A5039153883- Microtubule and mitosis dynamics
- Genomics and Chromatin Dynamics
- Advanced Fluorescence Microscopy Techniques
- Telomeres, Telomerase, and Senescence
- DNA Repair Mechanisms
- Cell Image Analysis Techniques
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Cancer-related Molecular Pathways
- Genomic variations and chromosomal abnormalities
- RNA modifications and cancer
- Gene Regulatory Network Analysis
- Digital Holography and Microscopy
- Metabolism and Genetic Disorders
- Cellular Mechanics and Interactions
- Plant nutrient uptake and metabolism
- Mitochondrial Function and Pathology
- PARP inhibition in cancer therapy
- Genetics, Aging, and Longevity in Model Organisms
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Epigenetics and DNA Methylation
- Metabolomics and Mass Spectrometry Studies
ETH Zurich
2011-2024
Institute for Biomedical Engineering
2023
Howard Hughes Medical Institute
2018-2023
Massachusetts Institute of Technology
2018-2023
Centre for Genomic Regulation
2016
Board of the Swiss Federal Institutes of Technology
2011
Universitat Pompeu Fabra
2011
Cell size varies greatly between cell types, yet within a specific type and growth condition, is narrowly distributed. Why maintenance of cell-type important remains poorly understood. Here we show that growing budding yeast primary mammalian cells beyond certain impairs gene induction, cell-cycle progression, signaling. These defects are due to the inability large scale nucleic acid protein biosynthesis in accordance with volume increase, which effectively leads cytoplasm dilution. We...
Cellular senescence refers to an irreversible state of cell-cycle arrest and plays important roles in aging cancer biology. Because is associated with increased cell size, we used reversible arrests combined growth rate modulation study how excessive affects proliferation. We find that enlarged cells upregulate p21, which limits progression. Cells re-enter the cycle encounter replication stress well tolerated physiologically sized but causes severe DNA damage cells, ultimately resulting...
Partitioning of chromatids during mitosis requires that chromosome compaction and spindle length scale appropriately with each other. However, it is not clear whether condensation elongation are linked. Here, we find yeast cells could cope a 45% increase in the their longest arm by increasing its condensation. The midzone, aurora/Ipl1 activity, Ser10 histone H3 mediated this response. Thus, anaphase may function as ruler to adapt chromatids, promoting segregation regardless or length.
Budding yeast cells produce a finite number of daughter before they die. Why old stop dividing and die is unclear. We found that age-induced accumulation the G1/S-phase inhibitor Whi5 defects in G1/S cyclin transcription cause cell cycle delays genomic instability result death. further identified extrachromosomal rDNA (ribosomal DNA) circles (ERCs) to expression defect cells. Spontaneous segregation ERCs into rejuvenates mothers, but daughters inherit these aging factors rapidly. Our results...
Prolonged cell cycle arrests occur naturally in differentiated cells and response to various stresses such as nutrient deprivation or treatment with chemotherapeutic agents. Whether how survive prolonged is not clear. Here, we used
The precise, quantitative evaluation of intracellular organelles in three-dimensional (3D) imaging data poses a significant challenge due to the inherent constraints traditional microscopy techniques, requirements use exogenous labeling agents, and existing computational methods. To counter these challenges, we present hybrid machine-learning framework exploiting correlative 3D phase with fluorescence labeled cells. algorithm, which synergistically integrates random-forest classifier deep...
Abstract Cellular senescence plays an important role in development, ageing, and cancer biology. Senescence is associated with increased cell size, but how this contributes to permanent cycle exit poorly understood. Using reversible G1 arrests combined growth rate modulation, we examined the effects of excess size on progression human cells. We show that enlarged cells paradoxically have high levels G1/S regulators relative were maintained at physiological also induce p21, which restrains...
Abstract The precise, quantitative evaluation of intracellular organelles in three-dimensional (3D) imaging data poses a significant challenge due to the inherent constraints traditional microscopy techniques, requirements use exogenous labeling agents, and existing computational methods. To counter these challenges, we present hybrid machine-learning framework exploiting correlative 3D phase with fluorescence labeled cells. algorithm, which synergistically integrates random-forest...
Abstract Temperature sensitive cell division cycle ( cdc-ts ) cells are unable to progress through the at restrictive temperature due mutations in genes essential progress. Cells harboring increase volume upon arrest but eventually stop growing. We found that this attenuation growth was selective downregulation of ribosome concentration. saw similar arrested alpha factor addition, rapamycin and entrance into stationary phase. In all arrests studied, activated Environmental Stress Response...
Cellular senescence plays an important role in development, ageing, and cancer biology. Senescence is associated with increased cell size, but how this contributes to permanent cycle exit poorly understood. Using reversible G1 arrests combined growth rate modulation, we examined the effects of excess size on progression human cells. We show that enlarged cells paradoxically have high levels G1/S regulators relative were maintained at physiological also induce p21, which restrains entry...