A5044146322- RNA Research and Splicing
- RNA modifications and cancer
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Immune cells in cancer
- Genomics and Chromatin Dynamics
- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- DNA Repair Mechanisms
- Hepatocellular Carcinoma Treatment and Prognosis
- Organ Transplantation Techniques and Outcomes
- RNA and protein synthesis mechanisms
German Cancer Research Center
2021-2024
Heidelberg University
2021-2024
Cancer Research Center
2022
Medizinische Hochschule Hannover
2021
Identification of cancer-specific target molecules and biomarkers may be useful in the development novel treatment immunotherapeutic strategies. We have recently demonstrated that expression long noncoding (lnc) RNAs can cancer-type specific due to abnormal chromatin remodeling alternative splicing. Furthermore, we identified determined functional small protein C20orf204-189AA encoded by intergenic RNA Linc00176 is expressed predominantly hepatocellular carcinoma (HCC), enhances...
Chronic liver diseases are worldwide on the rise. Due to rapidly increasing incidence, in particular Western countries, metabolic dysfunction-associated steatotic disease (MASLD) is gaining importance as can develop into hepatocellular carcinoma. Lipid accumulation hepatocytes has been identified characteristic structural change MASLD development, but molecular mechanisms responsible for progression remained unresolved. Here, we uncover primary from a preclinical model fed with diet (WD) an...
Abstract In most human cancers, a large number of proteins with driver mutations are involved in tumor development, implying that multiple fine tuners cancer formation and/or maintenance. A useful strategy for therapy may therefore be to target type-specific tuners. Furthermore, genome-wide association studies samples have identified long noncoding (lnc)RNA associated various types tumor. this context we previously found C20orf204 (a splice variant Linc00176) RNA contains 189 amino acid (AA)...
THOC5, a member of the THO complex, is essential for 3'processing some inducible genes, export subset mRNAs and stem cell survival. Here we show that THOC5 depletion results in altered 3'cleavage >50% changes RNA polymerase II binding across genes. recruited close to high-density sites, suggesting involved transcriptional elongation. Indeed, measurement elongation rates vivo demonstrated decreased THOC5-depleted cells. Furthermore, preferentially its target genes slow cells compared with...
Abstract Type I interferons (IFNs) play a central role not only in innate immunity against viral infection, but also the antitumour response, e.g. through direct impact on cell proliferation. Particularly for cancer arising context of chronic inflammation, constant exposure to IFNs may constitute strong selective pressure during tumour evolution. Expansion neoplastic subclones resistant antiproliferative effects contribute immunoediting tumours, leading more aggressive disease. Experimental...
Alternative cleavage and polyadenylation (APA) is a widespread mechanism to generate mRNA isoforms with alternative 3' untranslated regions. Here, we detail protocol for detecting APA genome wide using direct RNA sequencing technology including computational analysis. We describe steps sample library preparation, nanopore sequencing, data Experiments analysis can be performed over period of 6-8 days require molecular biology bioinformatics skills. For complete details on the use execution...
THOC5, a member of the THO complex, is essential for 3´processing some inducible genes, export subset mRNAs and stem cell survival. Here we show that THOC5 depletion results in altered 3´cleavage >50% changes RNA Polymerase II binding across genes. recruited close to high density sites suggesting involved transcriptional elongation. Indeed, measurement elongation rates vivo demonstrated decreased depleted cells. Furthermore, preferentially its target genes slow cells compared fast...
Abstract Chronic liver diseases are worldwide on the rise. Due to rapidly increasing incidence, in particular Western countries, Non-alcoholic fatty disease (NAFLD) is gaining importance as can develop into hepatocellular carcinoma. Lipid accumulation hepatocytes has been identified characteristic structural change NAFLD development, but molecular mechanisms responsible for progression remained unresolved. Here, we uncover primary from a preclinical model fed with diet (WD) strong...
Abstract Type I interferons (IFNs) play a central role not only in innate immunity against viral infection, but also the antitumour response. Apart from indirect immune-modulatory and anti-angiogenic effects, they have direct impact on cell proliferation. Particularly for cancer arising context of chronic inflammation, constant exposure to IFNs may constitute strong selective pressure during tumour evolution. Expansion neoplastic subclones or -populations that developed resistance...