Multidrug-resistant Klebsiella pneumoniae has emerged as one of the deadliest opportunistic nosocomial pathogens that forms biofilm for establishment chronic K. infections. Herein, we made an attempt to identify genes involved in formation strain ATCC13883. To achieve this, constructed mini-Tn5 transposon insertion mutants and screened them production. We observed was enhanced mutant where wcaJ gene disrupted. WcaJ is initiating enzyme colanic acid synthesis loads first sugar (glucose-1-P)...

Silk protein sericins, in the recent years, find application cosmetics and pharmaceuticals as biomaterials. We investigate potential of sericin, extracted from both mulberry Bombyx mori different non-mulberry sources, namely, tropical tasar, Antheraea mylitta ; muga, assama eri, Samia ricini, growth supplement serum-free culture medium. Sericin supplemented media containing concentrations sericins species are examined for attachment, growth, proliferation, morphology fibrosarcoma cells. The...

Mulberry silk fibroin is a widely used biomaterial and recent work on non-mulberry also suggests it may have similar uses. We expect from the only domesticated eri silkworm, Samia ricini, to possess useful properties as biomaterial. Eri gland heterodimeric protein of approximately 450 kDa. Cytocompatibility evaluation with fibroblasts osteoblast-like cells shows good cell attachment, viability proliferation. The matrices, which high thermal stability swellability, are haemocompatible....

Self-assembly processes are ubiquitous in natural systems, and their study can provide insight into the harnessing of unique properties for engineering new materials from bottom up. Models diffusion-limited assembly behavior have shown that structures formed a characteristic fractal dimensionality is smaller than embedding space or lattice. Typically however, these only been studied via theoretical computational tools, with relatively few systems having reported to approach limiting...

During the peptidoglycan (PG) maturation of mycobacteria, glycan strands are interlinked by both 3-3 (between two meso-diaminopimelic acids [meso-DAPs]) and 4-3 cross-links d-Ala meso-DAP), though there is a predominance (60 to 80%) cross-links. The dd-carboxypeptidases (dd-CPases) act on pentapeptides generate tetrapeptides that used ld-transpeptidases as substrates form Therefore, dd-CPases play crucial role in mycobacterial PG cross-link formation. However, physiology mycobacteria...

AmpG permease is implicated both in beta-lactamase induction and peptidoglycan recycling enterobacterial isolates. Here, physiological studies using molecular genetics show that deletion of dramatically increases beta-lactam susceptibility even the presence AmpC, TEM-1 OXA beta-lactamases. Also, there an appreciable decrease biofilm-forming ability strains lacking this protein. Expression excess probably compromises integrity bacterial cells, leading to cell lysis. Based on these results, we...

Bacterial surface components have a major role in the development of biofilms. In present study, effect Escherichia coli O8-antigen on biofilms was investigated using two E. K-12 derived strains that differed only biosynthesis. presence both bacterial adhesion and biofilm formation slightly decreased under static conditions whereas substantial increase observed agitated conditions. It noted that, irrespective status, hydrophobic interactions played an important However, conditions, extent...

Abstract With the growing threat of drug-resistant Acinetobacter baumannii , there is an urgent need to comprehensively understand physiology this nosocomial pathogen. As penicillin-binding proteins are attractive targets for antibacterial therapy, herein we have tried explore physiological roles two putative DD-carboxypeptidases, viz., dacC and dacD in A. . Surprisingly, deletion resulted a reduced growth rate, loss rod-shaped morphology, reduction biofilm-forming ability, enhanced...

Abstract With the growing threat of drug-resistant Acinetobacter baumannii, there is an urgent need to comprehensively understand physiology this nosocomial pathogen. As penicillin-binding proteins are attractive targets for antibacterial therapy, we have tried explore physiological roles two putative DD-carboxypeptidases, viz., DacC and DacD, in A. baumannii. Surprisingly, deletion dacC resulted a reduced growth rate, loss rod-shaped morphology, reduction biofilm-forming ability, enhanced...

Primary ovarian pregnancy occurs very rarely and usually in young women using intra uterine device. We present two case reports where primigravida females presented with pain abdomen were diagnosed as ectopic on ultrasonography confirmed histopathology examination Ovarian after partial oophorectomy.

Herpes esophagitis is a very rare disease caused by simplex virus (HSV). The occurs primarily in immunocompromised patients, including post-chemotherapy, immunosuppression with organ transplants, and AIDS patients but can also occur immunocompetent individuals. We report case of 57yrs old male presented complaint epigastric pain.