A5083959912- Genetics and Neurodevelopmental Disorders
- Autism Spectrum Disorder Research
- Retinal Development and Disorders
- Vestibular and auditory disorders
- Pharmacogenetics and Drug Metabolism
- Hearing, Cochlea, Tinnitus, Genetics
- Pain Mechanisms and Treatments
- Ion Channels and Receptors
- Distributed and Parallel Computing Systems
- Salmonella and Campylobacter epidemiology
- PARP inhibition in cancer therapy
- Genomics and Chromatin Dynamics
- Ion channel regulation and function
- Pain Management and Placebo Effect
- Electronic Health Records Systems
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Dental Education, Practice, Research
- Congenital heart defects research
- Flow Measurement and Analysis
- Memory and Neural Mechanisms
- Advanced Sensor Technologies Research
- Genomics and Rare Diseases
- Service-Oriented Architecture and Web Services
- Alzheimer's disease research and treatments
- Neuropeptides and Animal Physiology
University College London
2018-2024
King's College London
2024
Northeastern Illinois University
2022-2024
South London and Maudsley NHS Foundation Trust
2024
The University of Texas Rio Grande Valley
2024
Johns Hopkins University
2011-2021
Pakistan Institute of Nuclear Science and Technology
2021
Whitechapel Gallery
2018
University of London
2018
Johns Hopkins Medicine
2011-2015
The Shank3 gene encodes a scaffolding protein that anchors multiple elements of the postsynaptic density at synapse. Previous attempts to delete have not resulted in complete loss predominant naturally occurring isoforms. We now characterized homozygous mutation mice deletes exon 21, including Homer binding domain. In state, deletion 21 results major bands detected by C-terminal and N-terminal antibodies, allowing us more definitively examine role synaptic function behavior. This leads an...
Sensory integration difficulties have been reported in autism, but their underlying brain-circuit mechanisms are underexplored. Using five autism-related mouse models, Shank3+/ΔC, Mecp2R308/Y, Cntnap2−/−, L7-Tsc1 (L7/Pcp2Cre::Tsc1flox/+), and patDp(15q11-13)/+, we report specific perturbations delay eyeblink conditioning, a form of associative sensory learning requiring cerebellar plasticity. By distinguishing the probability characteristics learned responses, found that was reduced...
Background: Sensory neurons play an essential role in almost all pain conditions, and have recently been classified into distinct subsets on the basis of their transcriptomes. Here we analysed alterations dorsal root ganglia (DRG) gene expression using microarrays mouse models related to human chronic pain. Methods: Six different were studied male C57BL/6J mice: (1) bone cancer cell injection intramedullary space femur; (2) neuropathic partial sciatic nerve ligation; (3) osteoarthritis...
An unsuspected functional role of primary afferent neuron-generated sodium channel Na V 1.7 occurs in dorsal horn neurons.
The relationship between transcription and protein expression is complex. We identified polysome-associated RNA transcripts in the somata central terminals of mouse sensory neurons control, painful (plus nerve growth factor), pain-free conditions (Nav1.7-null mice). majority (98%) translated are shared male female mice both terminals. Some highly enriched or Changes translatome include novel known regulators pain pathways. Antisense knockdown selected somatic terminal that correlate with...
Mutations of SHANK3 cause Phelan-McDermid syndrome (PMS), and these individuals can exhibit sensitivity to stress, resulting in behavioral deterioration. Here, we examine the interaction stress with genotype using a mouse model face validity PMS. In Shank3ΔC/+ mice, swim produces an altered transcriptomic response pyramidal neurons that impacts genes pathways involved synaptic function, signaling, protein turnover. Homer1a, which is part Shank3-mGluR-N-methyl-D-aspartate (NMDA) receptor...
Pancreatic cancer (PanCa) is one of the deadliest cancers, with limited therapeutic response. Various molecular oncogenic events, including dysregulation ribosome biogenesis, are linked to induction, progression, and metastasis PanCa. Thus, discovery new therapies suppressing these events biogenesis could be a novel approach for prevention treatment The current study was designed investigate anti-cancer effect honey against Our results indicated that markedly inhibited growth invasive...
Aims Local protein synthesis at the synapse is a key determinant of learning and memory predicted to be severely disrupted in Alzheimer's disease (AD). Omics approaches have played role deciphering molecular mechanisms underlying AD pathology. However, isolating transcriptome may biased due inherent variations transcript levels, or by transcription-on-demand models employed several genes, whereas mass-spec based proteomics fail capture low abundance peptides. The translatome bypasses these...
A lack of knowledge the detailed behavior actual transducers used in a particular application can provide an indeterminate limitation on sensitivity or accuracy measurements being made. In principle, distribution surface vibration transducer permits irradiating field at any point region interest to be determined. Previous reports determination have suffered from verification. The present work made four (two with ceramic elements and two PVdF approximate diameter 10 mm frequencies between...