A5101874185- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Antifungal resistance and susceptibility
- Cardiac Ischemia and Reperfusion
- Bacterial Identification and Susceptibility Testing
- Osteoarthritis Treatment and Mechanisms
- Cardiovascular Function and Risk Factors
- Ophthalmology and Eye Disorders
- Bacillus and Francisella bacterial research
- Nitric Oxide and Endothelin Effects
- Click Chemistry and Applications
- Axon Guidance and Neuronal Signaling
- Bioactive natural compounds
- Proteoglycans and glycosaminoglycans research
- Antimicrobial Peptides and Activities
- Bacterial Genetics and Biotechnology
- Neuroscience and Neuropharmacology Research
- Ocular Infections and Treatments
- Synthesis and biological activity
- Photodynamic Therapy Research Studies
- Synthesis and Characterization of Heterocyclic Compounds
- Biochemical and Structural Characterization
Shanghai Medical College of Fudan University
2011-2022
The University of Tokyo
2020
Tokyo Medical and Dental University
2020
Fudan University
2020
Ningxia Medical University
2010-2012
Staphylococcus epidermidis (SE) has emerged as one of the most important causes nosocomial infections. The SaeRS two-component signal transduction system (TCS) influences virulence and biofilm formation in aureus. deletion saeR S. results impaired anaerobic growth decreased nitrate utilization. However, regulatory function on autolysis remains unclear.The saeRS genes SE1457 were deleted by homologous recombination. mutant, SE1457ΔsaeRS, exhibited increased that was disturbed more severely (a...
ABSTRACT SrrAB expression in Staphylococcus epidermidis strain 1457 (SE1457) was upregulated during a shift from oxic to microaerobic conditions. An srrA deletion (Δ ) mutant constructed for studying the regulatory function of SrrAB. The resulted retarded growth and abolished biofilm formation both vitro vivo under Associated with reduced formation, Δ produced much less polysaccharide intercellular adhesion (PIA) showed decreased initial adherence capacity. Microarray analysis that mutation...
Staphylococcus aureus (S. aureus) is hard to be eradicated, not only due the emergence of antibiotic resistant strains but also because its ability form biofilm. Antibiotics are major approach treating biofilm infections, their effects unsatisfactory. One potential alternative treatments for controlling infections photodynamic therapy (PDT), which requires administration photosensitizer, followed by light activation. 5-aminolevulinic acid (ALA), a natural photosensitizer prodrug, presents...
Methicillin-resistant Staphylococcus aureus (MRSA), also known as oxacillin-resistant S. aureus, is a leading cause of community and hospital associated infections globally. In this work, we found that deletion the arlRS two-component system genes in USA300 USA500 strains resulted increased susceptibilities to oxacillin (8-16-fold decrease minimal inhibitory concentrations). USA300ΔarlRS, transcriptional levels mecA or blaZ showed no obvious change, while mRNA spx 4-fold at 4 h 6.3-fold 10...
Abstract Objectives This study was designed to explore the effect and mechanism of matrine, an active component Chinese traditional medicine, on isoproterenol-induced acute cardiotoxicity in rats. Methods Acute myocardial injury induced rats by daily subcutaneous injection isoproterenol (85 mg/kg) for two days. Haemodynamic biochemical parameters were measured histopathological examination performed. Key findings Chronic oral administration matrine (50, 100 or 200 mg/kg per day 10 days)...
Abstract Objectives This study was designed to investigate the cardioprotective effects of matrine on regulation endothelial nitric oxide synthase (eNOS) and asymmetric dimethylarginine (ADMA) in isoproterenol-induced acute myocardial ischaemic rats. Methods Male Sprague–Dawley rats were pretreated with (200, 100 50 mg/kg) orally for 10 days. Acute injury induced by subcutaneous injection isoproterenol. Serum haemodynamic parameters, histopathological variables expression protein levels...
Staphylococcus epidermidis is a common bacterial colonizer of human skin and mucous membranes, yet it has emerged as an important nosocomial pathogen largely due to its ability form biofilms. Tobacco smoke been demonstrated contributor various infection diseases by improving the biofilm formation multiple species; however, association between tobacco S. still unclear. In this study, we tested effect nicotine, one most active components tobacco, on formation, studied underlying mechanisms....
Hyaluronic acid (HA) is a major molecular component of the articular cartilage temporomandibular joint (TMJ) influencing lubrication. Functional lateral shift mandible (FLSM) can lead to malocclusion. This study investigated effects FLSM on HA metabolism and lubrication TMJ in growing rats.Thirty 5-week-old male Wistar rats were divided into shift, recovery, control groups. Rats recovery groups fitted with guiding plates produce 2-mm which removed from group 14 days later. Animals sacrificed...
Previous reports have demonstrated two thiazolidione derivatives (H2-60 and H2-81) can robustly inhibit the planktonic growth biofilm formation of S. epidermidis aureus by targeting histidine kinase YycG. Whereas antibacterial anti-biofilm activity these against Enterococcus faecium remains elusive. Here, pET28a-YycG recombinant plasmid were in vitro expressed E. coli competent cell BL21 (DE3) induced to express YycG' protein (conding HisKA HATPase_c domain) 0.5 mM IPTG was purified Ni - NTA...
Abstract Background ClpP is important for bacterial growth and plays an indispensable role in cellular protein quality control systems by refolding or degrading damaged proteins, but the physiological significance of Enterococcus faecalis remains obscure. A clpP deletion mutant (△ ) was constructed using E. OG1RF strain to clarify effect on faecalis. The global abundance proteins determined a mass spectrometer with tandem tag labeling. Results Δ showed impaired at 20°C 45°C 5% NaCl 2 mM H O...