМикробиоту у детей рассматривают как один из жизненно важных экстракорпоральных органов, определяющих здоровье человека в детском и взрослом возрасте.В течение первых лет жизни состав кишечной претерпевает значительные изменения, на которые влияет множество факторов.Изменения составе взаимосвязаны с развитием заболеваний желудочно-кишечного тракта разнообразной инфекционной неинфекционной...
Aim. To compare the species composition of lactobacilli in women reproductive age using real time PCR on urogenital samples before and after culturing microorganisms Blood-Serum-Y^ast extract-Agar (BSYA). Materials methods. Using PCR, we have examined vaginal 25 healthy age. Samples swabs (endocervical swabs) were studied. Species identification has been carried out twice: native clinical material received microorganism culture technique BSYA. Results. After BSYA, L. iners predominance was...
Ocenka mikrobioty eyakulyata oslozhnena iz-za otsutstviya chetkih kriteriev dlya interpretacii mikrobiologicheskih testov. Cel'yu raboty bylo opredelit' ustojchivye varianty mikrobioty, issledovannoj metodom PCR-RV, v obrazcah s normozoospermiej. V issledovanie vklyuchili 227 prob eyakulyata, otvechayushchih kriteriyam normozoospermii. 107 (41,7%) fiksirovali nalichie summarnoj bakterial'noj DNK i hotya by odnoj iz issledovannyh grupp mikroorganizmov znacheniyah ne menee 103 GE/ml. dannyh...