A5110245364- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Receptor Mechanisms and Signaling
- Neural dynamics and brain function
- Neuroinflammation and Neurodegeneration Mechanisms
- Memory and Neural Mechanisms
- Nicotinic Acetylcholine Receptors Study
- Neurotransmitter Receptor Influence on Behavior
- Ion channel regulation and function
- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- Single-cell and spatial transcriptomics
- RNA regulation and disease
- Wireless Sensor Networks and IoT
- Virus-based gene therapy research
- Epilepsy research and treatment
- Bone and Joint Diseases
- Immune cells in cancer
- Metal-Organic Frameworks: Synthesis and Applications
- Bone health and treatments
- Photochromic and Fluorescence Chemistry
- Technology Assessment and Management
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2001-2025
National Institutes of Health
2006-2025
Government of the United States of America
2024
Emory University
2021
Soochow University
2020
Fourth People's Hospital of Changzhou
2020
Eunice Kennedy Shriver Center
2015-2019
Synaptic Research (United States)
2019
Institute of Developmental Physiology
2015
China Agricultural University
2011-2014
Neurogenesis is known to persist in the adult mammalian central nervous system (CNS). The identity of cells that generate new neurons postnatal CNS has become a crucial but elusive issue. Using transgenic mouse, we show NG2 proteoglycan–positive progenitor express 2′,3′-cyclic nucleotide 3′-phosphodiesterase gene display multipotent phenotype vitro and electrically excitable neurons, as well astrocytes oligodendrocytes. fast kinetics high rate fate these NG2+ progenitors reflect an intrinsic...
ABSTRACT We tested the hypothesis that neurotransmitter glutamate would influence glial proliferation and differentiation in a cytoarchitecturally intact system. Postnatal day 6 cerebellar slices were maintained organotypic culture treated with receptor agonists or antagonists. After dissociation, cells stained antibodies for different oligodendrocyte developmentally regulated antigens. Treatment of kainate α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid significantly decreased...
SUMMARY In recent years, we and others have identified a number of enhancers that, when incorporated into rAAV vectors, can restrict the transgene expression to particular neuronal populations. Yet, viral tools access manipulate fine subtypes are still limited. Here, performed systematic analysis single cell genomic data identify enhancer candidates for each cortical interneuron subtypes. We established set enhancer-AAV that highly specific distinct populations striatal cholinergic neurons....
Abstract We generated a transgenic mouse expressing the enhanced green fluorescent protein (EGFP) under control of 2′‐3′‐cyclic nucleotide 3′‐phosphodiesterase (CNP) promoter. EGFP + cells were visualized in live tissue throughout embryonic and postnatal development. Immunohistochemical analysis brain sciatic nerve demonstrated that expression was restricted to oligodendrocyte Schwann cell lineages. also strongly expressed “adult” progenitors (OPs) gray matter oligodendrocytes....
Neural cell development is regulated by membrane ion channel activity. We have previously demonstrated that depolarization with veratridine or blockage of K+ channels tetraethylammonium (TEA) inhibit oligodendrocyte progenitor (OP) proliferation and differentiation (); however the molecular events involved are largely unknown. Here we show forskolin (FSK) its derivative dideoxyforskolin (DFSK) block in OPs proliferation. The antiproliferative effects TEA, FSK, DFSK, were attributable to OP...
Abstract N-methyl-D-aspartate receptors (NMDARs) are ligand-gated ionotropic glutamate that mediate a calcium-permeable component to fast excitatory neurotransmission. NMDARs heterotetrameric assemblies of two obligate GluN1 subunits ( GRIN1 ) and GluN2 GRIN2A - GRIN2D ). Sequencing data shows 43% (297/679) all currently known NMDAR disease-associated genetic variants within the gene, which encodes GluN2A subunit. Here, we show unlike missense variants, individuals affected with null...
Abstract We examined the pathways that link neurotransmitter receptor activation and cell cycle arrest in oligodendrocyte progenitors. had previously demonstrated glutamate inhibits progenitor proliferation lineage progression. Here, using purified progenitors cerebellar slice cultures, we show norepinephrine β-adrenergic agonist isoproterenol also inhibited proliferation, but contrast to glutamate, stimulated progression, as determined by O4 O1 antibody staining. This antiproliferative...
In violation of Dale’s principle several neuronal subtypes utilize more than one classical neurotransmitter. Molecular identification vesicular glutamate transporter three and cholecystokinin expressing cortical interneurons (CCK+VGluT3+INTs) has prompted speculation GABA/glutamate corelease from these cells for almost two decades despite a lack direct evidence. We unequivocally demonstrate CCK+VGluT3+INT-mediated cotransmission onto principal in adult mice using paired recording optogenetic...
Although Netos are considered auxiliary subunits critical for kainate receptor (KAR) function, direct evidence their regulation of native KARs is limited. Because Neto KAR GluK subunit/Neto isoform specific, such must be determined in cell-type-specific contexts. We demonstrate Neto1/2 expression somatostatin (SOM)-, cholecystokinin/cannabinoid 1 (CCK/CB1)-, and parvalbumin (PV)-containing interneurons. KAR-mediated excitation these interneurons contingent upon Neto1 because yields...
Abstract Using the previously cloned proteasome α-type subunit gene Pros28.1, we screened a Drosophila melanogaster genomic library using reduced stringency conditions to identify closely related genes. Two new genes, Pros28.1A (map position 92F) and Pros28.IB 60D7), showing high sequence similarity were identified characterized. encodes protein with 74% amino acid identity PROS28.1, while Pros28.1B product is 58% identical. The has two introns, located in exactly analogous positions as...
The medial habenula (mHb)/interpeduncular nucleus (IPN) circuitry is resident to divergent molecular, neurochemical and cellular components which, in concert, perform computations drive emotion, reward addiction behaviors. Although housing one of the most prominent mu opioid receptor (mOR) expression levels brain, remarkably little known as how they impact mHb/IPN circuit function at granular level. In this study, our systematic functional pharmacogenetic analyses demonstrate that mOR...
The medial habenula (mHb)/interpeduncular nucleus (IPN) circuitry is resident to divergent molecular, neurochemical and cellular components which, in concert, perform computations drive emotion, reward addiction behaviors. Although housing one of the most prominent mu opioid receptor (mOR) expression levels brain, remarkably little known as how they impact mHb/IPN circuit function at granular level. In this study, our systematic functional pharmacogenetic analyses demonstrate that mOR...
Proliferation of oligodendrocyte progenitor (OP) cells is a crucial process controlling myelination in the CNS. Previous studies demonstrated correlation between OP proliferation rate and cyclin E/cyclin-dependent kinase-2 (cdk2) activity. To establish causal link E/cdk2 activity proliferation, we selectively modulated cdk2 <i>in vitro</i> by transfection cultured cells. Dominant-negative (Dn)-cdk2 overexpression inhibited mitogen-induced cell whereas wild-type (wt)-cdk2 prevented cycle...
Opioid receptors within the CNS regulate pain sensation and mood are key targets for drugs of abuse. Within adult rodent hippocampus (HPC), μ-opioid receptor agonists suppress inhibitory parvalbumin-expressing interneurons (PV-INs), thus disinhibiting circuit. However, it is uncertain if this disinhibitory motif conserved in other cortical regions, species, or across development. We observed that PV-IN mediated inhibition robustly suppressed by opioids HPC but not neocortex mice nonhuman...
Medial ganglionic eminence (MGE)-derived parvalbumin (PV)+, somatostatin (SST)+and Neurogliaform (NGFC)-type cortical and hippocampal interneurons, have distinct molecular, anatomical, physiological properties. However, the molecular mechanisms regulating their maturation remain poorly understood. Here, via single-cell transcriptomics, we show that obligate NMDA-type glutamate receptor (NMDAR) subunit gene Grin1 mediates transcriptional regulation of expression in specific subtypes...