A5029216876- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cancer Genomics and Diagnostics
- DNA Repair Mechanisms
- CAR-T cell therapy research
- vaccines and immunoinformatics approaches
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Renal Diseases and Glomerulopathies
- Virus-based gene therapy research
- Multiple and Secondary Primary Cancers
- RNA modifications and cancer
- Polyomavirus and related diseases
- Cancer-related Molecular Pathways
- Science, Research, and Medicine
- Neuroblastoma Research and Treatments
- Single-cell and spatial transcriptomics
- Protein Degradation and Inhibitors
- Lymphoma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
- Peptidase Inhibition and Analysis
Moffitt Cancer Center
2016-2025
University of South Florida
2016-2025
Florida College
1996-2018
University of Tampa
2013-2015
Institute of Molecular Medicine
2013
Harvard University
1985-1990
Columbia University
1983-1988
Koester Performance Research (United States)
1985
Albert Einstein College of Medicine
1985
A 435-kilobase (kb) DNA segment, which is centromeric to HLA-B in the human major histocompatibility complex, was isolated by chromosome walking with overlapping cosmids. Within cloned region, genes for tumor necrosis factors (TNFs) alpha and beta were 210 kb apart. The homolog of a mouse gene, B144, located next TNF alpha. Moreover, presence additional suggested large cluster CpG islands. With cosmid probes, several distinct transcripts detected RNA samples from variety cell lines....
Tumor immunoscoring is rapidly becoming a universal parameter of prognosis, and T-cells isolated from tumor masses are used for ex vivo amplification readministration to patients facilitate an antitumor immune response. We recently exploited the cancer genome atlas (TCGA) RNASeq data assess T-cell receptor (TcR) expression and, in particular, discovered strong correlations between major histocompatibility class II (MHCII) TcR-α constant region levels. In this article, we describe results...
It has recently become apparent that it is possible to characterize productively recombined, T-cell receptor (TcR) gene segments in tumor exome files, which presumably include representations of the DNA other cells microenvironment. Similar characterizations have been done for TcR recombinations specimen RNASeq files. While files used immunoglobulin segment tumors closely related B-cells, yet be characterized putative microenvironment solid tumors. Here we report a novel scripted algorithm...
Abstract It became apparent several years ago that RNAseq and exome files prepared from tissue could be mined for adaptive immune receptor (IR) recombinations, which has given extra value to datasets originally intended gene expression or mutation studies. For example, recovery of IR recombination reads tumour specimen genomics can correlate with survival rates. In particular, many benchmarking processes have been applied the two sets obtained cancer genome atlas files, but these never...
Pancreatic cancer (PC) is a deadly disease with grim prognosis. tumor derived factors (TDF) contribute to the induction of an immunosuppressive microenvironment (TME) that impedes effectiveness immunotherapy. PC-induced microRNA-155 (miRNA-155) represses expression Src homology 2 (SH2) domain-containing Inositol 5′-phosphatase-1 (SHIP-1), regulator myeloid cell development and function, thus impacting anti-tumor immunity. We recently reported bioflavonoid apigenin (API) increased SHIP-1...
Given the ongoing challenges regarding specific roles of viral infections in cancer etiology, or as co-morbidities, this study assessed potential associations between anti-viral, T-cell receptor (TCR) complementarity domain region-3 (CDR3s), and clinical outcomes for ovarian cancer. TCR CDR3s were isolated from specimens a determination which patients had anti-viral whether those better worse outcomes. Analyses revealed that with exact matches anti-Epstein-Barr virus (EBV) CDR3 amino acid...
241 Background: In prostate cancer, identifying biomarkers to predict patients who are at higher risk for metastatic disease remains a key question. The interaction of oncogenes such as MYC with the adaptive immune system may be potential marker worse progression; example, -amplified neuroblastoma has been shown immunologically cold both in vitro and respect amount receptor (IR) recombinations recovered from genomics files. Thus, we performed correlative study relationship between...
While the Warburg effect is well-known and frequently studied, molecular features that facilitate increased tumor cell glycolytic activity have yet to be extensively investigated. We hypothesized amplification of genes encoding proteins related glucose metabolism could a mechanism glycolysis. Thus, we applied precision-guided copy number variation analysis approach GLP1R, AMFR, GCG, GPI, ACTA1 across three different cancer types. Results indicated higher CNs GLP1R in glioblastoma were...
Abstract Introduction: While the role of anti-cytomegalovirus (CMV) T-cell responses in cancer setting is becoming increasingly recognized, association between CMV and survival outcomes has not been subject to study through more recent immunogenomic approaches. This aimed investigate anti-CMV receptor (TCR) sequences improved two types cancers where remains currently underexplored, stomach adenocarcinoma (STAD) soft tissue sarcoma (SARC). The significance this that, our knowledge, it first...
The Adaptive Immune Receptor Repertoire (AIRR) Community is a research-driven group that establishing clear set of community-accepted data and metadata standards; standards-based reference implementation tools; policies practices for infrastructure to support the deposit, curation, storage, use high-throughput sequencing from B-cell T-cell receptor repertoires (AIRR-seq data). AIRR Data Commons distributed system repositories utilizes common model, query language, interoperability formats...
Abstract Immune-modulating systemic therapies are often used to treat advanced cancer such as metastatic clear cell renal carcinoma (ccRCC). Used alone, sequence-based biomarkers neither accurately capture patient dynamics nor the tumor immune microenvironment. To better understand ecology of this microenvironment, we quantified infiltration across three distinct ccRCC cohorts using complementarity determining region-3 (CDR3) sequence recovery counts in tumor-infiltrating lymphocytes and a...
Despite the fact that only modest adaptive immune system related approaches to treating Alzheimer's disease (AD) are available, an immunogenomics approach study of AD has not yet substantially advanced.Thus, we sought better understand receptor chemical features in setting.We characterized T-cell alpha (TRA) complementarity determining region-3 (CDR3) physicochemical and identified TRA CDR3 homology groups, represented by recombination reads extracted from 2,665 AD-related, blood-...