Chitosan acquires bacteriostatic properties via protonation of its amino groups. However, much the literature assumes that chitosan itself inhibits growth bacteria. This article presents a comparative study nonwovens modified with various acids, including acetic, propionic, butyric, and valeric organic as well hydrochloric acid. The aim was to determine which acid salts influence antibacterial antifungal activity chitosan-based materials. Two methods were used modify (formation ammonium...

Abstract A collection of N ‐triazinylammonium sulfonates, designed according to the concept “superactive esters”, was obtained by treatment ammonium sulfonates with 2‐chloro‐4,6‐dimethoxy‐1,3,5‐triazine. The structure tertiary amine as well sulfonate anion influenced their reactivity and stability in , ‐dimethylformamide (DMF) solution. reagents were successfully used solution‐ solid‐phase synthesis Z‐, Boc‐, Fmoc‐protected peptides containing natural unnatural sterically hindered amino...

Algae are employed commonly in cosmetics, food and pharmaceuticals, as well feed production biorefinery processes. In this study, post-fermentation leachate from a biogas plant which exploits stillage maize silage was utilized culture medium for Chlorella vulgaris. The content of polyphenols hydrophilic extracts the vulgaris biomass determined, were evaluated their antioxidant activity (DPPH assay), antibacterial (against Escherichia coli, Lactobacillus plantarum, Staphylococcus aureus,...

Carbon-based nanomaterials (CBN) are currently used in many biomedical applications. The research includes optimization of single grain size and conglomerates pure detonated nanodiamond (DND), modified particles graphene oxide (GO) order to compare their bactericidal activity against food pathogens. Measurement zeta potential was performed using the Dynamic Light Scattering (DLS) method. Surface morphology evaluated a Scanning Electron Microscope (SEM) confocal microscope. X-ray diffraction...

Two new rigid bi-aromatic linkers for synthesis of peptide arrays by SPOT methodology were obtained from cellulose treated with 2,4-dichloro-6-methoxy-1,3,5-triazine. Reaction m-phenylenediamine gave non-cleavable TYPE I linker which enabled attachment the peptides via resistant to harsh reaction conditions amide, ether, and amine bonds. 3-Fmoc-aminobenzoic acid followed thermal isomerization intermediate "superactive" ester producing an amide-like bond II that was very stable during...

A series of new analogs nitrogen mustards (4a–4h) containing the 1,3,5-triazine ring substituted with dipeptide residue were synthesized and evaluated for inhibition both acetylcholinesterase (AChE) β-secretase (BACE1) enzymes. The AChE inhibitory activity studies carried out using Ellman’s colorimetric method, BACE1 fluorescence resonance energy transfer (FRET). All compounds displayed considerable inhibition. most active against enzymes 4a, an concentration IC50 = 0.051 µM; 0.055 µM 9.00...

Bone tissue defects resulting from periodontal disease are often treated using guided regeneration (GTR). The barrier membranes utilized here should prevent soft infiltration into the bony defect and simultaneously support bone regeneration. In this study, we designed a degradable poly(l-lactide-co-glycolide) (PLGA) membrane that was surface-modified with cell adhesive arginine-glycine-aspartic acid (RGD) motifs. For novel method of manufacture, RGD motifs were coupled non-ionic amphiphilic...

Current restrictions on the use of antibiotics, associated with increases in bacterial resistance, require new solutions, including materials antibacterial properties. In this study, copper alginate fibers obtained using classic wet method were used to make nonwovens which modified arginine-glycine-aspartic acid (RGD) derivatives. Stable polysaccharide-peptide conjugates formed by coupling 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium toluene-4-sulfonate (DMT/NMM/TosO−), and...

Aim: Anchoring folic acid (FA) with a biomimetic peptidic linker resistant to proteolytic degradation act as homing device on functionalized carbon nanotubes. Materials & methods: Ethylenediamine was attached oxidized multiwalled nanotubes (MWNTs) using 4-(4,6-dimethoxy-[1,3,5]triazin-2-yl)-4-methylmorpholinium tetrafluoroborate. FA coupled 6-aminohexanoic and derivatives of β-alanine, affording four intermediates, which connected the MWNTs via linkers various lengths. Results: Biomimetic...

Abstract Despite the development of pharmacy, there are still incurable diseases for which medicine has not been found yet. Because many asymptomatic in their first stage development, often early detection is crucial factor combating them. The article describes process synthesis carbon nanotubes (CNTs) can be useful medical diagnostics. CNTs were synthesized by chemical vapor deposition. obtained material was subjected to functionalization – attaching fluorescent markers. In order check...

In this study, three independent methods were used to identify short fragment of both chains human insulin which are prone for aggregation. addition, circular dichroism (CD) research was conducted understand the progress aggregation over time. The fragments (deca- and pepta-peptides) obtained by solid-phase synthesis using 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium toluene-4-sulfonate (DMT/NMM/TosO-) as a coupling reagent. Systematic studies allowed identification new...

To identify the shortest components of A13-A19, B12-B17 fragments capable for fibrillation and to validate dependability aggregation on presence hydroxyl group engaged in 'tyrosine kissing'.Fragments A13-A19 insulin all shortened analogues were obtained by using DMT/NMM/TosO(-) as a coupling reagent. The was studied three independent tests.Studies susceptibility truncated analogs amyloidogenic core show groups peptides.Truncation A13-A419 fragment shows that fibrous structures are formed...

In this study, N-methylated analogs of hot-spots insulin were designed and synthesized, in the expectation that they would inhibit aggregation both entire hormone. Synthesis "amyloidogenic" containing amino acid residues was performed by microwave-assisted solid phase according to Fmoc/tert-Bu strategy. As a coupling reagent 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium toluene-4-sulfonate (DMT/NMM/TosO-) used. Three independent methods applied studies complexes with its...

The cross-linking of polysaccharides is a universal approach to affect their structure and physical properties. Both chemical methods are used for this purpose. Although provides good thermal mechanical stability the final products, compounds as stabilizers can integrity cross-linked substances or have toxic properties that limit applicability products. These risks might be mitigated by using physically gels. In present study, we attempted obtain hybrid materials based on carbon nonwovens...

A laser system with a wavelength of 1064 nm was used to generate sp² carbon on the surfaces nanodiamond particles (NDPs). The modified by microplasma NDPs were analysed using FT-IR and Raman spectroscopy. spectra confirmed that graphitization had occurred NDPs. extent depended average power in treatment process. analysis revealed presence C=C bonds all laser-modified powder. characteristic peaks for olefinic much more intense than case untreated powder grew intensity as increased. olefinized...

Summary This study provides new information on the cellular effects of 1,3,5-triazine nitrogen mustards with different peptide groups in DLD and Ht-29 human colon cancer cell lines. A novel series 2,4,6-trisubstituted derivatives bearing 2-chloroethyl oligopeptide moieties was designed synthesized . The most cytotoxic derivative triazine an Ala-Ala-OMe substituent ring (compound 7b ). compound induced time- dose-dependent cytotoxicity DLD-1 HT-29 furthermore apoptosis through intracellular...

Dual action alkyl(aryl)amino-1,3,5-triazines functionalized with nitrogen mustards were obtained by treating 2-alkyl(aryl) amino-4-chloro-6-methoxy-1,3,5-triazines amines or amino acid methyl esters, followed reactions 1,4-diazabicyclo[2.2.2]octane (DABCO) and rearrangement an opening diazabicyclic fragment, leading to the formation of 2-chloroethylamino moiety. In vitro antitumor activity was tested in standard human breast cancer MCF-7 MDA-MB-231cell lines using flow cytometry, based on...

The pharmacophore properties of a new series potential purinoreceptor (P2X) inhibitors determined using coupled neural network and the partial least squares method with iterative variable elimination (IVE-PLS) are presented in ligand-based comparative study molecular surface by analysis (CoMSA). Moreover, we focused on interpretation noticeable variations selectiveness interactions individual inhibitor-receptors due to their physicochemical properties; therefore, library artificial dipeptide...

4‐(4,6‐Dimethoxy‐1,3,5‐triazin‐2‐yl)‐4‐methylmorpholinium toluene‐4‐sulfonate ( DMT / NMM /TsO − ), a representative member of the inexpensive and environmentally‐friendly N ‐triazinylammonium family sulfonates, has been found to be very effective coupling reagent for synthesis amides, esters peptides in solution. This study confirms usefulness peptide solution, starting from Z‐, Fmoc‐, Boc‐protected substrates as well unnatural building blocks. Peptide with produced high yields, crude...

This study investigates the propensity of short peptides to self-organize and influence aggregates on cell cultures. The dipeptides were derived from both enantiomers identical aromatic amino acids tripeptides prepared two with one cysteine or methionine residue in C-terminal, N-terminal, central position. formation absence fibrous structures under physiological conditions was established using Congo Red Thioflavine T assays as well by microscopic examination normal polarized light. vitro...

Summary 1,3,5-triazine is an important heterocyclic skeleton for mono, two or three 2-chloroethylamine groups. The study presented here provides novel information on cellular effects of with groups in glioblastoma LBC3, LN-18 and LN-229 cell lines. In our study, the most cytotoxic effect was observed ( 12f compound). It has been demonstrated that induce time- dose-dependent cytotoxicity all investigated glioma Apart from cells, treated compound, we noticed strong induction apoptosis....

New materials that are as similar possible in terms of structure and biology to the extracellular matrix (external environment) cells great interest for regenerative medicine. Oligoproline oligohydroxyproline derivatives (peptides 2-5) potential mimetics collagen fragments. Peptides 2-5 have been shown be model fragment (H-Gly-Hyp-Pro-Ala-Hyp-Pro-OH, 1) both their spatial biological activity. In this study, peptides were covalently bound nonwovens based on chitosan calcium alginate....