A5085772853- DNA and Biological Computing
- Modular Robots and Swarm Intelligence
- Distributed systems and fault tolerance
- Gene Regulatory Network Analysis
- Origins and Evolution of Life
- Muscle Physiology and Disorders
- Network Traffic and Congestion Control
- Peer-to-Peer Network Technologies
- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Mobile Agent-Based Network Management
- Cancer-related Molecular Pathways
- Evolutionary Algorithms and Applications
- Advanced Software Engineering Methodologies
- Toxoplasma gondii Research Studies
- Streptococcal Infections and Treatments
- Parvovirus B19 Infection Studies
- Neonatal and Maternal Infections
- Antifungal resistance and susceptibility
- Malaria Research and Control
- Opportunistic and Delay-Tolerant Networks
- Ubiquitin and proteasome pathways
- Parasitic Infections and Diagnostics
- Biotin and Related Studies
- Cellular Automata and Applications
Universidade de São Paulo
2015-2024
Instituto de Medicina Avançada
2018-2023
Instituto de Medicina Tropical
2013-2023
Fundação de Amparo à Pesquisa do Estado de São Paulo
2017
Hospital São Paulo
2017
Université de Strasbourg
2010-2013
Laboratoire des Sciences de l'Ingénieur, de l'Informatique et de l'Imagerie
2010-2013
University of York
2012
University of Basel
2005-2011
Swiss Federal Archives
2008-2010
Toxoplasmosis may be life-threatening in fetuses and immune-deficient patients. Conventional laboratory diagnosis of toxoplasmosis is based on the presence IgM IgG anti-Toxoplasma gondii antibodies; however, molecular techniques have emerged as alternative tools due to their increased sensitivity. The aim this study was compare performance 4 PCR-based methods for toxoplasmosis. One hundred pregnant women who seroconverted during pregnancy were included study. definition cases a 12-month...
Performance variation among PCR systems in detecting Toxoplasma gondii has been extensively reported and associated with target genes, primer composition, amplification parameters, treatment during pregnancy, host genetic susceptibility genotypes of different parasites according to geographical characteristics.A total 467 amniotic fluid samples from T. IgM- IgG-positive Brazilian pregnant women being treated for 1 6 weeks at the time amniocentesis (gestational ages 14 25 weeks).One...
Hand-foot-and-mouth disease (HFMD) is a highly contagious viral commonly associated to Enteroviruses (EV). During 2018, Brazil faced massive HFMD outbreaks spread across the country. This study aimed characterize EV responsible for outbreak that occurred in Paraiba State, Brazilian Northeastern region, followed by phylogenetic analysis detail information on its genetic diversity. A total of 49 serum samples (one from each patient) collected children ≤ 15 years old, clinically diagnosed with...
Abstract Linkage studies have indicated that a gene on chromosome arm 17q, designated BRCAI , confers susceptibility to familial breast and ovarian cancer. To investigate the possible involvement of in sporadic cancer we analysed loss heterozygosity (LOH) panel 100 primary tumours using 10 PCR‐based polymorphic markers from 17q12–21. Allele losses were detected 40 informative for at least one analysed. Of these deleted tumours, 27 showed partial or interstitial 17q. The pattern LOH with...
Bacterial sepsis remains a major cause of mortality and blood cultures are the gold standard laboratory diagnosis even though they lack sensitivity in neonates. Culturenegative sepsis, also known as clinical has long been considered neonatal intensive care units because, well culture-positive infants, culture-negative neonates have worse prognosis comparison with non-infected ones. Quantitative amplifications used to detect bacterial infections but results only qualitative way (positive or...
To correlate neonatal and infant clinical outcome with parasite load in amniotic fluid (AF).We conducted a retrospective cohort study of 122 children whose mothers had toxoplasmosis during pregnancy. The were monitored from birth to 12 months old. Stored AF samples obtained at maternal diagnosis tested by quantitative polymerase chain reaction. Gestational age infection, reaction results, anti-Toxoplasma gondii immunoglobulin (Ig) M, correlated.Maternal infection occurred 18 (14.7%) 104...
Host and parasite genotypes are among the factors associated with congenital toxoplasmosis pathogenesis. As HLA class II molecules play a key role in immune system regulation, aim of this study was to investigate whether HLA-DQA1/B1 alleles susceptibility or protection toxoplasmosis. One hundred twenty-two fetuses 103 without were studied. The two groups comparable according number socio-demographic genetic variables. typed by PCR-SSP. In HLA-DQA1 region, allele frequencies showed that...
The relative expression of estrogen receptor (ER) and progesterone (PR) mRNA transcripts was measured in 71 primary breast-cancer biopsies. ER PR binding activity were estimated parallel by the dextran-coated-charcoal method. There a close correlation between amount estradiol activity. Tumors from post-menopausal patients contained higher levels than those pre-menopausal patients. Northern-blot analysis indicated presence major band 6.3 kb all mRNA-positive tumors. Some tumors showed,...
Bacteria of the genus Bartonella are emerging pathogens detected in lymph node biopsies and aspirates probably caused by increased concentration bacteria. Twenty-three samples 18 patients with clinical, laboratory and/or epidemiological data suggesting bartonellosis were subjected to three nested amplifications targeting a fragment 60-kDa heat shock protein (HSP), internal transcribed spacer 16S-23S rRNA (ITS) cell division (FtsZ) henselae, order improve detection clinical samples. In first...
Thirty-four Candida isolates were analyzed by random amplified polymorphic DNA using the primer OPG-10:24 albicans; 4 tropicalis; 2 parapsilosis; dubliniensis; 1 glabrata and krusei. The UPGMA-Pearson correlation coefficient was used to calculate genetic distance between different groupings. Samples classified as identical (correlation of 100%); highly related samples (90%); moderately (80%) unrelated (< 70%). results showed that RAPD proposed capable classifying coherently (such same...
Epstein-Barr virus (EBV), the causative agent of infectious mononucleosis, plays a significant role as cofactor in process tumorigenesis, and has consistently been associated with variety malignancies especially immunocompromised patients. Forty-four children adolescents (21 liver transplant patients, 7 heart transplant, 5 AIDS, 3 autoimmune hepatitis, 2 nephritic syndromes, medullar aplasia, primary immunodeficiency disorder 1 thrombocytopenic purpura systemic lupus erythematosus)...
The inherently decentralized nature of artificial chemical computing models makes them particularly attractive for building bio-inspired software with self-organizing and emergent properties. Yet it is not straightforward to construct such programs, either manually or automatically. We are exploring the potential programming automatic programming, in context autonomic environments where must operate unsupervised unlimited periods time. enhancing Fraglets language support intrinsic genetic...
Only a small percentage of individuals living in endemic areas develop severe malaria suggesting that host genetic factors may play key role. This study has determined the frequency single nucleotide polymorphisms (SNPs) some pro and anti-inflammatory cytokine gene sequences: IL6 (-174; rs1800795), IL12p40 (+1188; rs3212227), IL4 (+33; rs2070874), IL10 (-3575; rs1800890) TGFb1 (+869; rs1800470), by means PCR-RFLP. Blood samples were collected from 104 symptomatic 37 asymptomatic subjects....
Objective To compare clinical, functional and immunological features between elderly asthmatics with long-standing asthma (LSA) those late-onset (LOA).
In this study we investigated the incidence of mutations and loss heterozygosity (LOH) TP53 gene in DNA samples from paired tumor adjacent normal tissue 90 patients with untreated squamous-cell carcinoma head neck. Evidence for were demonstrated 53% (48/90) cases analyzed. All also examined heterozygosity, using a PCR-based polymorphic marker at TP53. LOH was found 36 out 72 (50%) informative cases. Direct sequencing PCR products performed 45 evidence mutations. The results revealed presence...