Abstract The aim of this work is to evaluate the impact on rat microbiota long‐term feeding with phenolic compounds (PC) rich grape pomace extracts. Thirty, 2‐mo‐old rats, were divided into 5 groups. Four groups treated different concentrations PC (2.5, 5, 10, and 20 mg/kg/d diluted in 0.1% DMSO), 1 group received Dimethyl Sulfoxide (DMSO) alone (control group). daily treatment lasted 14 mo. Major constituents characterized by high‐performance liquid chromatography free radical scavenging...

Circadian rhythm disruption is increasingly considered an environmental risk factor for the development and exacerbation of inflammatory bowel disease. We have reported in a previous study that nychthemeral dysregulation associated with increase intestinal barrier permeability inflammation mice dextran sulfate sodium (DSS)-induced colitis. To investigate effect circadian on composition diversity gut microbiota (GM), sixty male C57BL/6J were initially divided to two groups, shifted group (

To detect, characterize, and assess the genetic clonality of carbapenem-non-susceptible Enterobacteriaceae in 10 Lebanese hospitals 2012.Selected isolates with reduced susceptibility to carbapenems were subject phenotypic study including antibiotic susceptibility, cloxacillin effect, modified Hodge test, activity efflux pump inhibitor. Carbapenemase genes detected using PCR; clonal relatedness was studied by pulsed field gel electrophoresis.Out 8717 isolated 2012, 102 (1.2%) showed...

The cardiovascular effects of mild and overt thyroid disease include a vast array pathological changes. As well, replacement therapy has been suggested for preserving cardiac function. However, the influence hormones on remodeling not thoroughly investigated at molecular cellular levels. purpose this paper is to study effect hypothyroidism alterations. Thirty Wistar rats were divided into 2 groups: control (n = 10) group treated with 6-propyl-2-thiouracil (PTU) 20) induce hypothyroidism. Ten...

Human noroviruses are highly contagious pathogens that cause acute infectious gastroenteritis. The high frequency of mutational variability causes the formation a wide range new genovariants. In Russian Federation, incidence rate norovirus infection in 2023 was 34.5, while Sverdlovsk region is one subjects Federation with highest rate. objective study to investigate genetic diversity and conduct phylogenetic analysis isolates for period 2022–2024. Materials methods. Biological samples native...

The establishment and development of the intestinal microbiota is known to be associated with profound short- long-term effects on health full-term infants (FTI), but studies are just starting for preterm (PTI). data also mostly come from western countries little information available Middle East. Here, we determined composition dynamics during first month life PTI (n = 66) FTI 17) in Lebanon. Fecal samples were collected weekly analyzed by quantitative PCR (q-PCR) temporal temperature...

Circadian rhythm disruption is increasingly considered an environmental risk factor for the development and exacerbation of inflammatory bowel disease. We have reported in a previous study that nychthemeral dysregulation associated with increase intestinal barrier permeability inflammation mice dextran sulfate sodium (DSS)-induced colitis. To investigate effect circadian on composition diversity gut microbiota (GM). Sixty male C57BL/6J were divided initially to two groups, shifted group...

This Lebanese study tested the hypothesis that differences would exist in gut microbiota of preterm infants with and without necrotising enterocolitis (NEC), as reported Western countries.This compared 11 NEC controls, all born at 27-35 weeks, three neonatal intensive care units between January 2013 March 2015. Faecal samples were collected key time points, was analysed by culture, quantitative PCR (qPCR) temperature temporal gel electrophoresis (TTGE).The cultures revealed poorly colonised...

Spirulina (Arthrospira platensis) is reported to play a role in improving nonalcoholic fatty liver disease (NAFLD) and intestinal microbiota (IM). To study spirulina’s effects the improvement of NAFLD characteristics, IM, pancreatic–renal lesions induced by fructose-enriched diet, 40 Wistar healthy male rats, weighing 200–250 g, were randomly divided into four groups 10, each rat per group was assigned diet equal quantities (20 g/day) for 18 weeks. The first control (CT) fed standardized...

Islets of Langerhans and β-cell isolation constitute routinely used cell models for diabetic research, refining islet protocols quality assessment is a high priority. Numerous have been published describing isolate islets, but often rigorous systematic their integrity lacking. Herein, we propose new protocol optimal generation islets. Pancreases from mice rats were excised digested using low-activity collagenase solution islets then purified by series sedimentations Percoll gradient....

Human noroviruses (HuNoVs) are highly contagious pathogens responsible of norovirus-associated acute gastroenteritis (AGE). GII.4 is the prevailing HuNoV genotype worldwide. Currently there no studies on molecular monitoring and phylogenetic analysis HuNoVs in territory Sverdlovsk region; therefore, it not possible to objectively assess their genetic diversity. The aim study carry out genotyping region from 2022 2023. Fecal samples (

Human non-polio enteroviruses (NPEVs) are the etiological agents involved in most cases of hand-foot-and-mouth disease (HFMD), herpangina and aseptic meningitis. Information on epidemiology profiles NPEV Ural Federal District Western Siberia is very limited, with no published data available. The aim this study to describe incidence among patients different forms enterovirus infections (NPEVIs) during 2022, stratified by age clinical manifestations. A total 265 samples that tested positive...

Objective: Describe susceptibility and molecular profiles among Enterobacteriaceae pathogens to explore if war, other factors, can affect antimicrobial resistance. Methods: Clinical isolates from the Study for Monitoring Antimicrobial Resistance Trends associated with urinary tract intra-abdominal infections between 2011 2015 were identified in Lebanon Jordan. Susceptibility testing characterization performed as per standard methods. Results: A total of 1486 (including unusual pathogens)...

Introduction. Human Noroviruses (HuNoV) are highly contagious pathogens responsible of acute human norovirus infection (HuNoVI). GII.4 is the prevailing genotype in incidence gastroenteritis worldwide. Currently there no studies on molecular monitoring and phylogenetic analysis HuNoV territory Sverdlovsk region, therefore it not possible to objectively assess their genetic diversity.
 The aim study carry out genotyping HNoVI municipalities region: Ekaterinburg, Nizhny Tagil,...

Introduction. Noroviruses are a common cause of acute gastroenteritis with significant public health burden, including outbreaks in facilities, closed and semi-closed settings. This study aims to present global overview trends noroviral epidemiology highlights the important biological properties norovirus. Materials methods. The bibliographic databases (PubMed Russian Science Citation Index) were searched based on keyword “norovirus” (in English languages respectively) without restrictions...

Introduction . The incidence of enterovirus meningitis in Ekaterinburg has long exceeded the average Russia. As a result sanitary, anti-epidemic and restrictive measures related to COVID-19 pandemic, there was decrease morbidity, which led reduction circulation non-polio enteroviruses created risks after lifting restrictions. purpose study is conduct comparative analysis isolated from patients with before pandemic. Materials methods Genotyping positive samples by sequencing VP1 gene...

Introduction . Infant mortality is a universally recognized indicator for social well-being of the population. There need an in-depth analysis causes and factors leading to death infants at home, including determining etiology infectious diseases potentially causing death. The purpose study — determine implication herpesviruses in infant out-of-hospital Materials methods Samples from autopsy material 23 (blood, small intestine, heart, lungs, liver, brain), who died home various causes, were...

АНАЛИТИЧЕСКИЕ ОБЗОРЫ Фундаментальные основы современных подходов к оценке микробиоты кишечника детейМикробиоту у детей рассматривают как один из жизненно важных экстракорпоральных органов, определяющих здоровье человека в детском и взрослом возрасте.В течение первых лет жизни состав кишечной претерпевает значительные изменения, на которые влияет множество факторов.Изменения составе взаимосвязаны с развитием заболеваний желудочно-кишечного тракта разнообразной инфекционной неинфекционной...

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Proficiency testing (PT) is one of the most valuable and important activities for Clinical Microbiology Laboratories (CML) to enroll in ensure accuracy reliability results. This first time conducted nationwide study was warranted assess PT performance activity among CML Lebanon.Four training were organized 110 laboratories involved providing clinical microbiology services. In each activity, five different bacterial species distributed laboratory provide identification (ID) antimicrobial...