A5009130930- Organ Transplantation Techniques and Outcomes
- Liver physiology and pathology
- Transplantation: Methods and Outcomes
- Liver Disease and Transplantation
- Organ Donation and Transplantation
- Pancreatic function and diabetes
- Pelvic floor disorders treatments
- Pluripotent Stem Cells Research
- Stress Responses and Cortisol
- Renal Transplantation Outcomes and Treatments
- Simulation-Based Education in Healthcare
- SARS-CoV-2 detection and testing
- Medical Coding and Health Information
- SARS-CoV-2 and COVID-19 Research
- Platelet Disorders and Treatments
- COVID-19 Clinical Research Studies
- Mechanical Circulatory Support Devices
- Body Contouring and Surgery
- Cardiac Structural Anomalies and Repair
- Anorectal Disease Treatments and Outcomes
- Maternal and Perinatal Health Interventions
- Surgical Simulation and Training
- Cardiac, Anesthesia and Surgical Outcomes
- Liver Disease Diagnosis and Treatment
- Heparin-Induced Thrombocytopenia and Thrombosis
MRC Centre for Regenerative Medicine
2007-2025
University of Edinburgh
2007-2025
Edinburgh Royal Infirmary
1998-2025
NHS Blood and Transplant
2020-2025
Pancreas Centre (Canada)
2025
NHS Lothian
2024
National Health Service
2022-2024
Centre for Mental Health
2020
Buckinghamshire Healthcare NHS Trust
2018
Medical Research Council
2008-2010
Human embryonic stem cells (hESCs) are a valuable source of pluripotential primary cells. To date, however, their homogeneous cellular differentiation to specific cell types in vitro has proven difficult. Wnt signaling been shown play important roles coordinating development, and we demonstrate that Wnt3a is differentially expressed at critical stages human liver development vivo. The essential role hepatocyte from hESCs paralleled by our model, demonstrating the importance physiologic...
Organs recovered from donors after circulatory death (DCD) suffer warm ischemia before cold storage which may prejudice graft survival and result in a greater risk of complications transplant. A period normothermic regional perfusion (NRP) the donor reverse these effects improve organ function. Twenty-one NRP retrievals Maastricht category III DCD were performed at three UK centers. was established postasystole via aortic caval cannulation maintained for 2 h. Blood gases biochemistry...
Livers from controlled donation after circulatory death (DCD) donors suffer a higher incidence of nonfunction, poor function, and ischemic cholangiopathy. In situ normothermic regional perfusion (NRP) restores blood supply to the abdominal organs using an extracorporeal circulation for limited period before organ recovery. We undertook retrospective analysis evaluate whether NRP was associated with improved outcomes livers DCD donors. performed on 70 whom 43 were transplanted. These compared...
Patients waitlisted for and recipients of solid organ transplants (SOT) are perceived to have a higher risk contracting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) death; however, definitive epidemiological evidence is lacking. In comprehensive national cohort study enabled by linkage the UK transplant registry Public Health England NHS Digital Tracing services, we examined incidence laboratory-confirmed SARS-CoV-2 infection subsequent mortality in patients on active waiting...
Background . We evaluated whether the use of normothermic regional perfusion (NRP) was associated with increased organ recovery and improved transplant outcomes from controlled donation after circulatory death (cDCD). Methods This is a retrospective analysis UK adult cDCD donors‚ where at least 1 abdominal accepted for transplantation between January 1, 2011, December 31, 2019. Results A mean 3.3 organs transplanted when NRP used compared 2.6 per donor not used. When adjusting organ-specific...
Hepatic progenitor cells play a major role in regenerating diseased liver. In rodents, progenitors forming hepatocytes or cholangiocytes are identified by the stem cell marker Thy-1. The aim of this study was to ascertain whether expressing Thy-1 could be human fetal Midtrimester liver immunostained for Thy-1, cytokeratins 18 and 19, vimentin, CD34, CD45, fibrinogen. Thy-1+ Thy-1+CD34+ populations were purified using fluorescence-activated sorting (FACS). Immunofluorescence mRNA expression...
Purpose of review Published work evaluating machine perfusion DCD (donation after circulatory death) liver grafts in situ and ex is rapidly evolving, with several landmark studies published the last 6 months. The central question transplant remains; which strategies most effectively reduce cholangiopathy? This condition, results repeated hospital admissions, interventions, re-transplantation death, a major deterrent to utilization. considers current evidence mitigation cholangiopathy by...
Abstract Background Deceased Cardiac Donors (DCD) organs are increasingly being used for simultaneous pancreas and kidney (SPK) transplant. The number recovered following in-situ normothermic regional perfusion (NRP) is also increasing. This study reviewed a 10-year UK experience of SPK transplantation NRP in DCD. Methods Data were collected on all first DCD transplants (n=426) performed during 2013-2023 from the Transplant Registry. non-NRP donors compared using adjusted regression models....
Tissue-derived stem cells may offer future liver disease therapies. The developing human provides an excellent model to examine normal hepatic progenitor cell maturation, but candidate populations are poorly characterized. We sought identify putative phenotypes in first-trimester liver, by characterizing the architectural relationship between epithelial, mesenchymal, and hematopoietic lineages. Bipotential hepatoblasts were identified co-expression of hepatocytic (cytokeratin 18, albumin)...
Fluorescence two-dimensional differential gel electrophoresis (2-D DIGE * Difference Gel Electrophoresis (DIGE) technology is covered by US patent number 6043025 and foreign equivalents patents pending. ©Amersham Pharmacia Biotech UK Limited, 2000 – All rights reserved. Amersham Place, Little Chalfont, Buckinghamshire, England, U.K. HP7 9NA. AB, SE-751 84 Uppsala Sweden. Inc, 800 Centennial Avenue, PO Box 1327, Piscataway NJ 08855, USA. Europe GmbH , Munzinger Strasse 9, D-791 11 Freiburg....
Normothermic Regional Perfusion (NRP) has shown encouraging clinical results. However, translation from an experimental to routine procedure poses several challenges. Herein we describe a model that led the implementation of NRP into standard practice in our centre following iterative process refinement incorporating training, staffing and operative techniques. Using this approach achieved four-fold increase trained surgical staff 6-fold competent senior organ preservation practitioners 12...
Pluripotent stem cells are derived from the inner cell mass of preimplantation embryos, and display ability embryonic founder by forming all three germ lineages in vitro. It is well established that cellular niche plays an important role maintenance differentiation. Stem generally have limited function without specialized microenvironment provides key cell-cell contact, soluble mediators, extracellular matrices. We were interested Wnt signaling, particular Wnt3a, played human (hESC)...
Objective To assess the cosmetic appeal of abdominal incisions used for hysterectomy. Design A comparative study. Setting St James's University Hospital, Leeds Participants One hundred women, including 50 consecutive women attending a gynaecology clinic first time and hospital staff. Results Sixty‐eight percent preferred Pfannenstiel incision as choice, while 31% chose laparoscopic assisted vaginal hysterectomy (LAVH). When who had undergone previous surgery were compared with no surgery,...
Background. The agonal phase can vary following treatment withdrawal in donor after circulatory death (DCD). There is little evidence to support when procurement teams should stand down relation time (TTD). We assessed what impact TTD had on outcomes DCD liver transplantation. Methods. Data were extracted from the UK Transplant Registry transplant recipients 2006 2021. was of life-sustaining asystole, and functional warm ischemia systolic blood pressure and/or oxygen saturation falling below...