A5033292249- Hormonal and reproductive studies
- Hormonal Regulation and Hypertension
- Sexual Differentiation and Disorders
- Estrogen and related hormone effects
- Prostate Cancer Treatment and Research
- Aldose Reductase and Taurine
- Pharmacogenetics and Drug Metabolism
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive biology and impacts on aquatic species
- Reproductive Physiology in Livestock
- Ovarian function and disorders
- Analytical Chemistry and Chromatography
- Steroid Chemistry and Biochemistry
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Zebrafish Biomedical Research Applications
- Physiological and biochemical adaptations
- Adipose Tissue and Metabolism
- Genetic and phenotypic traits in livestock
- Mass Spectrometry Techniques and Applications
- Cancer, Lipids, and Metabolism
- Growth Hormone and Insulin-like Growth Factors
- HIV-related health complications and treatments
- Vitamin D Research Studies
- Metabolomics and Mass Spectrometry Studies
- Seaweed-derived Bioactive Compounds
Stellenbosch University
2015-2024
University of Birmingham
2017-2024
Medical Research Council
2024
University of Sheffield
2018
Abstract Context: Androgen excess is a defining feature of polycystic ovary syndrome (PCOS), but the exact origin hyperandrogenemia remains matter debate. Recent studies have highlighted importance 11-oxygenated C19 steroid pathway to androgen metabolism in humans. In this study, we analyzed contribution androgens women with PCOS. Methods: One hundred fourteen PCOS and 49 healthy control subjects underwent measurement serum by liquid chromatography-tandem mass spectrometry. Twenty-four–hour...
Dihydrotestosterone (DHT) is regarded as the most potent natural androgen and implicated in development progression of castration resistant prostate cancer (CRPC). Under castrate conditions, DHT produced from metabolism adrenal precursors, DHEA androstenedione. Recent studies have shown that steroid 11β-hydroxyandrostenedione (11OHA4) serves precursor to androgens 11-ketotestosterone (11KT) 11-ketodihydrotestosterone (11KDHT). In this study we comprehensively assess androgenic activity 11KT...
Abstract Steroid biosynthesis and metabolism are reflected by the serum steroid metabolome and, in even more detail, 24-hour urine metabolome, which can provide unique insights into alterations of flow output indicative underlying conditions. Mass spectrometry–based profiling has allowed for identification multisteroid signatures associated with disorders that be used personalized approaches to diagnosis, differential prognostic prediction. Additionally, analysis been successfully as a...
Abstract Background The enzyme 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) determines prereceptor metabolism and activation of glucocorticoids within peripheral tissues. Its dysregulation has been implicated in a wide array metabolic diseases, leading to the development selective 11β-HSD1 inhibitors. We examined impact reversible competitive inhibitor, AZD4017, on profile an overweight female cohort with idiopathic intracranial hypertension (IIH). Methods conducted UK multicenter...
Multi-steroid profiling is a powerful analytical tool that simultaneously quantifies steroids from different biosynthetic pathways. Here we present an ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) assay for the of 23 using post-column infusion ammonium fluoride.Following liquid-liquid extraction, were chromatographically separated over 5 min Phenomenex Luna Omega C18 column and water (0.1 % formic acid) methanol gradient. Quantification was performed on...
Abstract Context The clinical effects of classical 3β-hydroxysteroid dehydrogenase 2 (3βHSD2) deficiency are insufficiently defined due to a limited number published cases. Objective To evaluate an integrated steroid metabolome and the short- long-term features 3βHSD2 deficiency. Design Multicenter, cross-sectional study. Setting Nine tertiary pediatric endocrinology clinics across Turkey. Patients Children with diagnosis Main Outcome Measures Clinical manifestations,...
Epitestosterone is a stereoisomer of the active androgen testosterone and its circulating concentrations are similar to those in women children. However, biological function pathways metabolism remain unknown. The structural similarity suggests potential modulation receptor signalling. It well established that conversion 5α-dihydrotestosterone enhances local In this study, we show epitestosterone metabolized 5α-dihydroepitestosterone by both human steroid 5α-reductase isoforms, SRD5A1...
Context:The classic androgen synthesis pathway proceeds via dehydroepiandrosterone, androstenedione, and testosterone to 5α-dihydrotestosterone. However, 5α-dihydrotestosterone can also be achieved by an alternative originating from 17α-hydroxyprogesterone (17OHP), which accumulates in congenital adrenal hyperplasia (CAH). Similarly, recent work has highlighted androstenedione-derived 11-oxygenated 19-carbon steroids as active androgens, CAH, androstenedione is generated directly 17OHP. The...
In the last decades in vitro studies highlighted potential for crosstalk between Hypoxia-Inducible Factor-(HIF) and glucocorticoid-(GC) signalling pathways. However, how this interplay precisely occurs vivo is still debated. Here, we use zebrafish larvae (Danio rerio) to elucidate what degree hypoxic affects endogenous glucocorticoid pathway vice versa, vivo. Firstly, our results demonstrate that presence of upregulated HIF signalling, both receptor (Gr) responsiveness cortisol levels are...
Abstract Background Given the rarity of 11β-hydroxylase deficiency (11βOHD), there is a paucity data about differences in clinical and biochemical characteristics classic (C-11βOHD) nonclassic 11βOHD (NC-11βOHD). Objective To characterize multicenter pediatric cohort with 11βOHD. Method The were retrospectively retrieved. CYP11B1 gene sequencing was performed. Seventeen plasma steroids quantified by liquid chromatography-mass spectrometry compared to that controls. Results 102 patients...