A5060148176- Hippo pathway signaling and YAP/TAZ
- RNA Research and Splicing
- Cancer-related gene regulation
- Blood Coagulation and Thrombosis Mechanisms
- Neonatal Respiratory Health Research
- Neuroendocrine regulation and behavior
- Reproductive System and Pregnancy
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Neuroscience of respiration and sleep
- Estrogen and related hormone effects
- Neonatal and fetal brain pathology
- Pneumocystis jirovecii pneumonia detection and treatment
- COVID-19 Impact on Reproduction
- Anesthesia and Neurotoxicity Research
- Bone health and osteoporosis research
- Autoimmune Bullous Skin Diseases
- Respiratory viral infections research
- Influenza Virus Research Studies
- Vitamin K Research Studies
- Venomous Animal Envenomation and Studies
- Pharmacogenetics and Drug Metabolism
- Maternal and Perinatal Health Interventions
- HIV/AIDS oral health manifestations
- Asthma and respiratory diseases
- Proteoglycans and glycosaminoglycans research
Washington University in St. Louis
2013-2024
Pulmonary and Critical Care Associates
2014
Lund University
1998-2002
Malmö University
2000
All India Institute of Medical Sciences
1995
Because NK cells secrete cytotoxic granules and cytokines that can destroy surrounding help shape the subsequent immune response, they must be kept under tight control. Several mechanisms, at different levels, are in place to control cell function. In this study, we describe a novel mechanism regulating function which acquire ligands for activating receptors from target by trogocytosis, rendering hyporesponsive. model, murine m157, CMV-encoded ligand Ly49H-activating receptor, both vitro...
Vitamin D deficiency at birth has been reported as a risk factor for respiratory syncytial virus (RSV) lower tract infection during the first year of life. Limited data are available on whether an infant's vitamin status is associated with severity acute RSV bronchiolitis. Infants < 1 age and hospitalized their episode bronchiolitis were enrolled into Bronchiolitis in Early Life II cohort. We investigated relationships between enrollment following indicators severity: duration...
In a head and neck squamous cell carcinoma (HNSCC) "window of opportunity" clinical trial, we reported that trametinib reduced MEK-Erk1/2 activation resulted in tumor responses subset patients. Here, investigated resistance to molecular correlates HNSCC lines patient samples.HNSCC were treated with generate resistant lines. Candidate bypass pathways assessed using immunoblotting, CRISPR knockout, survival assays. Effectiveness combined verteporfin targeting was evaluated. Patient-derived...
Despite six decades of the use exogenous oxytocin for management labor, little is known about its effects on developing brain. Motivated by controversial reports suggesting a link between during labor and autism spectrum disorders (ASDs), we employed our recently validated rat model induction with to address this important concern. Using combination molecular biological, behavioral, neuroimaging assays, show that induced birth leads sex-specific disruption oxytocinergic signaling in brain,...
Altered patterns of genetic expression induced by isoflurane preconditioning in mouse brain have not yet been investigated. The aim our pilot study is to examine the temporal sequence changes transcriptome cortex produced preconditioning.
A new method to determine the concentration of free protein S in plasma is described. It an enzyme-linked ligandsorbent assay (ELSA) which utilises binding capacity natural ligand C4b-binding (C4BP) capture from samples. The use C4BP as possible due high affinity (Kd = 0.1 nM) interaction between and a slow rate complex dissociation. monoclonal antibody (HPS 54) was conjugated with horseradish peroxidase used target antibody. This recognises Ca2+ dependent epitope first EGF-like domain does...
The impact of transient ischemic-hypoxemic insults on the developing fetal brain is poorly understood despite evidence suggesting an association with neurodevelopmental disorders such as schizophrenia and autism. To address this, we designed aberrant uterine hypercontractility paradigm oxytocin to better assess consequences acute, but transient, placental ischemia-hypoxemia in term pregnant rats. Using MRI, confirmed that oxytocin-induced substantially compromised uteroplacental perfusion....
Background Delayed cerebral ischemia remains a common and profound risk factor for poor outcome after subarachnoid hemorrhage (SAH). The aim of our current study is to define the role endothelial nitric oxide synthase (eNOS) in isoflurane conditioning‐induced neurovascular protection SAH. Methods Results Ten‐ 14‐week‐old male wild‐type mice (C57BL/6) as controls eNOS knockout (strain # 002684) were obtained study. Animals underwent either sham surgery, SAH or with conditioning. Anesthetic...
We recently reported that isoflurane conditioning provided multifaceted protection against subarachnoid hemorrhage (SAH)-induced delayed cerebral ischemia (DCI), and this was through the upregulation of endothelial nitric oxide synthase (eNOS). SIRT1, an NAD-dependent deacetylase, shown to be one critical regulators eNOS. The aim our current study is examine role SIRT1 in conditioning-induced neurovascular SAH-induced DCI. Mice were divided into four groups: sham, SAH, or SAH with (with...
The vitamin K-dependent anticoagulant protein S binds with high affinity to C4b-binding (C4BP), a regulator of complement. Despite the physiological importance complex, we have only patchy view C4BP-binding site in S. Based on phage display experiments, residues 447–460 were suggested form part binding site. Several experimental approaches now used further elucidate structural requirements for C4BP. Peptides comprising 447–460, 451–460, or 453–460 found inhibit S-C4BP interaction, whereas...
Low concentrations of serum vitamin K accompany high undercarboxylated osteocalcin (ucOC) and osteoporotic fractures. Although K2 (MK-4) is approved as a therapeutic agent for the treatment osteoporosis in some countries, dose-response unknown. The objective this study was to assess improvement carboxylation (OC) response escalating doses MK-4 supplementation. A nine-week, open-labeled, prospective cohort conducted 29 postmenopausal women who suffered hip or vertebral compression...
Despite the widespread use of oxytocin for induction labor, mechanistic insights into fetal/neonatal wellbeing are lacking because absence an animal model that recapitulates modern obstetric practice. Here, we create and validate a hi-fidelity pregnant rat mirrors labor with in laboring women. The consists implantable preprogrammed microprocessor-controlled infusion pump delivers gradually escalating dose intravenous to induce birth at term gestation. We validated molecular biological...
Summary Protein S is an important anticoagulant protein acting as cofactor to activated C (APC) in the degradation of membrane-bound factors Va and VIIIa. Binding membrane depends on Gla-domain, whereas sites for APC-interaction are located thrombin-sensitive region (TSR) first EGF domain. The aims present investigation were localize which involved APC-cofactor function elucidate possible orientations TSR relation membrane. For these purposes, we determined epitope a calcium-dependent...
Severe infection with respiratory syncytial virus (RSV) during infancy is strongly associated the development of asthma. To identify genetic variation that contributes to asthma following severe RSV bronchiolitis infancy, we sequenced coding exons 131 candidate genes in 182 European and African American children using anonymous pools for variant discovery, then directly genotyped a set 190 nonsynonymous variants. Association testing was performed physician-diagnosed before 7th birthday...
Use of maternal oxygen for intrauterine resuscitation is contentious because the lack evidence its efficacy and possibility fetal harm through oxidative stress. Because developing brain rich in lipids low antioxidants, it remains vulnerable to Here, we tested this hypothesis a term pregnant rat model with oxytocin-induced distress followed by treatment either room air or 100% 6 h. Fetal brains from both sexes were subjected assays biomarkers stress (4-hydroxynonenal, protein carbonyl,...