A5069236946- Hormonal and reproductive studies
- Adrenal Hormones and Disorders
- Hormonal Regulation and Hypertension
- Stress Responses and Cortisol
- Sexual Differentiation and Disorders
- Pituitary Gland Disorders and Treatments
- Ovarian function and disorders
- Renal Transplantation Outcomes and Treatments
- Pharmacological Effects and Assays
- Pesticide Residue Analysis and Safety
- Transplantation: Methods and Outcomes
- Adrenal and Paraganglionic Tumors
- Antibiotics Pharmacokinetics and Efficacy
- Mass Spectrometry Techniques and Applications
- Hypothalamic control of reproductive hormones
- Estrogen and related hormone effects
- Metabolism and Genetic Disorders
- Growth Hormone and Insulin-like Growth Factors
- Gastrointestinal motility and disorders
- Salivary Gland Disorders and Functions
- Pharmacological Effects and Toxicity Studies
- Muscle metabolism and nutrition
- Metabolomics and Mass Spectrometry Studies
- Organ Transplantation Techniques and Outcomes
- Pharmacology and Obesity Treatment
University of Manchester
2016-2025
Wythenshawe Hospital
2011-2024
Manchester University NHS Foundation Trust
2015-2024
Manchester Academic Health Science Centre
2015-2024
NIHR Manchester Biomedical Research Centre
2016-2023
Royal Hospital for Children
2021
LMC Diabetes & Endocrinology (Canada)
2003-2016
National Health Service
2006-2016
Manchester University
2004-2016
British School at Rome
2015
Health and lifestyle factors are associated with variations in serum testosterone levels ageing men. However, it remains unclear how age-related changes may be attenuated by modifications. The objective was to investigate the longitudinal relationships between health hormones of reproductive endocrine axis men.A survey 2736 community-dwelling men aged 40-79 years at baseline recruited from eight centres across Europe. Follow-up assessment occurred mean (±S.D.) 4.4±0.3 later.Paired results...
Adrenal aldosterone excess is the most common cause of secondary hypertension and associated with increased cardiovascular morbidity. However, adverse metabolic risk in primary aldosteronism extends beyond hypertension, rates insulin resistance, type 2 diabetes, osteoporosis, which cannot be easily explained by excess.We performed mass spectrometry-based analysis a 24-hour urine steroid metabolome 174 newly diagnosed patients (103 unilateral adenomas, 71 bilateral adrenal hyperplasias)...
Abstract To assess the inherent potential for detecting mild to moderate reductions in glomerular filtration rate, this study determined biological variability of serum cystatin C and creatinine 12 healthy subjects. After accounting analytical variation, interindividual variance accounted 93% intraindividual 7% variation. As such, lie outside assay reference interval, some subjects must exceed 13 SD from their usual mean value, whereas others, a change only 2 would be sufficient. For C,...
Salivary cortisol measurement is used as a practical surrogate for serum free cortisol. However, parotid tissue harbors 11β-hydroxysteroid dehydrogenase (11β-HSD2) activity converting to cortisone.This study was designed assess the impact of 11β-HSD2 on salivary PATIENTS, DESIGN, AND OUTCOME MEASURES: Study participants with changes in circulating corticosteroid-binding globulin (CBG) (±oral contraceptive, functionally CBG null) and controls were studied during adrenal stimulation by ACTH...
Voriconazole is a new antifungal agent effective in the treatment of invasive aspergillosis. Interpatient variation plasma concentrations considerable—more than 100-fold. We describe 3 patients with diverse manifestations toxicity (e.g., hallucinations, hypoglycemia, electrolyte disturbance, and pneumonitis) possibly attributable to high voriconazole concentrations. Measurement could be helpful optimizing dosages.
RIA-based sex hormone measurements offer only limited precision and specificity in the low concentration range of women. Therefore, we aimed to establish age-specific reference ranges for serum concentrations women using mass spectrometry quantile regression. Data from 985 aged 20–80 yr, recruited prospective Study Health Pomerania, were included analyses. Quantile regressions models performed calculate 2.5th 97.5th percentiles Serum total testosterone (TT) androstenedione (AD) measured by...
Abstract Background: The recent interest of clinical laboratories in developing serum testosterone assays based on isotope dilution–liquid chromatography–tandem mass spectrometry (ID-LC-MS/MS) stems from the lack accuracy direct immunoassays. In this study, we assessed and state standardization (traceability) 4 published ID-LC-MS/MS procedures a method comparison with an ID–gas chromatography (GC)–MS reference measurement procedure listed database Joint Committee for Traceability Laboratory...
During aging, total testosterone (TT) declines and SHBG increases, resulting in a greater decrease calculated free T (cFT). Currently, guidelines suggest using TT to diagnose androgen deficiency reserve cFT only for men with borderline TT.Our objective was investigate if either low or is more strongly associated androgen-related clinical endpoints.A of 3334 community-dwelling men, aged 40-79 years, were included this study. Differences variables between the referent group both normal (≥10.5...
BACKGROUND & AIMS: Patients with irritable bowel syndrome constipation (IBS-C) and patients functional (FC) have similar symptoms, these disorders overlap in their diagnostic features. Little is known about physiology or the involvement of serotonin signaling. We investigated relationships between platelet-depleted plasma concentrations serotonin, gastrointestinal motor-sensory function FC IBS-C compared healthy volunteers (controls). METHODS: We measured fasting fed individuals (n =...
Secondary hypogonadism is common in aging men; its natural history and predisposing factors are unclear.The objectives were 1) to identify that predispose eugonadal men (T ≥ 10.5 nmol/L) develop biochemical secondary < nmol/L; LH ≤ 9.4 U/L) hypogonadal recover eugonadism; 2) characterize clinical features associated with these transitions.The study was designed as a prospective observational general population cohort survey.The setting research centers.The participants 3369...
Low testosterone (T) has been associated with incident metabolic syndrome (MetS), but it remains unclear if this association is independent of sex hormone binding globulin (SHBG). Estradiol (E2) may also be MetS, few studies have investigated this.To study the between baseline steroids and development MetS to investigate influence SHBG, body mass index (BMI) insulin resistance on risk.Three thousand three hundred sixty nine community-dwelling men aged 40-79 years were recruited for...
Abstract Context: Measuring serum cortisol to evaluate stress, adrenal disease, and monitor hydrocortisone replacement requires venepuncture. Conversely, salivary measurements are noninvasive. Objective: This study aimed investigate measurement of cortisone as alternatives cortisol. Design Setting: was a prospective cross-over in clinical research facility. Patients Methods: Over three periods (Period 1, 24-h physiological rhythm; Periods 2 3, after 20 mg oral iv hydrocortisone) 14 male...
Polycystic ovary syndrome (PCOS) is a heterogeneous disease with many different aspects, including hyperandrogenism and metabolic disturbances. Clinical phenotypes show patterns of steroid hormones that have been investigated to some extent.This study intended determine the role testosterone (TT) dihydrotestosterone (DHT) ratio (TT/DHT ratio) in PCOS patients further assess correlation this hormonal, anthropometric, parameters.Serum samples 275 premenopausal fulfilling Rotterdam criteria 35...