A5049987934- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Ovarian function and disorders
- Epigenetics and DNA Methylation
- Assisted Reproductive Technology and Twin Pregnancy
- Renal and related cancers
- Seed Germination and Physiology
- Reproductive Physiology in Livestock
- Reproductive Health and Technologies
- Birth, Development, and Health
- Reproductive System and Pregnancy
- Microtubule and mitosis dynamics
- Pluripotent Stem Cells Research
- Genetics, Aging, and Longevity in Model Organisms
- Prenatal Screening and Diagnostics
- Endometriosis Research and Treatment
- Tissue Engineering and Regenerative Medicine
- Animal Genetics and Reproduction
- Pancreatic function and diabetes
- Selenium in Biological Systems
- Adipose Tissue and Metabolism
- Estrogen and related hormone effects
- Microbial Inactivation Methods
- Xenotransplantation and immune response
- Cancer Risks and Factors
China Agricultural University
2015-2024
Xinjiang Academy of Agricultural and Reclamation Science
2021-2024
Ministry of Agriculture and Rural Affairs
2022-2023
Imperial College London
2010-2013
Hammersmith Hospital
2013
Beihang University
2011
Ford Motor Company (United States)
2000
Plastics have caused serious environmental pollution. In recent years, microplastics (MPs) widespread concern about their potential toxicity on animals and humans, especially organ tissue deposition. However, there is little known the reproductive toxic effects of MPs in female mammals. this study, polystyrene (PS-MPs) mice was evaluated after continued exposure for 35 days. Results showed that PS-MPs could accumulate heart, liver, spleen, lung, kidney, brain, large intestine, small uterus,...
In vitro maturation (IVM) and vitrification have been widely used to prepare oocytes before fertilization; however, potential effects of these procedures, such as expression profile changes, are poorly understood. this study, mouse were divided into four groups subjected combinations in and/or treatments. RNA-seq silico pathway analysis identify differentially expressed genes (DEGs) that may be involved oocyte viability after vitrification. Our results showed 1) 69 IVM, 66 which...
Oocyte activation deficiency (OAD) is a primary cause of fertilization failure following intracytoplasmic sperm injection (ICSI), problem that can potentially be overcome through artificial oocyte (AOA). However, concerns persist regarding the safety and efficacy AOA techniques in clinical practice. In this study, nanosecond pulsed electric field (nsPEF) proposed as safe controllable method for vitro by precisely manipulating Ca2+ signaling ooplasm. Mouse oocytes collected from oviducts were...
This study was conducted to investigate the pattern of DNA methylation in vitrified-thawed mouse oocytes and their vitro fertilized early embryos. Firstly, at metaphase II (MII) stage meiosis were allocated randomly into three groups: (1) untreated (control); (2) exposed vitrification solution without being plunged liquid nitrogen (toxicity); or (3) vitrified by open-pulled straw (OPS) method (vitrification). Oocytes from all groups subsequently vitro. The level MII embryos then examined...
Overweight or obese women seeking pregnancy is becoming increasingly common. Human maternal obesity gives rise to detrimental effects during reproduction. Emerging evidence has shown that these abnormities are likely attributed oocyte quality. Oxidative stress induces poor conditions, but whether mitochondrial calcium homeostasis plays a key role in status remains unresolved. Here, we established Ca 2+ overload model mouse oocytes. Knockdown gatekeepers of the uniporters Micu1 and Micu2 as...
Maternal aging can impair the quality and decrease developmental competence of ovulated oocytes. In this study, compromised germinal vesicle breakdown (GVBD) was found in aged mice Furthermore, we observed increased reactive oxygen species (ROS) mitochondrial Ca2+ levels, along with reduced temperature also changed crotonylation level Forkhead box O3 (FoxO3a), a member forkhead protein family involved regulation cell survival life span reached peak metaphase II stage. Compared younger group,...
Abstract Background Irreversible cryodamage caused by oocyte vitrification limited its wild application in female fertility preservation. Antioxidants were always used to antagonist the oxidative stress vitrification. However, comprehensive mechanism underlying protective role of antioxidants has not been studied. Procyanidin B2 (PCB2) is a potent natural antioxidant and functions response are still unknown. In this study, effects PCB2 on vitrified-thawed oocytes subsequent embryo...
Metabolic balance is essential for oocyte maturation and acquisition of developmental capacity. Suboptimal conditions in vitro cultures would lead to lipid accumulation finally result disrupted metabolism. However, the effect mechanism underlying catabolism development remain elusive currently. In present study, we observed enhanced capacity Procyanidin B2 (PCB2) treated oocytes during maturation. Meanwhile, reduced oxidative stress declined apoptosis were found after PCB2 treatment. Further...
Plasma membrane damage in vitrified oocytes is closely linked to mitochondrial dysfunction. However, the mechanism underlying mitochondria-regulated stability not elucidated. A growing body of evidence indicates that activity plays a pivotal role cell adaptation. Since mitochondria work at higher temperature than constant external cell, we hypothesize suppressing would protect from extreme stimuli during vitrification. Here show metformin suppresses by reducing temperature. In addition,...