A5009248966- Gut microbiota and health
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Clostridium difficile and Clostridium perfringens research
- Infant Nutrition and Health
- Inflammatory Bowel Disease
- Gastrointestinal motility and disorders
- Cancer Research and Treatments
- Microscopic Colitis
- Child Nutrition and Water Access
- Diet and metabolism studies
- Microbial Metabolites in Food Biotechnology
- HIV-related health complications and treatments
- Enterobacteriaceae and Cronobacter Research
- Pediatric health and respiratory diseases
- Microbial Community Ecology and Physiology
- Microbial Metabolism and Applications
- Immune Cell Function and Interaction
- Bacterial Genetics and Biotechnology
- Milk Quality and Mastitis in Dairy Cows
- Diverticular Disease and Complications
- Veterinary medicine and infectious diseases
- Autoimmune and Inflammatory Disorders
- Protein Hydrolysis and Bioactive Peptides
- Biopolymer Synthesis and Applications
Conservatoire National des Arts et Métiers
2010-2024
Délégation Paris 5
2012-2019
Université Paris Cité
2012-2019
Sorbonne Paris Cité
2016-2017
Ecologie Microbienne Lyon
2015
Institut National de la Recherche Agronomique
1995-2004
Virologie et Immunologie Moléculaires
2002
Université de Caen Normandie
1998-1999
Laboratoire de Chimie Physique et Microbiologie pour l'Environnement
1994-1996
Université de Lorraine
1996
In the last few years many attempts have been made to differentiate more than 20 Bifidobacterium species. It has recognized that identification of bifidobacterial species is problematic because phenetic and genetic heterogeneities. order contribute our understanding taxonomy, we studied phylogeny by performing both 16S rRNA 23S (16S-23S) internally transcribed spacer (ITS) sequence analyses. this study, determined sequences five strains representing four species, compared them with available...
Alterations in gut microbiota composition and diversity were suggested to play a role the development of obesity, chronic subclinical inflammatory condition. We here evaluated impact oral consumption monostrain or multi-strain probiotic preparation high-fat diet-induced obese mice. observed strain-specific effect reported dissociation between capacity probiotics dampen adipose tissue inflammation limit body weight gain. A mixture was able improve adiposity, insulin resistance dyslipidemia...
Intestinal microbial community is involved in the pathogenesis of Crohn's disease, but knowledge its potential abnormalities has been limited by impossibility to grow many dominant intestinal bacteria. Using sequence analysis randomly cloned bacterial 16S ribosomal DNA, faecal species from four disease patients and controls were compared. Whereas marked inter-individual differences observed microflora patients, three remained distantly related on basis their operational taxonomic unit...
ABSTRACT The genetic diversity of lactococci isolated from raw milk in the Camembert cheese Registered Designation Origin area was studied. Two seasonal samples (winter and summer) were obtained six farms two areas (Bessin Bocage Falaisien) Normandy. All strains analyzed had a Lactococcus lactis subsp. phenotype, whereas randomly amplified polymorphic DNA (RAPD) technique genotypically identified as members L. or cremoris . genotypes confirmed by performing standard PCR with primers...
The gut microbiota is now recognized as a key parameter affecting the host’s anti-cancer immunosurveillance and ability to respond immunotherapy. Therefore, optimal modulation for preventive therapeutic purposes very appealing. Diet one of most potent modulators microbiota, thus nutritional intervention could be exploited improve host immunity. Here, we show that an inulin-enriched diet, prebiotic known promote immunostimulatory bacteria, triggers enhanced Th1-polarized CD4 + CD8 αβ T...
For infants, the introduction of food other than breast milk is a high risk period due to diarrheal diseases, and may be corroborated with shift in faecal microbiota. This longitudinal study was first undertaken understand effect supplementation on infant's microbiota particularly bifidobacteria. Eleven infants were enrolled. Their analysed using temporal temperature gradient gel electrophoresis (TTGE) bacterial bifidobacterial primers. In parallel, counts followed competitive PCR. Three...
To assess whether juvenile chronic ferric iron ingestion limit colitis and dysbiosis at adulthood in rats mice.Two sets of experiments were designed. In the first set, recently weaned mice either orally administered ferrous (Fe²⁺) salt or (Fe³⁺) microencapsulated for 6 wk. The last week trinitrobenzene sulfonic acid (TNBS) was induced. second received wk also submitted to TNBS during experiments. both experiments, animals sacrificed 7 d after instillation. Severity inflammation assessed by...
The mucosa-associated bacteria (MAB) are suspected of being involved in the pathogenesis Crohn's disease. We analyzed and compared MAB noninflamed inflamed ileal mucosa disease patients (n = 22).Tissue samples from adjacent were taken surgical resection specimens. investigated using fluorescence situ hybridization with 7 group-specific probes temporal temperature gradient gel electrophoresis (TTGE).Samples both obtained 15 patients. distribution bacterial populations was not different...
The establishment and development of the intestinal microbiota is known to be associated with profound short- long-term effects on health full-term infants (FTI), but studies are just starting for preterm (PTI). data also mostly come from western countries little information available Middle East. Here, we determined composition dynamics during first month life PTI (n = 66) FTI 17) in Lebanon. Fecal samples were collected weekly analyzed by quantitative PCR (q-PCR) temporal temperature...
In this study, a competitive PCR was developed to estimate the quantity of bifidobacteria in human faecal samples using two 16S rRNA gene Bifidobacterium genus-specific primers, Bif164f and Bif662r. A PCR-temporal temperature gradient gel electrophoresis (TTGE) with same primers also allowed us describe species present these samples. The product obtained from competitor had 467 bp, 47 bp shorter than products strains. number bifidobacterial cells linear 10 10(8) per assay. Taking into...
This study aimed at evaluating the alteration of colonic microbiota and changes in mucus layer thickness induced by oral administration living bifidobacteria rats. The was performed on rats fed with Bifidobacterium pseudolongum strain Patronus (1010 bacteria per day for 7 days). bacterial led to a large increase (57%, P < 0.05). Both quantitative PCR high-throughput sequencing 16S rRNA gene revealed significant amount genus Patronus, associated decrease Akkermansia muciniphila. could be due...
Total DNA from 21 collection or industrial Bifidobacterium strains was cleaved with various restriction endonucleases. Following electrophoresis, the fragments were subjected to Southern blot hybridization a heterologous [alpha-32P]dCTP-labeled rDNA (genes coding for rRNA) 23S gene probe. The ribosomal patterns allowed all tested be differentiated and previous classifications confirmed. same method used characterize 121 isolates collected intestinal flora of five human volunteers after...
The objective of this study was to assess the possible modifications due amoxicillin-clavulanic acid (AMC) treatment on total bacteria and Bifidobacterium species balance in human colonic microbiota. Eighteen healthy volunteers (19 36 years old) were given a 875/125 mg dose AMC twice day for 5 days. Fecal samples obtained before after antibiotic exposure. After DNA extraction, bifidobacteria specifically quantified using real-time PCR. Dominant monitored over time bacterial bifidobacterial...
Many epidemiological and experimental studies have suggested an important role for dietary fibre (DF) of cereals in the prevention colon cancer. The objective present study was to explain effects DF barley Rihane (BR) on azoxymethane (AOM)-induced aberrant crypt foci (ACF) colonic bacterial diversity rats. Following acclimatisation period, rats were divided into four groups fed a control (C) diet or containing 30 % BR. content twice that C (total 8·69 15·24 BR diet). At 7 8 weeks age,...
This Lebanese study tested the hypothesis that differences would exist in gut microbiota of preterm infants with and without necrotising enterocolitis (NEC), as reported Western countries.This compared 11 NEC controls, all born at 27-35 weeks, three neonatal intensive care units between January 2013 March 2015. Faecal samples were collected key time points, was analysed by culture, quantitative PCR (qPCR) temperature temporal gel electrophoresis (TTGE).The cultures revealed poorly colonised...
Thirty-eight Lactococcus strains, isolated from raw milk produced in two dairy areas Normandy, were identified at the phenotypic level. Only lactis strains with phenotype found samples. Most fermented lactose (97%) and showed proteinase activity (76%). Isolates characterized by RAPD technique rRNA gene restriction analysis. More L. genotype first area, while cremoris predominated second area. was more efficient than analysis differentiating between subsp. genotype. For genotype, method gave...
Randomly amplified polymorphic DNA (RAPD) has been used for the rapid typing of Lactococcus lactis strains isolated from raw milk Camembert region Normandy. It is thought that diversity and perhaps area strain specificity due to climatic geographical factors such wild‐type lactococcal could contribute flavour differences specific features detected same product in different areas. The patterns 58 isolates were analysed by UPGMA dendrograms. At a similarity level 50%, four RAPD clusters...