A5042088440- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Microbial Metabolism and Applications
- Fungal Biology and Applications
- Enzyme-mediated dye degradation
- DNA Repair Mechanisms
Medizinische Hochschule Hannover
2016-2023
Leibniz University Hannover
2017
Identification of cancer-specific target molecules and biomarkers may be useful in the development novel treatment immunotherapeutic strategies. We have recently demonstrated that expression long noncoding (lnc) RNAs can cancer-type specific due to abnormal chromatin remodeling alternative splicing. Furthermore, we identified determined functional small protein C20orf204-189AA encoded by intergenic RNA Linc00176 is expressed predominantly hepatocellular carcinoma (HCC), enhances...
Abstract In most human cancers, a large number of proteins with driver mutations are involved in tumor development, implying that multiple fine tuners cancer formation and/or maintenance. A useful strategy for therapy may therefore be to target type-specific tuners. Furthermore, genome-wide association studies samples have identified long noncoding (lnc)RNA associated various types tumor. this context we previously found C20orf204 (a splice variant Linc00176) RNA contains 189 amino acid (AA)...
THOC5, a member of the THO complex, is essential for 3'processing some inducible genes, export subset mRNAs and stem cell survival. Here we show that THOC5 depletion results in altered 3'cleavage >50% changes RNA polymerase II binding across genes. recruited close to high-density sites, suggesting involved transcriptional elongation. Indeed, measurement elongation rates vivo demonstrated decreased THOC5-depleted cells. Furthermore, preferentially its target genes slow cells compared with...
Abstract The Myc gene has been implicated in the pathogenesis of most types human cancerous tumors. Myc/Max activates large numbers pro-tumor genes; however it also induces anti-proliferation genes. When genes are activated by Myc, cancer cells can only survive if they downregulated. Hepatocellular carcinoma (HCC) specific intronic long noncoding antisense (lnc-AS) RNA, EVA1A-AS gene, is located within second intron (I2) EVA1A (EVA-1 homolog A) that encodes an factor. Indeed, EVA1A, but not...
The vast majority of patients at risk hereditary breast and/or ovarian cancer (HBOC) syndrome remain without a molecular diagnosis after routine genetic testing. One type genomic alteration that is commonly missed by diagnostic pipelines mobile element insertions (MEIs). Here, we reanalyzed multigene panel data from suspected HBOC using the MEI detection tool Mobster. A novel Alu insertion in ATM intron 54 (ATM:c.8010+30_8010+31insAluYa5) was identified as potential contributing factor seven...
Alternative cleavage and polyadenylation (APA) is a widespread mechanism to generate mRNA isoforms with alternative 3' untranslated regions. Here, we detail protocol for detecting APA genome wide using direct RNA sequencing technology including computational analysis. We describe steps sample library preparation, nanopore sequencing, data Experiments analysis can be performed over period of 6-8 days require molecular biology bioinformatics skills. For complete details on the use execution...
THOC5, a member of the THO complex, is essential for 3´processing some inducible genes, export subset mRNAs and stem cell survival. Here we show that THOC5 depletion results in altered 3´cleavage >50% changes RNA Polymerase II binding across genes. recruited close to high density sites suggesting involved transcriptional elongation. Indeed, measurement elongation rates vivo demonstrated decreased depleted cells. Furthermore, preferentially its target genes slow cells compared fast...